Aiming at environmental control, we report the use of synchrotron radiation total reflection x-ray fluorescence (SRTXRF) analysis as a technique for trace elements determination in plants. The analyses were performed in two species (total plant) of Amaranthaceae family: Alternanthera brasiliana from four sites and Pfaffia glabrata from a single site. Some elements, such as P, S, K, Ca, Mn, Fe, Cu, Zn, Sr, and Pb were detected in all samples, and the elements Cl, Ti, Cr, Co, Ni, Br, Rb, Sr, Cd, Sn, Sb, and Ba were detected in some samples. The limit of detection (LOD) varied from 0.315 (Cu) to 121.4 (P) mg g 21 .