Application rate trials with a nuclear polyhedrosis virus to control Spodoptera littoralis (Boisd.) on cotton in Egypt

Jones, Keith A.; Irving, N. S.; Grzywacz, D.; Moawad, G. M.; Hussein, Ahmed E.; Fargahly, A.

doi:10.1016/0261-2194(94)90047-7

Cited by 25 publications

(9 citation statements)

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“…Also, they showed high virulent effects on lepidopteran insects (El-Sheikh et al, 2011; El-Sheikh, 2014). Efficacy of SpliNPV was studied on S. littoralis under field (Topper et al, 1984;Jones et al, 1994) and laboratory (Grzywacz et al, 1998;Ashour et al, 2007) conditions as well as other spodopteran species (El-Sheikh, 2015b). This virus showed successful control of S. littoralis in the field as reported by Topper et al (1984) and showed high effectiveness against other species of S. frugiperda and S. exigua (El-Sheikh, 2015b) in a rate similar to what reported in the current study.…”

Section: Resultsmentioning

confidence: 99%

EFFICACY AND VIRULENCE OF Spodoptera littoralis NUCLEOPOLYHEDROVIRUS on S. littoralis LARVAL FEEDING AND SUSCEPTIBILITY

Abdel-Khalik

Lamiaa

El-Sheikh

et al. 2017

Zagazig Journal of Agricultural Research

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Entomopathogenic baculoviruses have long been recognized as vital environmentally safe alternatives to chemical pesticides. A study for investigating the virulence and susceptibility of Spodoptera littoralis larvae to S. littoralis nucleopolyhedrovirus (SpliNPV) isolate was carried out. Bioassay experiments on the 1 st and 2 nd larval instars using droplet feeding method revealed that polyhedral for 50% or 90% mortality of the 2 nd larval instar of S. littoralis was significantly lower compared with the 1 st instar. While, the 1 st instar was killed faster than the 2 nd one especially with the higher concentration. The susceptibility of the infected 2 nd instar when feeding on different hosts showed that larvae were more susceptible when feeding on eggplant leaves followed by lettuce leaves, artificial diet and tomato leaves as shown by larval weight and reduction in feeding. The reduction in both larval weight and feeding were significantly higher with 1x10 7 PIB/ml than 1x10 4 PIB/ml in the majority of cases. S. littoralis larvae showed more than 2 times of tolerant to the 3 rd passage of SpliNPV on the level of LC 50 and more than 18 times tolerant on the level of LC 90. The tolerant levels of S. littoralis larvae for the 3 rd passage of virus was associated with more than 20% increase in time taken for kill with no changes in DNA patterns regarding subsequent passages in S. littoralis larvae using a restriction enzyme EcoRI.

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Section: Resultsmentioning

confidence: 99%

EFFICACY AND VIRULENCE OF Spodoptera littoralis NUCLEOPOLYHEDROVIRUS on S. littoralis LARVAL FEEDING AND SUSCEPTIBILITY

Abdel-Khalik

Lamiaa

El-Sheikh

et al. 2017

Zagazig Journal of Agricultural Research

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“…Therefore, resistant strains of insect pests appeared in the field (Sawicki, 1986). An alternative control method that used in control strategy is biological agents such as natural enemies, nuclear polyhedrosis virus (Elnagar and El-Sheikh, 1990 and Jones et al, 1994 and Atia et al, 2016) and Bacillus thuringiensis and its derivative (Navon et al, 1983 and Moussa et al, 2016). However, S. littoralis and S. oryzae developed resistant against biological control agents (Salama et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Isolation of the Astacin-like metalloprotease coding gene (astl) and assessment of its insecticidal activity againstSpodoptera littoralisandSitophilus oryzae

Diab

Hussein

Ahmed

et al. 2019

Preprint

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14Astacin-like metalloprotease (astl) is a multi-domain metallopeptidase that has 15 protease activity against a number of organisms; including fish, frogs, birds and 16 insects. In this present investigation, the full length of astl cDNA was cloned 17 from spider species, Hasarius adansoni. Sequencing of the cloned astl cDNA 18 proved that its full length including 802 bp with 714bp open reading frame 19 encoding for 238 amino acids. The catalytic domain comprised of 489 nts was20 cloned and expressed by the yeast expression system Pichia pastoris and its 21 insecticidal activity was determined against two species of agricultural insects 22 Spodoptera littoralis (Lepidoptera:Noctuidae) and Sitophilus oryzae 23 (Coleoptera:Curculionidae). Bioassay was performed using three 2 24 concentrations (100,500 and 1000 ppm) for four days for S. littoralis and 14 25 days for S. oryzae. In addition, the astl was fused to the GNA snowdrop lectin 26 in the same frame and expressed in P. pastoris. The synergistic effect of astl 27 and GNA was examined on the S. littoralis larvae and S. oryzae adults. The 28 mortality percentages of the fused protein (Ha-astl/GNA) "1000 ppm " after 4 29 days, were 78.6%± 4.16 and 71.66% ±3.51 for first and second spodpotera 30 larval instars, respectively. While, lower mortality of the fused protein of the 31 same concentration was observed on S. oryzae adults, 49.3±2.08 %. 32 4 69pointed out that the neurotoxin protein from the Australian funnel web spider 70 Hadronyche versuta, Ѡ-hexatoxin-Hv1a was lethal to many insect species 71 when injected, while it is non-toxic through feeding. However, the Ѡ-72 hexatoxin-Hv1a/GNA fusion protein showed oral insecticidal activity against 73 insects from different orders. 74The agricultural and horticultural pest insects cause 40% loss of the crop yield 75 worldwide (Oerke et al., 1994) that is estimated by 17.7 billions dollars 76 annually (Oliveira et al., 2014). Spodoptera littoralisis one of the most 77 destructive agricultural lepidopteron insect pests that can damage many 78 economical crops such as cotton, maize, tomato and vegetables (Salama et al., 79 1970). The larvae of S. littoralis feed on the leaves, fruits, flowers, buds and 80 bolls of cotton making them useless (Bishari, 1934). Sitophilus oryzae is one of 81 the important insect pests of stored grains such as rice, wheat and their products 82 (Baloch, 1992). S. oryzae causes loss of weight, reduction in the nutritional 83 value and increasing the infection with other mites and fungi, therefore the 84 commercial value of the stored grains decreased (Madrid et al., 1990). These 85 agriculture pests are controlled by different methods such as chemical 86 insecticides which are extensively used in Egypt (Issa et al., 1984a; Issa et al., 87 1984b; and Abo-El-Ghar et al. , 1986). Therefore, resistant strains of insect 88 pests appeared in the field (Sawicki, 1986). An alternative control method that 89 used in control strategy is biological agents such as natural enemies, nuclear 90 polyhedrosi...

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“…The Egyptian cotton worm Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) is an important pest distributed throughout Africa, the Middle East and the Mediterranean region that causes extensive losses in many vegetable, fodder and fibre crops 1. In Turkey, it is a serious pest of cotton and many plants cultivated in greenhouses 2.…”

Section: Introductionmentioning

confidence: 99%

“…Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) is a member of Baculoviridae. It has been demonstrated that SpliNPV can be used in the field to control S. littoralis on cotton successfully 1. Several different SpliNPV isolates have been isolated from S. littoralis to date.…”

Section: Introductionmentioning

confidence: 99%

Comparative biological activities of a plaque‐purified variant and a Turkish native isolate of SpliNPV‐B against Spodoptera littoralis (Lepidoptera: Noctuidae)

Toprak

Bayram

Gurkan

2005

Pest Management Science

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The noctuid moth Spodoptera littoralis (Boisduval) is an important pest of many cultivated plants worldwide and five different geographical Nucleopolyhedrovirus (NPV) strains of this pest have been isolated to date. Two of these, a plaque-purified variant of the S. littoralis NPV from Morocco (SpliNPV-M2) and a SpliNPV isolated from field-infected S. littoralis larvae found in Turkey (SpliNPV-TR1), were compared biologically in terms of infectiveness (median lethal dose, LD50) for third instars and in terms of virulence (median lethal time, LT50) for neonates and third-instar S. littoralis larvae. The LD50 values of SpliNPV-TR1 and SpliNPV-M2 were 20.73 and 185.21 occlusion bodies (OBs)/larva, respectively, with non-overlapping confidence limits indicating they were significantly different. Thus, SpliNPV-M2 was found to be significantly less infective (about nine times higher LD50) than SpliNPV-TR1. The LT50 values of neonates for SpliNPV-M2 and SpliNPV-TR1 were 37 and 43.9 h at a concentration of 10(6) OBs ml(-1), respectively. For these same isolates, the LT50 values at a concentration of 3 x 10(6) OBs ml(-1) were calculated as 35.6 and 41.7 h, respectively. The LT(50) values of third instars for SpliNPV-M2 and SpliNPV-TR1 were 147.4 and 160.5 h, respectively, at a dose of 3000 OBs/larva and 145.4 and 152.4 h, respectively, for the same isolates at a dose of 20,000 OBs/larva. On the other hand, Helicoverpa armigera (Hübner) and Agrotis ipsilon (Hufnagel) revealed a lack of lethality of the SpliNPV-TR1 isolate.

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Application rate trials with a nuclear polyhedrosis virus to control Spodoptera littoralis (Boisd.) on cotton in Egypt

Cited by 25 publications

References 5 publications

EFFICACY AND VIRULENCE OF Spodoptera littoralis NUCLEOPOLYHEDROVIRUS on S. littoralis LARVAL FEEDING AND SUSCEPTIBILITY

EFFICACY AND VIRULENCE OF Spodoptera littoralis NUCLEOPOLYHEDROVIRUS on S. littoralis LARVAL FEEDING AND SUSCEPTIBILITY

Isolation of the Astacin-like metalloprotease coding gene (astl) and assessment of its insecticidal activity againstSpodoptera littoralisandSitophilus oryzae

Comparative biological activities of a plaque‐purified variant and a Turkish native isolate of SpliNPV‐B against Spodoptera littoralis (Lepidoptera: Noctuidae)

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