Application of two different synthetic sequential media for the human IVF-ET program: a prospective, randomized, and comparative study

Abstract

ObjectiveSince IVF program was first established, various types of media and culture systems have been developed either in-house or commercially. The aim of this study was to compare the efficacy of in-house Maria Research Center (MRC) media to that of commercially available Sydney IVF media in human day 3 embryo transfer cycles.MethodsThree hundred sixty nine couples were included in this prospective, randomized, and comparative study. All couples undergoing IVF treatment at the Maria Fertility Hospital were randomly assigned to either Sydney IVF (n=178) or MRC (n=191) media.ResultsNo difference was observed between the MRC media and Sydney IVF media groups with respect to fertilization rate (74.4% vs. 75.5%). The clinical pregnancy and implantation rates of MRC media (47.1% and 20.0%, respectively) were also similar to those of Sydney IVF media (44.4% and 19.4%, respectively). However, the proportion of embryos with good quality on day 3 was significantly higher in the MRC media group than the Sydney IVF media group (50.2% vs. 43.2%) (p<0.05).ConclusionMRC media were as effective as Sydney IVF media for sustaining embryo development and pregnancy rates. The present study implies that MRC media can be a suitable alternative to commercially available media for human IVF-ET program.

Single versus sequential culture medium: which is better at improving ongoing pregnancy rates? A systematic review and meta-analysis

“…Embryo culture is an important step in ART, and culture medium plays a key role in the success of IVF cycles ( Yoon et al. , 2011 ; Khoury et al.…”

Analysis of clinical outcomes with respect to spermatozoan origin after artificial oocyte activation with a calcium ionophore

“…The samples were examined 17-19 h after insemination for the presence and number of pronuclei (PN), which indicate fertilization. Over the fiveyear study period, three different culture media were used: YS [24], Cook media (Brisbane, Australia), and MRC [25]. Three days after oocyte retrieval and insemination, the embryos were transferred in the uterus of the female patient.…”

Effects of resveratrol, granulocyte-macrophage colony-stimulating factor or dichloroacetic acid in the culture media on embryonic development and pregnancy rates in aged mice

“…Each female mouse was given an intraperitoneal injection of pregnant mare serum gonadotropin (PMSG; Folligon, Intervet, UK) followed by an injection of human chorionic gonadotropin (hCG; Chorulon, Intervet, UK) 48 h later. The mice were euthanized by cervical dislocation and the metaphase II (MII) oocytes were collected 14 h post-hCG injection in pre-warmed MRC#D01 medium (Maria Medical Foundation, South Korea) as described previously [40].…”