Application of the Stern–Volmer equation for studying the spectrofluorimetric quenching reaction of eosin with clindamycin hydrochloride in its pure form and pharmaceutical preparations

Wahba, M. E. K.; El‐Enany, N.; Belal, F.

doi:10.1039/c3ay42093k

Cited by 72 publications

(37 citation statements)

References 12 publications

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“…K sv values were evaluated from the slope of the plot (Geethanjali et al, 2015). Linearity of the plots was tested by calculation of the correlation coefficient (Gong & Zhu, 2013;Wahba, El-Enany & Belal, 2015).…”

Section: Fluorescence Spectroscopic Measurementsmentioning

confidence: 99%

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Ļeonova

Shvirksts

Borisovs

et al. 2020

PeerJ

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1,4-dihydropyridines (1,4-DHP) possess important biochemical and pharmacological properties, including antimutagenic and DNA-binding activity. The latter activity was first described for water-soluble 1,4-DHP with carboxylic group in position 4, the sodium salt of the 1,4-DHP derivative AV-153 among others. Some data show the modification of physicochemical properties and biological activities of organic compounds by metal ions that form the salts. We demonstrated the different affinity to DNA and DNA-protecting capacity of AV-153 salts, depending on the salt-forming ion (Na, K, Li, Rb, Ca, Mg). This study aimed to use different approaches to collate data on the DNA-binding mode of AV-153-Na and five other AV-153 salts. All the AV-153 salts in this study quenched the ethidium bromide and DNA complex fluorescence, which points to an intercalation binding mode. For some of them, the intercalation binding was confirmed using cyclic voltammetry and circular dichroism spectroscopy. It was shown that in vitro all AV-153 salts can interact with four DNA bases. The FTIR spectroscopy data showed the interaction of AV-153 salts with both DNA bases and phosphate groups. A preference for base interaction was observed as the AV-153 salts interacted mostly with G and C bases. However, the highest differences were detected in the spectral region assigned to phosphate groups, which might indicate either conformational changes of DNA molecule (B form to A or H form) or partial denaturation of the molecule. According to the UV/VIS spectroscopy data, the salts also interact with the human telomere repeat, both in guanine quadruplex (G4) and single-stranded form; Na and K salts manifested higher affinity to G4, Li and Rb –to single-stranded DNA.

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Section: Fluorescence Spectroscopic Measurementsmentioning

confidence: 99%

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Ļeonova

Shvirksts

Borisovs

et al. 2020

PeerJ

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“…For clarification of possible binding modes between ET and SC4, thermodynamic parameters such as an enthalpy (ΔH), free energy (ΔG), and entropy (ΔS) data estimated from the following Eqs. 2-4 can be useful [31].…”

Section: Resultsmentioning

confidence: 99%

Intermolecular interactions and binding mechanism of inclusion complexation between sulfonate calix[n]arenes and ethidium bromide

Bayrakcı

Yılmaz

2018

J Incl Phenom Macrocycl Chem

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In this work, the interactions between ethidium bromide (ET) and water-soluble sulfonate calix[n]arenes (n: 4, 6, and 8) were investigated by NMR, FT-IR, and fluorescence spectroscopic methods. The aim was to evaluate both the stoichiometry and the mechanism of the possible complex structure between sulfonate calix[n]arenes and ET. The spectroscopic data revealed that a 1:1 binding mode between calixarene and ET was occurred. Furthermore, thermodynamic parameters and fluorescence titration experiments were studied at different temperatures to determine both the quenching mechanism and the type of intermolecular forces in complex formation. Host-guest complexation of sulfonate calix[n]arenes and ET could be used to overcome some adverse effects related to the using of ethidium bromide during biological applications as a DNA marker treatment.

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“…It is reported that Eosin Y fluorescence is quenched by the development of stable complex with cationic drugs under acidic conditions [29]. Eosin Y has been widely used to develop accurate and precise analytical methods for determination of several compounds of pharmaceutical importance through spectrofluorimetric and/or spectrophotometric measurement [23,27,[30][31][32][33][34][35].…”

Section: Resultsmentioning

confidence: 99%

“…Different analytical techniques were reported for their determination in different matrices including spectrophotometry [3][4][5][6][7][8], spectrofluorimetry [9][10][11], conductometry [12] and HPLC [13][14][15][16][17][18][19][20]. The complex formation that occurs between a lot of drugs and Eosin Y as an ion pairing agent has been considerably investigated using spectrophotometric and spectrofluorimetric assay of such drugs with or without using of metal [21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Validation of Rapid and Sensitive Spectrofluorimetric Assay for Determination of Four Triptans in Pure and Dosage Forms; Application to Human Plasma and Content Uniformity Testing

MA¹,

MA²

2016

Pharm Anal Acta

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A sensitive, simple, rapid and reliable spectrofluorimetric assay was developed for the assay of definite antimigraine drugs namely; Almotriptan malate, Rizatriptan benzoate, Sumatriptan succinate and Zolmitriptan in their pharmaceutical preparations and biological fluid. The suggested procedure was established on determination of the quenching process resulting from the action of the studied drugs on the native fluorescence of Eosin Y via developing of a binary complex reaction between the cited antimigraine preparations and Eosin Y in 0.2 M acetate buffer (pH.3.5). Under the optimized experimental conditions, the relative fluorescence capacity was determined at λ ex =301.3 nm and λ em =542.8 nm. The calibration graphs were linear through extent from 0.07-1.0, 0.20-1.0, 0.2-1.0 and 0.1-1.0 µg/mL, for Almotriptan malate, Rizatriptan benzoate, Sumatriptan succinate and Zolmitriptan, respectively. The detection limits were 0.019, 0.041, 0.055 and 0.032 µg/mL while quantitation limits were 0.059, 0.125, 0.168 and 0.096 µg/mL for Almotriptan malate, Rizatriptan benzoate, Sumatriptan succinate and Zolmitriptan, respectively. The suggested assay has been validated according to ICH and USP guidelines and favorably has been applied to assay of cited drugs in their dosage forms and content uniformity testing. The high sensitivity of the developed assay allowed quantification of the studied antimigraine drugs in human plasma.

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Application of the Stern–Volmer equation for studying the spectrofluorimetric quenching reaction of eosin with clindamycin hydrochloride in its pure form and pharmaceutical preparations

Abstract: A sensitive, selective, economic, and validated spectrofluorimetric method was developed for the determination of clindamycin hydrochloride in pharmaceutical preparations, depending on the reaction of its tertiary amino group with eosin Y.

Cited by 72 publications

References 12 publications

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153

Intermolecular interactions and binding mechanism of inclusion complexation between sulfonate calix[n]arenes and ethidium bromide

Validation of Rapid and Sensitive Spectrofluorimetric Assay for Determination of Four Triptans in Pure and Dosage Forms; Application to Human Plasma and Content Uniformity Testing

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