Application of Tissue Culture Techniques to Improve the Productivity of Medicinal Secondary Products from Medicinal Plants

Hassanein, A. M.

doi:10.5772/intechopen.105193

Cited by 3 publications

(5 citation statements)

References 158 publications

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“…Gels were run at 10 mA per gel for 6 h at 4 ºC with 0.025 M Tris-HCl + 0.192 M glycine buffer, pH 8.9. The peroxidase bands Citation: Hassanein, A.M.;Salem, J.M;El-Deeb, B.A. ;Farghal, Z.…”

Section: Peroxidase Patternmentioning

confidence: 99%

“…For conventional banana propagation, corms, large and small suckers, and sward suckers are used (Arias, 1992) but these plant materials carry weevils, fungal pathogens, nematodes and viruses (Ganapathi et al, 2001). Therefore, plant tissue culture techniques are recommended to obtain multiplication in high quality, disease-free and uniform plants irrespective of the season (Hassanein, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…* Means that differences were significant at p < 0.05, compared to control (0 day).Citation:Hassanein, A.M.;Salem, J.M;El-Deeb, B.A. ;Farghal, Z.…”

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confidence: 99%

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Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions

Saad,

Hassanein,

El-Deep

et al. 2023

Journal of Environmental Studies

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Transfer banana plantlet from in vitro to ex vitro conditions is essential prerequisite in micropropagation. In vitro grown plantlets are unable to cope with soil environment. To avoid these challenges, the plant must be acclimatized according to a protocol that takes into account the economics of the plant concerned. Banana shoot culture was established on MS medium supplemented with 5 mg/l BAP and 30 g/l sucrose. Shoot cuttings were subjected for in vitro root formation on half strength MS medium containing 1 mg/l IBA. Plantlets were acclimatized under tissue culture room conditions where they were able to repair their physiological disorders and cope ex vitro conditions. Disorder repair processes were better if plantlets were treated with chitosan. When chitosan was used, efficiency of acclimatization increased during all acclimatization periods due to the increase in plant growth parameters, water content and ROS scavenging through the increase in activities of antioxidant enzymes such as superoxide dismutase and peroxidase. To save time and costs, root formation and acclimatization were combined in one step under transparent plastic bags (relative humidity 87%) in tissue culture room (25 o C) using ex vitro-hydroponic or soil-root formation procedure. Survival frequency was 100% when root formation was induced using 1 mg/l IBA and relative humidity was gradually decreased. Plantlets were transplanted in soil without application of in vitro or ex vitro root formation procedures died in five days.

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Section: Peroxidase Patternmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions

Saad,

Hassanein,

El-Deep

et al. 2023

Journal of Environmental Studies

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“…The latest developments in plant in vitro techniques have led to a successful transition from the experimental stage to industrial-scale production. Cultivation of plant differentiated in vitro systems from last year is believed to be an effective method for generating valuable natural compounds (Hassanein, 2022). Numerous research studies have investigated the effect of nitrogen concentration in cultivation media on the growth and morphogenesis of tissue cultures.…”

Section: Literature Reviewmentioning

confidence: 99%

Impact of nutrient supply on growth and synthesis of metabolites of in vitro shoot cultures of S. rebaudiana

Smetanska,

Diaa,

Nazim

et al. 2023

Rosl. g̀runtozn.

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Stevia rebaudiana plants produce sweet tasting compounds, steviosides, which are 300 times sweeter as sugar and can be used as natural sweeteners. Moreover, stevia leaves contain high amount of antioxidants. The use of plant compounds as components of functional nutrition and nutraceuticals is extremely relevant today. The purpose of this study was to investigate the effect of increased nitrogen and saccharose supply on growth and accumulation of steviosides and anthocyanins as well as antioxidative activity in fast-growing shoot cultures of S. rebaudiana. The morphological changes of the leaves, the accumulation of steviosides and anthocyanins, and the antioxidant properties of the extracts were determined. It was shown that during three weeks of in vitro cultivation of shoots on MS medium with double concentration of nitrogen, the biomass increased by 71.0%, after application of double concentration of saccharose – by 133%, and after increasing nitrogen in combination with saccharose supply – by 162.0% compared to the control. Sucrose stimulated the accumulation of biomass. Anti-oxidative potential after nitrogen application was 2.4 times higher than the control, and with saccharose supply it increased 2.7 times. Shoots treated with increased nitrogen and saccharose concentration contained 50.7 and 57.8 mg/g-1 steviosides, respectively. However, the combination of nitrogen and saccharose led to accumulation of 73.4 1 mg/g-1 steviosides. Shoots grown on MS medium culture had 26.0 mg/g-1 of steviosides. The content of anthocyanins was 1.7 times greater under added nitrogen supply, and 2.3 times greater after the application of nitrogen and saccharose. Optimum cultivation media developed individually for each in vitro culture increase the production of valuable plant secondary metabolites up to 3 times

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Section: Literature Reviewmentioning

confidence: 99%

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2023

Rosl. g̀runtozn.

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samples were analysed for humus content according to DSTU 4289:2004 and labile organic matter content according to DSTU 4732:2007. When chernozems are ploughed up, labile organic matter is mineralised the most and their share in humus decreases, while when arable land is converted to fallow land, it is restored the fastest. Minimising tillage systems and applying organic and mineral fertilisers increases the amount of labile organic matter in the humus of chernozems. The use of notill for sixteen years resulted in a 0.13% increase in humus content, and a 0.53% increase over fortytwo years compared to ploughing. The application of organo-mineral fertiliser increased the ratio of labile organic matter carbon content to total carbon content compared to the unfertilised variants. A strong correlation was found between the content of labile organic matter in chernozems and the yield of winter wheat and maize. The practical significance of the research results is to minimise the fertiliser rate and the C:N ratio to ensure gross crop yields and restore soil fertility

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Application of Tissue Culture Techniques to Improve the Productivity of Medicinal Secondary Products from Medicinal Plants

Cited by 3 publications

References 158 publications

Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions

Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions

Impact of nutrient supply on growth and synthesis of metabolites of in vitro shoot cultures of S. rebaudiana

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