Application of Vapor Phase Chromatography to Mass Spectrometer Analysis

Drew, Charles M.; McNesby, J. R.; Smith, S. Ruven; Gordon, Alvin S.

doi:10.1021/ac60114a015

Cited by 40 publications

(13 citation statements)

References 18 publications

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“…Griffiths, James, and Phillips (10) first applied PTGC to the elution of organic halides. Subsequent workers have reported on the apparatus requirements (2, 8, 12), and on the application of PTGC to separations using the gassolid and gas-liquid elution techniques (1,(5)(6)(7)(8)(9)16). A case in which nonlinear programming was used was reported recently (17).…”

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confidence: 99%

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Programmed Temperature Gas Chromatography

Nogare¹,

Langlois²

1960

Anal. Chem.

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confidence: 99%

“…In particular, the inlet pressure p¡ is obtained by setting x = 0, whence Pi = po Vl + (2JMT/p<,2) (6) Because this can be rewritten as J = (Pi2 -po2)/2MT (7) an easy means of determining the dependence of J on temperature from a series of p¡ observations at different values of M and T is available.…”

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confidence: 99%

Programmed Temperature Gas Chromatography

Nogare¹,

Langlois²

1960

Anal. Chem.

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“…Historically, the development of GC for separation and detection by MS went hand-in-hand (see Chapter 3) (Biemann and Vetter, 1960). After the initial publications on GC in 1952 , the first successful application of analytical MS was already documented in 1956 by Drew who described the separation and subsequent analysis of hexane, propyne (methylacetylene), propylene and allene isomers (Drew et al, 1956). State-of-the-art GC-MS is mainly based on either electron -25 -ionization (EI) or chemical ionization (CI).…”

Section: Aad Technologies -An Overviewmentioning

confidence: 99%

The why and how of amino acid analytics in cancer diagnostics and therapy

Manig

Kuhne

Neubeck

et al. 2017

Journal of Biotechnology

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Pathological alterations in cell functions are frequently accompanied by metabolic reprogramming including modifications in amino acid metabolism. Amino acid detection is thus integral to the diagnosis of many hereditary metabolic diseases. The development of malignant diseases as metabolic disorders comes along with a complex dysregulation of genetic and epigenetic factors affecting metabolic enzymes. Cancer cells might transiently or permanently become auxotrophic for non-essential or semi-essential amino acids such as asparagine or arginine. Also, transformed cells are often more susceptible to local shortage of essential amino acids such as methionine than normal tissues. This offers new points of attacking unique metabolic features in cancer cells. To better understand these processes, highly sensitive methods for amino acid detection and quantification are required. Our review summarizes the main methodologies for amino acid detection with a particular focus on applications in biomedicine and cancer, provides a historical overview of the methodological pre-requisites in amino acid analytics. We compare classical and modern approaches such as the combination of gas chromatography and liquid chromatography with mass spectrometry (GC-MS/LC-MS). The latter is increasingly applied in clinical routine. We therefore illustrate an LC-MS workflow for analyzing arginine and methionine as well as their precursors and analogs in biological material. Pitfalls during protocol development are discussed, but LC-MS emerges as a reliable and sensitive tool for the detection of amino acids in biological matrices. Quantification is challenging, but of particular interest in cancer research as targeting arginine and methionine turnover in cancer cells represent novel treatment strategies.

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“…The closest competitor to mass spectrometry as an analytical tool is probably vapor phase chromatography (8), which is considerably cheaper and simpler, but also considerably less versatile. A combination of the two techniques is possible where the eluate gas from a vapor phase chromatogram is introduced in a mass spectrometer (9).…”

Section: Quantitative Analysismentioning

confidence: 99%