Application of toxicity monitor using nitrifying bacteria biosensor to sewerage systems

Inui, Takashi; Tanaka, Yoshiaki; Okayasu, Yuji; Tanaka, Hiroaki

doi:10.2166/wst.2002.0603

Cited by 16 publications

(15 citation statements)

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“…Also, whole-cell biosensors for environmental monitoring or detection of pollutants in wastewaters have been reported (Rawson et al, 1989;Rois et al, 1984). A variety of microbial sensors for application to wastewater analysis have been developed up to recent study (Choi and Gu, 2002;Farre and Barcelo, 2001;Horsburgh et al, 2002;Inui et al, 2002;Rastogi et al, 2003;Tanaka et al, 2002). A whole-cell microbial sensor consisting of Nitrosomonas europaea and an oxygen electrode for the detection of cyanide have been described elsewhere (Nakamura et al, 1990;Shinohara and Tanaka 1989).…”

Section: Introductionmentioning

confidence: 99%

Development of an automated water toxicity biosensor using Thiobacillus ferrooxidans for monitoring cyanides in natural water for a water filtering plant

Okochi¹,

Mima²,

Miyata³

et al. 2004

Biotech & Bioengineering

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An on-line biosensor consisting of immobilized Thiobacillus ferrooxidans and an oxygen electrode was developed for automated monitoring of acute toxicity in water samples. T. ferrooxidans is an obligatory acidophilic, autotrophic bacterium and derives its energy by the oxidation of ferrous ion, elemental sulfur, and reduced sulfur compounds including metal sulfides. The assay is based on the monitoring of a current increase by addition of toxicoids, which is caused by the inhibition of bacterial respiration and decrease in oxygen consumption. Optimum cell number on the membrane was 5.0 x 10(8) cells. The steady-state current was obtained when concentration of FeSO4 was above 3.6 mM at pH 3. The sensor response of T. ferrooxidans immobilized membrane for 5.0 microM KCN was within an error of 10% for 30 membranes. A linear relationship was obtained at KCN concentration in the range of 0.5-3.0 microM in a flow-type monitoring system. Minimum detectable concentrations of KCN, Na2S, and NaN3 were 0.5, 1.2, and 0.07 microM, respectively. The monitoring system contained two biosensors and these sensors were cleaned with sulfuric acid (pH 1.5) twice a day. This treatment could remove fouling on microbial immobilized membrane by natural water and ferrous precipitation in the flow cell. This flow-type monitoring sensor was operated continuously for 5 months. Also, T. ferrooxidans immobilized membrane can be stored for one month at 4 degrees C when preserved with wet absorbent cotton under argon gas.

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Section: Introductionmentioning

confidence: 99%

Development of an automated water toxicity biosensor using Thiobacillus ferrooxidans for monitoring cyanides in natural water for a water filtering plant

Okochi¹,

Mima²,

Miyata³

et al. 2004

Biotech & Bioengineering

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“…The most popular approach uses cells with an introduced luminescent reporter geneto determine changes in the metabolic status of the cells following intoxication (Bentley et al, 2003).Nitrifying bacteria have multiple-folded cell membranes, which are sensitive to all membrane disintegrating substances: organic solvents, surfactants, heavy metals, and oxidants. Therefore, respirometric sensors measuring the respiration rates of these bacteria can be used for toxicity monitoring in wastewater treatment (Inui et al, 2002).…”

Section: Biomonitoringof Bioremediation: Biosensorsmentioning

confidence: 99%

Microbial Bioremediation of some Heavy Metals in Soils: An updated review

Girma¹

2015

Egyptian Academic Journal of Biological Sciences, G. Microbiolo

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Nowadays, due to industrialization and extraction of natural resources, soil and water pollution is one of the major global concerns. During the recent era of environmental protection, the use of microorganisms for the recovery of heavy metals from soil, sediments and water as well as employment of plants for landfill applications has generated growing attention. The role of microorganisms and plants in biotransformation of heavy metals into nontoxic forms is well-documented, and understanding the molecular mechanism of metal accumulation has numerous biotechnological implications for bioremediation of metal-contaminated sites. The food and water we consume are often contaminated with a range of chemicals and heavy metals, such as gold, copper, nickel, zinc, lead, cadmium, arsenic, chromium, and mercury that are associated with numerous diseases. Human activities like metalliferous mining and smelting, agriculture, waste disposal or industry discharge these metals which can produce harmful effects on human health when they are taken up in amounts that cannot be processed by the organism. Many studies have demonstrated that microbes have the ability to remove heavy metals from contaminated soils. Among others some of the microorganisms that play great role in bioremediation of heavy metals are Pseudomonas spp.

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“…Nitrifying bacteria have multiple-folded cell membranes, which are sensitive to all membranedisintegrating substances: organic solvents, surfactants, heavy metals, and oxidants. Therefore, respirometric sensors measuring the respiration rates of these bacteria can be used for toxicity monitoring in wastewater treatment (31). Biosensors measuring concentrations of hazardous substances are often based on the measurement of bioluminescence (32).…”

Section: Biosensorsmentioning

confidence: 99%

Applications of Environmental Biotechnology

Ivanov

Hung

2010

Environmental Biotechnology

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Environmental biotechnology is a system of scientific and engineering knowledge related to the use of microorganisms and their products in the prevention of environmental pollution through biotreatment of solid, liquid, and gaseous wastes, bioremediation of polluted environments, and biomonitoring of environment and treatment processes. The advantages of biotechnological treatment of wastes are as follows: biodegradation or detoxication of a wide spectrum of hazardous substances by natural microorganisms; availability of a wide range of biotechnological methods for complete destruction of hazardous wastes; and diversity of the conditions suitable for biodegradation. The main considerations for application of biotechnology in waste treatment are technically and economically reasonable rate of biodegradability or detoxication of substances during biotechnological treatment, big volume of treated wastes, and ability of natural microorganisms to degrade substances. Type of biotreatment is based on physiological type of applied microorganisms, such as fermenting anaerobic, anaerobically respiring (anoxic), microaerophilic, and aerobically respiring microorganisms. All types of biotechnological treatment of wastes can be enhanced using optimal environmental factors, better availability of contaminants and nutrients, or addition of selected strain(s) biomass. Bioaugmentation can accelerate start-up or biotreatment process in case microorganisms, which are necessary for hazardous waste treatment, are absent or their concentration is low in the waste; if the rate of bioremediation performed by indigenous microorganisms -T. Hungis not sufficient to achieve the treatment goal within the prescribed duration; when it is necessary to direct the biodegradation to the best pathway of many possible pathways; and to prevent growth and dispersion in waste treatment system of unwanted or nondetermined microbial strain which may be pathogenic or opportunistic one. Biosensors are essential tools in biomonitoring of environment and treatment processes. Combinations of biosensors in array can be used to measure concentration or toxicity of a set of hazardous substances. Microarrays for simultaneous qualitative or quantitative detection of different microorganisms or specific genes in the environmental sample are also useful in the monitoring of environment.Key Words Environmental biotechnology r wastes r biotreatment r biodegradation r bioaugmentation r biosensors r biomonitoring.

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Application of toxicity monitor using nitrifying bacteria biosensor to sewerage systems

Cited by 16 publications

References 0 publications

Development of an automated water toxicity biosensor using Thiobacillus ferrooxidans for monitoring cyanides in natural water for a water filtering plant

Development of an automated water toxicity biosensor using Thiobacillus ferrooxidans for monitoring cyanides in natural water for a water filtering plant

Microbial Bioremediation of some Heavy Metals in Soils: An updated review

Applications of Environmental Biotechnology

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