Application RAPD Technique for Recognition Genotypes Tolerant to Drought in some of Bread Wheat

Gorji, A. H.; Darvish, F; Esmaeilzadehmoghadam, M.; Azizi, Farhad

doi:10.3923/ajbkr.2010.159.168

Cited by 5 publications

(1 citation statement)

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“…In additional to the specific or unique band derived from RAPD marker can be further developed as SCAR (sequence characterized amplified region) marker for rapid and simple identification of wheat cultivars. Such potentiality has also been highlighted by Belaj et al [73], Rajora and Rahman [74] and Gorji et al [75].…”

Section: Wheat Genotypes Fingerprintingmentioning

confidence: 61%

RAPD Fingerprinting and Genetic Relationships of Some Wheat Genotypes

Eid¹

2019

IJGG

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The genetic variability and relationships among 5 Egyptian wheat genotypes representing Sakha8, Sakha69, Sakha93, Sids1 and Gemmiza7 were analyzed using 8 random amplified polymorphic DNA (RAPD). A total of 77 loci (73 % polymorphic) in all 5 wheat genotypes was amplified and discriminated all the wheat genotypes. PIC, RP, MI, DP values were evaluated and revealed degree of genetic divergence among the cultivars used. A cluster based on UPGMA (Un-weighted Pair-Group Method with Arithmetic Mean) analysis was used to determine genetic similarities. The five wheat genotypes were divided into two main clusters. Cluster 1 was divided into two groups. In subgroup 1 were included genotype 1 and genotype 2. They seemed very close which might depict sharing of the genetic background among the genotypes. In consequence, the close genetic relationships are entirely alarming and may hinder further plant improvement. Genotype 5 was in subgroup 2. The second cluster was included genotype 3 and genotype 4. The same genotypes were also assessed in field conditions for structural analyses, which were carried out based on six yield components. The dendrogram created was comparatively analyzed with the RAPD dendrogram. This study additionally indicates that RAPD markers are useful for distinguishing and characterizing wheat cultivars. The genetic relatedness among these genotypes could provide useful information for conservation and selection of cross parents in breeding.

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Section: Wheat Genotypes Fingerprintingmentioning

confidence: 61%