Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form

Sonawane, Sandeep; Shirkhedkar, Atul A.; Fursule, Ravindra A.; Surana, Sanjay J.

doi:10.12973/ejac/77004

Cited by 32 publications

(24 citation statements)

References 7 publications

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“…Atorvastatin calcium {[R-(R, R*)]-2-(4-flurophenyl)-β,δ-dihydroxy-5(1-methylethyl)-3-phenyl-4-[phenylamino)carbonyl]-1H-pyrrole-1-heptanoic acid, calcium salt (2:1) trihydrate} is the most commonly occurring drug in commercially available pharmaceutical formulations used for the clinical treatment of hypercholesterolemia [1]. Several methods have been described for the quantitative determination of atorvastatin by high-performance liquid chromatography (HPLC) in different pharmaceutical preparations, either alone [2][3][4][5][6][7] or with other active ingredients [8][9][10][11][12][13][14][15][16]. HPTLC [17], electrochemical [18,19], capillary electrophoresis [20] and spectrofluorimetric [21] methods have been developed for the analysis of atorvastatin from its individual and combined formulations with other active ingredients in pharmaceutical preparations and plasma.…”

Section: Introductionmentioning

confidence: 99%

New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

Ashour¹

2013

Pharmaceut Anal Acta

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New, accurate, sensitive and reliable kinetic spectrophotometric method for the assay of atorvastatin calcium (AVS) in pure form and pharmaceutical formulations has been developed. The method involves the oxidative coupling reaction of AVS with 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) in the presence of Ce(IV) in an acidic medium to form colored product with lambdamax at 566 nm. The reaction is followed spectrophotometrically by measuring the increase in absorbance at 566 nm as a function of time. The initial rate and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 2.0-20.0 µg/mL for initial rate and fixed time methods. The limit of detection for initial rate and fixed time methods is 0.093 and 0.064 µg/mL, respectively. Molar absorptivity for the method was found to be 3.36×10 4 L/ mol cm. Statistical treatment of the experimental results indicates that the methods are precise and accurate. Both methods have been applied successfully for the estimation of pravastatin sodium in commercial dosage forms with no interference from the excipients. The results are compared with the pharmacopoeial method.

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Section: Introductionmentioning

confidence: 99%

New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

Ashour¹

2013

Pharmaceut Anal Acta

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“…Several methods have already been reported concerning the determination of atorvastatin in pharmaceutical formulations and clinical samples, including the HPLC system, [5][6][7][8][9][10][11][12] spectrophotometry, 13,14 micellar liquid chromatographic, 15 densitometry 16 and reverse-phase HPLC. 17 Electrochemical techniques, such as differential pulse voltammetry (DPV) using modified or unmodified electrodes, can be considered for the determinations of pharmaceutical compounds as strong alternatives to the other instrumental methods.…”

Section: Introductionmentioning

confidence: 99%

“…The proposed method is highly selective, and more sensitive vs. the reported electrochemical methods for the determination of atorvastatin in real samples at ultra trace levels. 8,9,[11][12][13][14][15][16][17][39][40][41] …”

Section: Introductionmentioning

confidence: 99%

Polytetrafluorethylene Film-Based Liquid-Three Phase Micro Extraction Coupled with Differential Pulse Voltammetry for The Determination of Atorvastatin Calcium

2013

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In this paper, we describe a new combination method based on polytetrafluorethylene (PTFE) film-based liquid threephase micro extraction coupled with differential pulse voltammetry (DPV) for the micro extraction and quantification of atorvastatin calcium (ATC) at the ultra-trace level. Different factors affecting the liquid-three phases micro extraction of atorvastatin calcium, including organic solvent, pH of the donor and acceptor phases, concentration of salt, extraction time, stirring rate and electrochemical factors, were investigated, and the optimal extraction conditions were established. The final stable signal was achieved after a 50 min extraction time, which was used for analytical applications. An enrichment factor of 21 was achieved, and the relative standard deviation (RSD) of the method was 4.5% (n = 4). Differential pulse voltammetry exhibited two wide linear dynamic ranges of 20.0 -1000.0 pmol L -1 and 0.001 -11.0 μmol L -1 of ATC. The detection limit was found to be 8.1 pmol L -1 ATC. Finally, the proposed method was used as a new combination method for the determination of atorvastatin calcium in real samples, such as human urine and plasma.

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“…A variety of analytical methods are reported such as, estimation of enantiomeric of ATOR-C 3 , in human serum 4 and its impurity in bulk drugs 5 . The majority of methods reported are liquid chromatography in which ATOR-Cwas estimated simultaneously with ezetimibe 1,6,7 , fenofibrate 8 , aspirin 9,10 , ramipril 11,12 , nicotinic acid 13 and amLodipine 14,15,16,17,18 . Some stability indicating RP-HPLC methods of ATOR-C and Amlodipine 19 was also reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of Atorvastatine in Pharmaceutical Formulations by Reverse Phase-High Performance Liquid Chromatography

Charde¹,

Gupta²,

Chakole³

2012

Int J of Adv in Phar Ana

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A simple, sensitive and reproducible reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitative estimation of Atorvastatin calcium (ATOR-C) in the pharmaceutical formulations. Chromatographic separation was achieved on a 250 × 4.6 mm, 5µ, Waters symmetry column. The flow rate was 1mL/min and eluent was monitored by absorbance at 246.0 nm using a mixture of Methanol and Acetonitrile (pH 3.0±0.01) in the ratio of 25:75 (v/v). The retention times of ATOR-C was found to be 5.5 min. Calibration plots were linear in the concentration range of 5-25 µg/mL for ATOR-C calcium. The total run time was 12 min. The proposed method was validated by testing its linearity, recovery, specificity, system suitability, precision (Interday, intraday, analyst and instrument precision), robustness and LOD/LOQ values and it was successfully employed for the determination of ATOR-C in pharmaceutical tablet formulations.

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Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form

Cited by 32 publications

References 7 publications

New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

Polytetrafluorethylene Film-Based Liquid-Three Phase Micro Extraction Coupled with Differential Pulse Voltammetry for The Determination of Atorvastatin Calcium

Determination of Atorvastatine in Pharmaceutical Formulations by Reverse Phase-High Performance Liquid Chromatography

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