Application of Zeolitic Imidazolate Framework‐8 Nanoparticles for the Fluorescence‐Enhanced Detection of Nucleic Acids

Abstract

Stranded on a nanoparticle: For the first time, zeolitic imidazolate framework‐8 nanoparticles (ZIFNPs) have been used in an effective fluorescent sensing platform for nucleic acids. The DNA detection is accomplished in two steps: 1) ZIFNPs adsorb and quench the dye‐labeled single‐stranded DNA probe, and 2) subsequent hybridization of the probe with its target produces double‐stranded DNA, which detaches from the ZIFNP. The present system shows a high selectivity down to single‐base mismatch (see figure).

Fabrication of riboflavin electrochemical sensor based on homoadenine single-stranded DNA/molybdenum disulfide–graphene nanocomposite modified gold electrode

“…Previous studies on the interactions of DNA with Au have demonstrated that homoadenine singlestranded DNA (ssDNA) oligonucleotides can preferentially adsorb Au surface with high affinity predominantly via both the N6 exocyclic amino and the N7 atom of deoxyadenosine [22][23][24], implying that it can serve as an effective binding agent. In addition, it has been recently reported that ssDNA, especially those homoadenine oligonucleotides, can strongly bind to graphene by p-p stacking, hydrophobic interaction, hydrogen bonding, and van der Waals forces [24][25][26][27]. This suggests that the synthesis of MoS 2 Àgraphene nanocomposites facilitates the attachment of layered MoS 2 nanomaterials to the Au electrode.…”

Nanoarchitectonics of Biofunctionalized Metal–Organic Frameworks with Biological Macromolecules and Living Cells

“…For example, fluorescence‐assisted detection of nucleic acid (e.g., oligonucleotide) sequence associated with human immunodeficiency virus using ZIF‐8 as a carrier of FAM dye‐labeled ssDNA has been reported. This detection technique relied on the fluorescence emission signal of FAM‐labeled probe …”

Modulated light-activated electrochemistry at silicon functionalized with metal-organic frameworks towards addressable DNA chips

“…MOF has been shown to be a great platform for the adsorption of DNA (Liu et al 2012;Tian et al 2015;Zhang et al 2014;Zhao et al 2016). UIO-66-NH 2 was chosen to decorate silicon as it is highly stable in water and biocompatible in biological system compared to other kinds of MOF materials (Ruyra et al 2015).…”