Application of the rapid lysine decarboxylase test for early isolation and detection of salmonellae in sewage and other wastewaters

Abstract

A method for early isolation and detection of salmonellae in sewage and other wastewaters by using the rapid lysine decarboxylase test as a single biochemical reaction for screening the suspected Salmonella colonies is described. By this method, Salmonella isolation and identification can be completed within 2 to 3 days in contrast to the 5 to 7 days required for the conventional method.

Rapid radiometric method for detection of Salmonella in foods

“…Rapid methods have been developed to reduce the analysis period. These include a specific culture medium (6), biochemical tests (11), and fluorescent-antibody (7) and enrichment serology (2, 5,16,17) techniques. The production of 14CO2 by the bacterial metabolism of 14C-labeled substrates has been used as a rapid screening method for the detection of a wide variety of bacteria in foods (13), water (1), and blood cultures (4).…”

Rapid Detection of Salmonella spp. by Using Felix-O1 Bacteriophage and High-Performance Liquid Chromatography

“…However, unless carefully prepared, non-crossreacting reagents are used, an unacceptably high incidence of false-positive reactions is observed (1,10,15,17,20,21,23,24). On the other hand, methods that utilize growth or metabolic characteristics of a particular microorganism need additional time for the desired traits to be expressed (18,22). Some methods are designed for the identification of characteristic substances elaborated by a particular microorganism (6,13,14,16).…”