Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation

Kruszewski, Marcin; Wojewódzka, Maria; Iwaneńko, Teresa; Collins, Andrew; Szumiel, Irena

doi:10.1016/s1383-5718(98)00074-6

Cited by 44 publications

(23 citation statements)

References 13 publications

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“…Single strand DNA breaks were estimated according to the standard protocol described in detail by Wojewódzka et al (1998). 8-oxoG was estimated according to the protocol described by Kruszewski et al (1998).…”

Section: Comet Assaymentioning

confidence: 99%

Micronucleus test and comet assay on mice fed over five generations a diet containing genetically modified triticale

et al. 2008

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One of the concerns regarding the common use of genetically modifi ed (GM) feed in animal nutrition is that transgenic sequences may have a negative effect on the organism or (and) its cells. The present report assesses the genotoxic potential effect of a diet containing GM triticale on mice by using micronuclei test and comet assay. One group of mice (C57 Bl/6J strain) were fed continuously over fi ve generations a pelleted diet containing 20% of GM triticale (tolerant to phosphinothricine) grain, while the control group was fed pellets with 20% conventional triticale grain. Ten 91-days-old mice (fi ve females and fi ve males) were randomly selected from each group and each generation for micronuclei test in bone marrow and peripheral blood erythrocytes and the some number of mice was used for comet assay. The results obtained did not reveal any statistically signifi cant differences in the micronuclei frequency nor any other DNA damage between the control and experimental groups of mice in all fi ve generations. Thus, it seems evident that the diet containing GM triticale (with bar transgene) does not induce chromosome damage, nor has it any effect on the formation of DNA breaks or base lesions.

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Section: Comet Assaymentioning

confidence: 99%

Micronucleus test and comet assay on mice fed over five generations a diet containing genetically modified triticale

et al. 2008

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“…The alkaline comet assay was performed according to the standard protocol described in detail in Wojewódzka et al (1998). The FPG modified comet assay was described in Kruszewski et al (1998).…”

Section: Comet Assaymentioning

confidence: 99%

“…The FPG modified comet assay was described in Kruszewski et al (1998). In brief, microscope slides were precoated with 100 ml agarose (0.5% Agarose Type I-A (Sigma), in redistilled water) and dried.…”

Section: Comet Assaymentioning

confidence: 99%

DNA damage and its repair in lymphocytes and thyroid nodule cells during radioiodine therapy in patients with hyperthyroidism

Grzesiuk¹,

Nieminuszczy²,

Kruszewski³

et al. 2006

Journal of Molecular Endocrinology

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Radioiodine treatment of hyperthyroid patients with autonomous thyroid nodule leads to cellular DNA damage not only in thyrocytes but also in peripheral blood lymphocytes. The purpose of this study was to evaluate DNA breakage and base damage in thyrocytes and lymphocytes in patients treated with 131-I. In all the patients thyroid scintiscan was performed using 131-I. Damage to DNA was estimated by comet assay. Samples were taken before radioiodine treatment, and 12 and 54 days afterwards. Our results indicate high diversity in the level of DNA damage among the individual patients. However, in all cases, after 54 days the level of DNA damage in lymphocytes was similar or even lower than that in the controls. In contrast, in hot nodule the DNA damage persisted until the 54th day after 131-I application. Differences in the type of DNA damage between thyrocytes and lymphocytes were also observed. In lymphocytes there was more base damage, whereas in thyrocytes single strand breaks prevailed. This may indicate different mechanisms of DNA damage induction and/or DNA repair.

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“…There are few studies that assess the genetic effects of human in vivo exposure to radiation using lymphocytes from the peripheral blood. The available data include studies carried out on individuals occupationally exposed to high doses 11,3 ; patients 27 ; people who were accidentally exposed 20,18,6,23 and persons occupationally exposed to low doses of ionizing radiation 9,13,28 . In Brazil, a large group of dentists who are not specialists in radiology deal in their offices with low doses of ionizing radiation without utilizing dosimeters.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic biomonitoring of Brazilian dentists occupationally exposed to low doses of X-radiation

Miyaji

Cólus

2002

Pesqui. Odontol. Bras.

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Exposure to X-rays (ionizing radiation) may cause chromosomal aberrations (CA) in somatic or germinative cells in exposed individuals, and may lead to manifestations of diseases such as cancer. This study was carried out to assess the CA frequency in lymphocytes obtained by means of temporary culture of peripheral blood from dentists in Londrina, Paraná, Brazil, who have worked for more than ten years with X-rays. The results obtained from the experimental group were compared with a matched negative control group, which had never been exposed to X-rays. All individuals, dentists and controls, answered a personal questionnaire, from which a profile of each group was obtained. Slides, prepared after the cultures, were stained with Giemsa, and 100 to 200 metaphase cells were analyzed per individual. CA frequencies and types were registered and statistical tests were not necessary to evaluate the obtained data. The analysis of mitotic index (MI) did not indicate significant differences (p < 0.05) between the group of individuals exposed to X-rays and the control group. The analyzed confounding factors did not influence the results of MI and CA frequencies. UNITERMS: Chromosome aberrations; Occupational exposure; Mitotic index; Dentist's practice patterns; X-rays. RESUMO:A exposição à radiação ionizante pode causar aberrações cromossômicas (AC) em células somáticas ou germinativas dos indivíduos expostos, podendo levar a manifestações de doenças como os cânceres. Este trabalho teve como objetivo avaliar a freqüência de AC em linfócitos obtidos através de cultura temporária do sangue periférico de dentistas de Londrina (Paraná -Brasil), com mais de dez anos de trabalho com raios X. Os resultados obtidos do grupo experimental foram comparados com um grupo controle negativo pareado, que nunca se expôs ocupacionalmente aos raios X. Todos os indivíduos, dentistas e controles, responderam a um questionário pessoal, a partir do qual foi obtido um perfil de cada grupo. Após as culturas, as lâminas foram confeccionadas, coradas com solução de Giemsa e foram analisadas de 100 a 200 células metafásicas por indivíduo, anotando-se as freqüências e tipos de AC encontrados. Não foi necessária a realização de testes estatísticos para avaliação desses parâmetros. A análise dos índices mitóticos (IM) não indicou diferenças significantes (p < 0,05) entre os indivíduos dos grupos expostos e controle. Os fatores de variação analisados não influenciaram os resultados obtidos para o IM e freqüência de AC.

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Application of the comet assay for monitoring DNA damage in workers exposed to chronic low-dose irradiation

Cited by 44 publications

References 13 publications

Micronucleus test and comet assay on mice fed over five generations a diet containing genetically modified triticale

Micronucleus test and comet assay on mice fed over five generations a diet containing genetically modified triticale

DNA damage and its repair in lymphocytes and thyroid nodule cells during radioiodine therapy in patients with hyperthyroidism

Cytogenetic biomonitoring of Brazilian dentists occupationally exposed to low doses of X-radiation

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