Application of the H³‐proline topographic method to dental tissues

Abstract

Assessment is made in the present study of the applicability of the H3‐proline autoradiographic topographic method to dental and supportive parodontal tissues. Furthermore, comparisons are made of the distribution and rates of matrix production of the mineralized tissues, dentin, cementum and alveolar bone at the maxillary first molars and incisors of 5‐week‐old, short‐lived strain of BNL Swiss albino mice. The results show that tooth collagen matrix production by dentogenic and cementogenic tissues is signicantly larger than by dental or appendicular osteogenic tissues, with incisor dentin formation exceeding all surface activities evaluated to date. It is concluded that the H3‐proline topographic method is a sensitive technique offering greater refinement, and a broader spectrum of analytic possibilities than presently used technology. Integrated cellular and matrical evaluation is achieved, thus adding a new dimension to the assessment of current problems in oral biology.

The distribution of 3H‐proline in alveolar bone of the mouse as seen by radioautography

“…Formation and "migration" of the appositional band in bone are reported by several authors (Stallard, 1963;Crumley, 1964;Garant and Cho, 1979;Dreyer and Sampson, 1984) and are suggested to be useful as a method for determination of bone growth (Kenney and Ramfjord, 1969;Tonna, 1975Tonna, , 1976. This band is likely a direct reflection of the amount of label injected initially (Josephsen and Warshawsky, 1982).…”

“…Osteocytes also produce a small amount of matrix on their lacunar walls (Wassermann and Yaeger, 1965;Baylink and Wergedal, 1971;Jande, 1971Jande, , 1972Baud, 1968;Baud and Auil, 1971; Belanger, 1971, 1973;Zambonin Zallone et al, 1983). Bone is resorbed primarily by osteoclasts and by osteocytes, the latter process being termed osteocytic osteolysis (Belanger, 1969;Baylink et al, 1973;Weisbrode et al, 1974;Parafitt, 1976).Incorporation of 3H-proline into alveolar bone is an important method in the study of the effects of physiologic tooth movement on alveolar bone structure (Stallard, 1963;Crumley, 1964;Baumhammers et al, 1965;Carneiro and Fava de Moraes, 1965;Anderson, 1967;Kenney and Ramfjord, 1969;Tonna, 1975Tonna, ,1976Garant and Cho, 1979;Dreyer and Sampson, 1984). Injection of 3H-proline into a n animal results in creation of a radioactive front resulting from the initial pulse of the label which, in bone, is transferred into a record of labeled collagenous proteins produced by bone synthetic cells (Carneiro and Leblond, 1959;Weinstock and Leblond, 1974;Weinstock, 1975;Leblond and Wright, 1980 ever, labeled bone continues to be formed, likely a direct reflection of the amount of radioactive proline remaining in the body (Josephsen and Warshawsky, 1982).…”

“…The processes of bone formation and resorption have been outlined in some detail by various authors. In the alveolar bone of rodents, it has been well established that new bone is formed on the distal wall and the crest of the interdental septum (Stallard, 1963;Crumley, 1964;Baumhammers et al, 1965;Carneiro and Fava de Moraes, 1965;Anderson, 1967; Kenney and Ramfjord, 1969;Tonna, 1975Tonna, , 1976; Garant and Cho, 1979; Dreyer and Sampson, 1984). Bone resorption occurs on the medial wall of the septum.…”

“…Incorporation of 3H-proline into alveolar bone is an important method in the study of the effects of physiologic tooth movement on alveolar bone structure (Stallard, 1963;Crumley, 1964;Baumhammers et al, 1965;Carneiro and Fava de Moraes, 1965;Anderson, 1967; Kenney and Ramfjord, 1969;Tonna, 1975Tonna, ,1976; Garant and Cho, 1979;Dreyer and Sampson, 1984). Injection of 3H-proline into a n animal results in creation of a radioactive front resulting from the initial pulse of the label which, in bone, is transferred into a record of labeled collagenous proteins produced by bone synthetic cells (Carneiro and Leblond, 1959;Weinstock and Leblond, 1974;Weinstock, 1975; Leblond and Wright, 1980).…”

Cell kinetics of the initial response to orthodontically induced osteogenesis in rat molar periodontal ligament

“…5 region is an area of bone formation. The alveolar crest is forming bone in an occlusal direction [13,14]. As shown in Figure 1, the area of the PDL studied was the mesial (anterior) aspect of the mesial root of the maxillary first molar.…”