Application of the Western blotting procedure for the immunodiagnosis of human toxocariasis

Magnaval, Jean-François; Fabre, Richard; Maurières, P; Charlet, Jean-Paul; Larrard, B de

doi:10.1007/bf00928685

Cited by 205 publications

(188 citation statements)

References 21 publications

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“…The MWs of these components are 24,28,30,35,48,56,67,117,136 and 152 kDa. Four of these antigens bands correlate perfectly with LMW bands described by Magnaval et al (1991).…”

Section: Discussionsupporting

confidence: 60%

“…As revealed by western blot, there are two main groups of bands present in the positive cases: one of 24-45 kDa and another group of 66-200 kDa (Magnaval et al 1991, Nunes et al 1997, Morales et al 2002. These studies suggest the HMW bands can cause cross-reactivity and the LMW bands would be more specific for Toxocara.…”

Section: Discussionmentioning

confidence: 75%

“…Western blotting for serodiagnosis of human toxocariasis has also been described (Magnaval et al 1991).…”

mentioning

confidence: 99%

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Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Roldán

Espinoza

2009

Mem. Inst. Oswaldo Cruz

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To improve the serodiagnosis of human toxocariasis, a sensitive and specific enzyme-linked immunoelectrotransfer blot (EITB-IgG) test was developed and evaluated using Toxocara canis larvae excretory-secretory antigens for detecting anti-Toxocara IgG antibodies. The EITB-IgG profile of toxocariasis was characterized by comparing 27 sera from patients with toxocariasis, 110 sera from healthy subjects and 186 sera from patients with other helminth diseases (ascariasis, ancylostomiasis, trichuriasis, enterobiasis, strongyloidiasis, hymenolepiasis, diphyllobothriasis, taeniasis, cysticercosis, hydatidosis and fascioliasis). Antigenic bands of 24, 28, 30, 35, 56, 117, 136

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“…The MWs of these components are 24,28,30,35,48,56,67,117,136 and 152 kDa. Four of these antigens bands correlate perfectly with LMW bands described by Magnaval et al (1991).…”

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 75%

See 1 more Smart Citation

Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Roldán

Espinoza

2009

Mem. Inst. Oswaldo Cruz

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“…MAGNAVAL et al 67 used the WB technique to confirm positivity determined in human sera previously processed by the ELISA technique for IgG antibodies using antigen TES and achieved greater specificity with fractions of low molecular weight (24 to 35 kDa). The study by NUNES et al 78 , using the Immunoblot test, determined that the 55-66 kDa antigenic complex was responsible for the cross reactivity between T. canis and A. suum; whereas LYNCH et al 66 had stated that strong cross reactivity between these two ascarids occurred in the 81 kDa antigen.…”

Section: Immunoblottingmentioning

confidence: 99%

Human toxocariasis: contribution by Brazilian researchers

Chieffi

Santos

Queiroz

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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“…In our laboratory the immunodiagnosis of trichinellosis is performed by using an enzyme-linked immunosorbent assay (ELISA) in which the excretory/secretory (ES) products of Trichinella spiralis muscle larvae are used as antigen (van Knapen et al, 1982 (Magnaval et al, 1991;Gadea et al, 1999). We observed that the IgG4 response directed to a 45 kDa component of ES products of muscle larva was Trichinella-specific.…”

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confidence: 99%

Antigen recognition by IgG4 antibodies in human trichinellosis

et al. 2001

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Summary:The antibody isotype response to Trichinella spiralis excretory/secretory (ES) products of muscle larva was examined using sera from patients with confirmed trichinellosis. Using Western blots we identify components of the ES antigen that are recognized by IgM and IgG antibodies. A 45 kDa component was strongly recognized by different antibody classes and subclasses. We observed a 45 kDa-specific lgG4 response that was detected exclusively using sera of patients with trichinellosis and not of patients with echinococcosis, filariasis, cysticercosis, ascariasis, strongyloidiasis or toxocariasis. These results are relevant for the diagnosis of human trichinellosis.KEYWORDS : human trichinellosis, ES antigen, 45 kDa, lgG4.M onitoring programs for detection of trichinellosis in slaughterhouse animals have dramatically decreased the incidence of human trichinellosis. However, in many parts of the world, including Europe, it continues to occur. Recent outbreaks of trichinellosis have been reported from Germany, France, Spain and Italy (Ancelle et al, 1998;Rodriguez et al., 1999); (Pozio 1998).In our laboratory the immunodiagnosis of trichinellosis is performed by using an enzyme-linked immunosorbent assay (ELISA) in which the excretory/secretory (ES) products of Trichinella spiralis muscle larvae are used as antigen (van Knapen et al., 1982 MATERIALS AND METHODST he excretory/secretory (ES) antigen was prepared using viable muscle larva of T. spiralis as previously described by Gamble (1985). The 45 kDa antigenic component was purified from freshly obtained muscle larva using two-step affinity chromatography as described by Homan et al. (1992) SERUM SAMPLES Serum samples from trichinellosis patients recently received at our laboratory and from an outbreak of trichinellosis that took place in Slupsk, Poland in 1991 were used in this study. The patients here studied were all at an early stage of trichinellosis. All serum samples used tested positive for IgM and/or IgG by means of ELISA using T. spiralis ES antigen. Infection was confirmed for several patients from the outbreak in Poland and for the other patients used in this study by identification of Trichinella larva in muscle biopsy.The source for T. spiralis infection for the outbreak was undercooked pork. Sera from our laboratory from echinococcosis, filariasis, cysticercosis, ascariasis, strongyloidiasis or toxocariasis patients were also included. WESTERN BLOTTING PROCEDUREThe ES antigen or the 45 kDa protein was solubilized under reducing conditions and electrephorized on Parasite, 2001, 8, S168-S171 ANTIGENS S168.

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Application of the Western blotting procedure for the immunodiagnosis of human toxocariasis

Cited by 205 publications

References 21 publications

Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis

Human toxocariasis: contribution by Brazilian researchers

Antigen recognition by IgG4 antibodies in human trichinellosis

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