Application of the comet and micronucleus assays to butterfish (Pholis gunnellus) erythrocytes from the Firth of Forth, Scotland

Bombail, Vincent; Aw, D. W. J.; Gordon, Emma; Batty, Jennifer

doi:10.1016/s0045-6535(00)00300-3

Cited by 144 publications

(92 citation statements)

References 31 publications

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“…The lower sensitivity of MNT for various feral fi sh species has also been described elsewhere (53). In contrast, butterfi sh (Pholis gunnellus) from contaminated areas in Firth of Forth (Scotland) showed higher MN frequency but no increase in DNA strand breaks measured by the comet assay (54). Similar was reported for three-spined sticklebacks (G. aculeatus) (48).…”

Section: Discussionsupporting

confidence: 66%

Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

Šrut

Štambuk

Pavlica

et al. 2010

Archives of Industrial Hygiene and Toxicology

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Genotoxic effects are often the earliest signs of pollution-related environmental disturbance. In this study, we used the comet assay and micronucleus test to assess DNA damage in the erythrocytes of the European sea bass (Dicentrarchus labrax) exposed to environmental pollution in situ. Fish were collected from a fi sh farm in the Trogir Bay and their cages placed at an unpolluted reference site Šolta (Nečujam Bay) and a polluted site Vranjic (Kaštela Bay) for four weeks. A group of fi sh which remained at the fi sh farm Trogir Bay were used as the second control group. Fish exposed at the Vranjic site showed a signifi cantly higher erythrocyte DNA damage, measured by the comet assay, than either control group. Micronucleus induction showed a similar gradient of DNA damage, but did not reach statistical signifi cance. Our results show that cage exposure of a marine fi sh D. labrax can be useful in environmental biomonitoring and confi rm the comet assay as a suitable tool for detecting pollution-related genotoxicity.

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Section: Discussionsupporting

confidence: 66%

Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

Šrut

Štambuk

Pavlica

et al. 2010

Archives of Industrial Hygiene and Toxicology

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“…Two herbicides, 2,4-dichlorophenoxyacetic acid and butachlor induced dose and time dependant genotoxic and cytotoxic effects in peripheral erythrocytes of catfish (Clarias batrachus) (Ateeq et al, 2002). In eel treatment with 0.3, 0.9 and 2.7 µM of naphtalene caused induction of MN and other nuclear abnormalities (Teles et al, 2003).Elevated frequencies of erythrocytes with MN, lobed and blebbed nuclei were observed in butterfish (Pholis gunnellus) inhabiting contaminated areas of the Firth of Forth, Scotland (Bombail et al, 2001). Increased levels of environmental genotoxicity and cytotoxicity were observed in polluted areas off the southeastern Mediterranean coast of Turkey.…”

Section: Discussionmentioning

confidence: 99%

“…In the last decades, environmental genotoxicity in coastal areas of the Baltic and the North Seas was assessed in the number of studies (Al-Sabti and Hardig, 1990;Bresler et al, 1999;Baršienė et al, 2000, Thiriot-Quiévreux and Wolowicz, 2001Bombail et al, 2001;Frenzilli et al, 2004;Schiedek et al, 2006;Kopecka et al, 2006;Broeg and Lehtonen, 2006;Lehtonen et al, 2006;Stachnik and Wolowicz, 2007;Köhler and Ellesat, 2008). However, there is a lack of information on genotoxic and cytotoxic effects in the offshore areas of the Baltic and the North Seas.…”

Section: Introductionmentioning

confidence: 99%

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Rybakovas

Baršienė

Lang

2009

Marine Environmental Research

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Please cite this article as: Rybakovas, A., Baršienė, J., Lang, T., Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and North Seas, Marine Environmental Research (2009Research ( ), doi: 10.1016Research ( /j.marenvres. 2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractMicronuclei (MN), nuclear buds (NB) and fragmented-apoptotic cells (FA) were analyzed in mature peripheral blood and immature cephalic kidney erythrocytes of flounder (Platichthys flesus), dab (Limanda limanda) and cod (Gadus morhua) from 12 offshore sites in the Baltic Sea (479 specimens) and 11 sites in the North Sea (291 specimens), which were collected during three research vessel cruises in December 2002 and in September 2004. The highest levels of environmental genotoxicity (frequencies of MN up to 0.5 ‰, NB -up to 0.75 ‰) and cytotoxicity (FA -up to 0.53 ‰) were observed in flatfishes from areas close to oil and gas platforms in the North Sea and in zones related to the extensive shipping and potentially influenced by contamination from large European Rivers (Elbe, Vistula, Oder). In dab from the offshore zones of the North Sea, the levels of nuclear abnormalities were higher as compared to those in dab from the Baltic Sea. Responses in immature kidney erythrocytes were higher than in mature erythrocytes from peripheral blood. MN frequency lower than 0.05 ‰ (the Baltic Sea) and lower than 0.1 ‰ (the North Sea) could be suspected as a reference level in the peripheral blood erythrocytes of flatfish.Keywords: Genotoxicity; Cytotoxicity; Micronuclei; Nuclear abnormalities; Erythrocytes; Baltic Sea; North Sea. ACCEPTED MANUSCRIPT 2 IntroductionSurrounded by European countries, the Baltic and the North Seas are constantly exposed to anthropogenic pressure. With pollution from land-based discharges, coastal industries, municipal outflows, agricultural activities, oil and gas industry (drilling and transportation) and transport activities, thousands of chemicals are entering the marine environment. A large proportion of these compounds are potentially toxic, genotoxic and carcinogenic to aquatic organisms (Jha, 2004(Jha, , 2008. The interaction of genotoxic contaminants with DNA causes various genetic disturbances, which often are irreversible and can be transmitted to the next generations (Depledge and Fossi, 1994;Dixon et al., 1999;Jha, 2004). The analysis of environmental genotoxicity provides early warning signs of adverse long-term effects of contamination. A number of biomarkers have been applied for assessment of environmental genotoxicity and the micronuclei (MN) test,...

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“…Other investigators have also reported nuclear abnormalities but simply classified them as 'genotoxic damage' (Bombail et al, 2001;Pachecco and Santos, 1998;Ayylon and Garcia-Vazquez, 2000). Eiras (1990) identified nuclear abnormalities in fish with folic acid deficiency or which had been affected by viral necrosis, as well as in specimens exposed to chlorine and cadmium.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips

et al. 2006

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Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium compounds. The greatest comet assay damage occurred with water from a chromium-containing tannery effluent discharge site, supporting the hypothesis that chromium residues can be genotoxic. The mutagenicity of the water samples was assessed using the onion root-tip cell assay, the most frequent chromosomal abnormalities observed being: c-metaphases, stick chromosome, chromosome breaks and losses, bridged anaphases, multipolar anaphases, and micronucleated and binucleated cells. Onion root-tip cell mutagenicity was highest for water samples containing the highest levels of chromium.

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Application of the comet and micronucleus assays to butterfish (Pholis gunnellus) erythrocytes from the Firth of Forth, Scotland

Cited by 144 publications

References 31 publications

Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

Cage Exposure of European Sea Bass (Dicentrarchus Labrax) for in Situ Assessment of Pollution-Related Genotoxicity

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips

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