Application of the iodine–azide postcolumn reaction in RP‐HPLC for the determination of thioguanine in urine

Abstract

The study focuses on the analysis of a sensitive, selective, and simple postcolumn detection method for thioguanine determination, based on the sensitizing induction of thioguanine on iodine-azide reaction and the combination technique of HPLC. The analysis was accomplished in the optimum conditions for iodine-azide detection system and HPLC separation. The values for the linear range, the LOD, and DOQ amounted to 0.8-1.7, 0.4, and 0.5 nmol/mL urine, respectively.

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

“…Almost all methods [106][107][108] make use of natural structural properties of thiopurines, which possess a well-defined absorption maximum in the ultraviolet spectrum. Recently, Zakrzewski [110] reported a post-column reaction detection method for HPLC determination of thioguanine, based on the sensitizing induction of thioguanine on iodine-azide reaction. The analysis was accomplished in the optimum conditions for iodine-azide detection system and HPLC separation.…”

“…3). The analysis of urine for total thiols had four steps: (1) reduction of disulfides with tris-(2-carboxyethyl)phosphine, (2) cyclization with TCDI, (3) partial purification and (4) separation and quantitation by liquid [110] chromatography with ultraviolet detection. The method is repeatable (variation of less than 5.0%) and sensitive; the limit of detection was 2 pmol per injection.…”

Cytosine derivatized diethylenetriaminepentaacetic acid (dtpa) and Eu(III) complexes for selective recognition of 6-Thioguanine

“…The analysis and detection of 6-TG is considered to be an important task in the field of clinical pathology, which is helpful to not only improve the therapeutic effect of 6-TG, but also know the adverse effects caused by the overdosage in time. Based on the above researches and previous reports [1,7], it could be considered that the Eu III -dtpa-bis(cytosine) complex as a fluorescence probe might be applied to the detection of 6-TG in actual urine. In this experiment, the samples were prepared through adding a certain amount of 6-TG standard solutions and the the corresponding fluorescence spectra were determined in Fig.…”

“…In recent years, it has been applied to treat breast cancer and influence immune response in the treatment of inflammatory diseases such as ulcerative colitis, dermatitis and rheumatoid arthritis [3][4][5][6]. However, the issue of its dosage is of crucial importance, that is, the underdosage will fail in the treatment of the diseases, while relatively adverse effects can be caused by the overdosage, with myelosuppression as the most common [7,8]. Because the safety margin between a therapeutic dose and a toxic dose is very narrow, it is necessary to come up with methods to identify and quantify the drug for forensic medicine and therapeutic drug monitoring.…”

Interaction of glucose‐derived carbon quantum dots with silver and gold nanoparticles and its application for the fluorescence detection of 6‐thioguanine

“…6‐Thioguanine (6‐TG) is a purine compound used as an immunosuppressive agent and also as treatment in the advanced stages of leukemia. To date, several analytical methods have been developed for the detection of 6‐TG, including fluorescence, surface plasmon resonance, high‐performance liquid chromatography, and electrochemical methods . Our group has previously developed a fluorescence turn‐on method based on the interaction of harmine with AgNPs .…”