Application of Thermostable Xylanase of Bacillus pumilus in Textile Processing

Abstract

Desizing of cotton and micropoly fabrics was done using thermostable xylanase from Bacillus pumilus ASH. Micropoly fabric showed better desizing than cotton under same conditions. Violet scale readings from the TEGEWA test after enzymatic desizing for 90 min at pH 7.0 and at 60°C showed the readings falling in the range of 4-5, indicating good desizing efficiency. During bioscouring the weight loss values and liberation of reducing sugars were highest when EDTA was used along with xylanase. The weight loss value of 1.5% was observed for dry cotton fabric after 1 h in case of agitated system at pH 7.0 and at an optimal enzyme dosage of 5 IU/g. The weight loss values and the liberation of reducing sugars were higher in case of cotton fabrics. Wetting time of fabrics was lowered significantly after 60 min of bioscouring using xylanase. Increase in temperature or concentration of surfactant led to further reduction in the wetting time. The whiteness values of fabrics after bioscouring were 0.9% higher than the chemically scoured fabrics indicating good efficacy of xylanase during the scouring process.

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

“…Further, it also helped in decreasing in yellowness of fabric by 5.57%. Similarly, xylanase from Bacillus pumilus was studied for enzymatic desizing of cotton and micropoly fabrics (Battan et al 2012). The enzymatic desizing with enzyme load of 5 IU/g at pH 7.0, temperature 60 °C for 90 min resulted in improved whiteness of 0.9% with respect to the chemical process.…”

“…The enzymatic desizing with enzyme load of 5 IU/g at pH 7.0, temperature 60 °C for 90 min resulted in improved whiteness of 0.9% with respect to the chemical process. The addition of surfactant such as EDTA improved the desizing and bioscouring efficiency (Losonczi et al 2005;Battan et al 2012;Garg et al 2013).…”

Combined de sizing and scouring of cotton fabric using xylanase enzyme

“…Increase in reaction time and agitation also results in increase in weight loss percentage of the fabric. 18 It is also observed that the weight loss percentage of cotton is higher initially in the samples treated at 2g/l enzyme concentration. Further it is also found that the wetting time values of fabrics obtained after 90min of treatment were significantly lower to that of fabrics obtained after 60min of treatment whereas further increasing the treatment time from 60 to 120min did not result in much lowering of the wetting time.…”

Thermostable xylanase from thermophilic fungi: Biochemical properties and industrial applications

“…They are additives in the formulation of detergents and, thus, improve the removal of stains of vegetable origin (Kamal et al, 2004). In the textile, enzymes act on cotton cleaning (cotton biopolishing), in order to improve the physical characteristics of the tissue, to assist in the removal of non-cellulosic materials and facilitate alkaline extraction step to improve the access of compounds chemicals to the fiber, leading to a reduction in consumption of these agents and possible environmental damage (Battan et al, 2012;Csiszár et al, 2006).…”