Application of UPLC- ESI-q-TOF analysis for screening of the carcinogen-modified DNA-adducts in the circulation DNA of patients with lung cancer

Abstract

Abstract. In this paper we have presented the development of a new approach to screening a carcinogen associated DNA adducts. As starting-material circulating DNA from blood of patients with a diagnosis of lung cancer was used. To obtain DNA adducts DNA of 100 patients and 100 healthy volunteers was hydrolyzed with 0.1 M hydrochloric acid under heating and then neutralized. The LC-MS analysis was performed on a UPLC-ESI-q-TOF mass spectrometry system with using C-18 column. Mass spectra were recorded in positive mode with 50-1000 m/z range. Simultaneously 7 DNA adducts were identified by using the approach described in this article.

DNA adducts: Formation, biological effects, and new biospecimens for mass spectrometric measurements in humans

“…However, Q‐TOF was reported to have limited dynamic range and reduced sensitivity towards low concentrations of small molecular analytes and peptides compared to QqQ . Only a few studies have used Q‐TOF instrument for DNA adduct analysis …”