Application of Viral Metagenomics for Study of Emerging and Reemerging Tick-Borne Viruses

Damian, Donath; Maghembe, Reuben; Damas, Modester; Wensman, Jonas Johansson; Berg, Mikael

doi:10.1089/vbz.2019.2579

Cited by 16 publications

(19 citation statements)

References 45 publications

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“…Hard ticks were collected from the body of domestic animals (cattle and goats) and from the environment (freeliving ticks) [7]. Ticks collection was done from April to July 2019 in the wards which lie at the border of Mikumi National park, Morogoro Region, Tanzania (figure 1).…”

Section: Sample Collectionmentioning

confidence: 99%

“…Adult ticks were collected by plucking using blunt forceps from domestic animals. Whereas, the questing (free-living) ticks were collected from purposefully chosen areas within logistical constraints, and include host resting areas and burrows, host routes and areas surrounding watering holes [7]. After collection, all ticks were transported to the laboratory at the Department of Parasitology and Entomology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, Tanzania.…”

Section: Sample Collectionmentioning

confidence: 99%

“…20 ticks per pool were homogenized with a mortar and pestle in DNase buffer [7]. The homogenates were centrifuged at 10,000×g for 10 minutes at 4°C.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Identification of tick viruses is essential to the control of tick-borne diseases. Since many viruses cannot be cultivated and lack common viral gene markers, the detection of viruses using conventional, immunological, and PCR methods is very difficult [7]. Furthermore, many as yet undiscovered viruses cannot be identified using these specific methods.…”

Section: Introductionmentioning

confidence: 99%

“…This study focused on viruses of ticks in the wildlifelivestock interface ecosystem of Mikumi National Park, Morogoro region, Tanzania based on metagenomic as a powerful tool to investigate emerging and re-emerging viruses [7]. To our knowledge, this is the first report from Tanzania in which ticks viruses are quantified using metagenomic techniques.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Viruses in Hard Ticks (Ixodidae) from Select Areas of a Wildlife-livestock Interface Ecosystem at Mikumi National Park, Tanzania

Damian¹,

Damas²,

Wensman³

et al. 2020

AJBIO

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Many of the recent emerging infectious diseases have occurred due to the transmission of the viruses that have wildlife reservoirs. Arthropods, such as ticks, are known to be important vectors for spreading viruses and other pathogens from wildlife to domestic animals and humans. In the present study, we explored the diversity of viruses in hard ticks (Ixodidae) from select areas of a wildlife-livestock interface ecosystem at Mikumi National Park, Tanzania using a metagenomic approach. cDNA and DNA were amplified with random amplification and Illumina high-throughput sequencing was performed. The high-throughput sequenced data was imported to the CLC genomic workbench and trimmed based on quality (Q = 20) and length (≥ 50). The trimmed reads were assembled and annotated through Blastx using Diamond against the National Center for Biotechnology Information non-redundant database and its viral database. The MEGAN Community was used to analyze and to compare the taxonomy of the viral community. The obtained contigs and singletons were further subjected to alignment and mapping against reference sequences. The viral sequences identified were classified into bacteria, vertebrates, and invertebrates, plants, and protozoans viruses. Sequences related to known viral families; Retroviridae,

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Section: Sample Collectionmentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

“…20 ticks per pool were homogenized with a mortar and pestle in DNase buffer [7]. The homogenates were centrifuged at 10,000×g for 10 minutes at 4°C.…”

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Diversity of Viruses in Hard Ticks (Ixodidae) from Select Areas of a Wildlife-livestock Interface Ecosystem at Mikumi National Park, Tanzania

Damian¹,

Damas²,

Wensman³

et al. 2020

AJBIO

Self Cite

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Molecular evidence for potential transovarial transmission of Jingmen tick virus in Haemaphysalis longicornis fed on cattle from Yunnan Province, China

Chen

Zhang³

et al. 2022

Journal of Medical Virology

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Jingmen tick virus (JMTV) is a novel tick‐borne virus first identified from Jingmen city, Hubei Province of China in 2010. It has been proved that JMTV can cause human diseases and is widely distributed both inside and outside of China. However, the survival mode and transmission characteristics of JMTV still need further research, particularly in terms of transovarial transmission. In this study, an investigation was conducted to explore the presence of JMTV from engorged female ticks to their offspring. All engorged female adult ticks were collected from domestic cattle and allowed to lay eggs in appropriate humidity and temperature conditions. Maternal ticks, eggs and larvae were screened for JMTV RNA through real‐time polymerase chain reaction (RT‐PCR) and nested PCR methods. The results revealed the positive rate of 10.53% (10/95) in engorged ticks, 9.09% (2/22) in eggs and 8% (4/50) in larvae pools, respectively. Phylogenetic analysis confirmed that sequences from eggs and larvae had closer relationship with those isolates from maternal engorged ticks with more than 99.7% homology and JMTV manifested with evolutional conservatism. Our study has identified for the first time that JMTV could be transmitted from mother generation to offspring of Haemaphysalis Longicornis. Nonetheless, the efficiency of transovarial transmission in JMTV and the significance of ticks as amplification hosts still need to be further illustrated.

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Recovery of complete genome sequences of Crimean-Congo haemorrhagic fever virus (CCHFV) directly from clinical samples: A comparative study between targeted enrichment and metagenomic approaches

D’Addiego,

Wand,

Afrough

et al. 2024

Journal of Virological Methods

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Application of Viral Metagenomics for Study of Emerging and Reemerging Tick-Borne Viruses

Cited by 16 publications

References 45 publications

Diversity of Viruses in Hard Ticks (Ixodidae) from Select Areas of a Wildlife-livestock Interface Ecosystem at Mikumi National Park, Tanzania

Diversity of Viruses in Hard Ticks (Ixodidae) from Select Areas of a Wildlife-livestock Interface Ecosystem at Mikumi National Park, Tanzania

Molecular evidence for potential transovarial transmission of Jingmen tick virus in Haemaphysalis longicornis fed on cattle from Yunnan Province, China

Recovery of complete genome sequences of Crimean-Congo haemorrhagic fever virus (CCHFV) directly from clinical samples: A comparative study between targeted enrichment and metagenomic approaches

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