Application of UV-Vis spectrophotometric and chemiluminescent methods for evaluation of the antioxidant action of curcumin

Abstract

Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5- dione) is a natural biological active substance with an antioxidant activity. The ability of curcumin to inhibit the free radical mechanisms can be used in a prevention of diseases such as cancer and coronary heart disease. The UV-VIS spectrophotometric and chemiluminescent dynamic methods for determination of antioxidant activity of curcumin were developed. The spectrophotometric method includes investigation of the interaction between DNA, isolated from HL-60 cells, and curcumin. The decreasing of the absorption of curcumin in the presence of HL-60 DNA against the blank sample can be a measurement for some complex formation between curcumin and DNA. The chemiluminescent method involves three tests for detection of luminol - depending chemiluminescence on the base of model systems which generate superoxide, hydroxide and hypochlorite radicals. The strongest decay of chemilunimescence was registered at the highest concentration of curcumin (100 μmol/L)

The electrochemical characterization of curcumin and its selective detection in Curcuma using a graphene-modified electrode

“…10,11 In the literature, some methods for the determination of curcumin are reported, such as high-performance liquid chromatography (HPLC), [12][13][14] spectrouorimetry, 15,16 and highperformance thin-layer chromatography. 17 Besides, UV-vis spectrophotometric and chemiluminescent dynamic methods 18 were developed for investigating the antioxidant activity of curcumin. Comparing these, the electroanalytical technique has the advantages of simplicity and high sensitivity, and simultaneously can give some information about the reaction mechanism between medical and biological molecules.…”

Dyeing Ramie Fabrics With Curcumin in NaOH/Urea Solution at Low Temperature

“…to its simple extraction (Asche & Thakkar, 2004;Mandal, Maity, Dewanjee, & Mandal, 2008;Pyo & Kim, 2014;Vrchlabsky, 1972), antioxidant properties (Sharma, 1976;Stancho, Ivanka, Spiro, & Danka, 2012), anti-inflammatory properties (Dahl, McGowan, Shand, & Srinivasan, 1989;Yi & Yoo, 2010), and anti-ultraviolet (UV) properties (Zhou & Tang, 2016). Curcumin, a bright yellow material, is sold as an herbal tea, cosmetic ingredient, food flavoring, and textile coloring.…”

“…In recent years, there has been growing interest in the revival of natural dyestuffs from plants, such as rubican, carthamin, hematin, gardenia yellow pigment, laccaic acid, tea pigment, and curcumin (Boonroeng, Srikulkit, Xin, & He, 2015; Zhou, Zhang, Tang, & Zhang, 2015). In this work, curcumin was chosen as a representative source for a plant-based yellow natural dye due to its simple extraction (Asche & Thakkar, 2004; Mandal, Maity, Dewanjee, & Mandal, 2008; Pyo & Kim, 2014; Vrchlabsky, 1972), antioxidant properties (Sharma, 1976; Stancho, Ivanka, Spiro, & Danka, 2012), anti-inflammatory properties (Dahl, McGowan, Shand, & Srinivasan, 1989; Yi & Yoo, 2010), and anti-ultraviolet (UV) properties (Zhou & Tang, 2016). Curcumin, a bright yellow material, is sold as an herbal tea, cosmetic ingredient, food flavoring, and textile coloring.…”