Applications of a DNA-electrochemical biosensor

Diculescu, Victor C.; Chiorcea-Paquim, Ana-Maria; Oliveira-Brett, Ana Maria

doi:10.1016/j.trac.2016.01.019

Cited by 173 publications

(98 citation statements)

References 66 publications

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“…A DNA-electrochemical biosensor is formed by an electrode (the electrochemical transducer) with a DNA probe immobilized on its surface (the biological recognition element) and is used to detect DNA-binding molecules (the analyte) that interact and induce changes in the DNA structure and electrochemical properties, which are further translated into an electrical signal [25][26][27][29][30][31]46,47]. Up to now, the G4-based electrochemical biosensors reported in the literature always used redox labels as amplification strategies.…”

Section: G4 Electrochemical Biosensorsmentioning

confidence: 99%

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Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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Section: G4 Electrochemical Biosensorsmentioning

confidence: 99%

“…The electrochemical research on DNA is of great relevance to explain many biological mechanisms, and the nucleic acids redox behavior and adsorption processes have been studied for a long time [24][25][26][27][28][29][30][31], but only recently started to be used for the detection of G4 configurations [19,31,32].…”

Section: Introductionmentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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“…Electrochemical technique has been considered as the best candidate for the on-site detection of molecules such DNA due to the high sensitivity, simplicity, reproducibility, low cost, relatively short analysis time and direct analysis, without any extraction, clean-up or preconcentration steps, and easy to miniaturization [6]. The ease and speed of preparation and of obtaining a new reproducible surface, the low residual current, porous surface and low cost of carbon paste are advantages of carbon paste electrode (CPE) over all other solid electrodes [7]. Furthermore, carbon is compatible with biological tissues more than other materials and as a result has broad application in electrochemical DNA sensors [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…The ease and speed of preparation and of obtaining a new reproducible surface, the low residual current, porous surface and low cost of carbon paste are advantages of carbon paste electrode (CPE) over all other solid electrodes [7]. Furthermore, carbon is compatible with biological tissues more than other materials and as a result has broad application in electrochemical DNA sensors [7][8][9][10][11][12]. Thus, carbon paste, in both unmodified and modified forms, is a material that has been widely utilized for electrochemical detection of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Application of promising carbonaceous materials in electrochemical DNA sensing

Karastogianni¹,

Deliyanni²,

Girousi³

2017

J Appl Bioanal

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The data reported in literature demonstrate that carbon paste electrodes (CPEs) are very suitable for a variety of applications and many works have thus been devoted in the development of new sensitive and selective electrode surfaces based on carbon paste as the electrode material of choice. The application of novel and promising carbonaceous materials, as electrode surfaces, is an issue of great concern. In this work the experimental results of the characterization and comparison of electrode surfaces based on alternatively prepared carbonaceous materials (activated carbon (B), HNO 3 oxidized activated carbon (B5), Ag impregnated activated carbon (B-Ag) and graphite oxide (GO), are being demonstrated. Scaning Electron Microscopy SEM), surface acidity, FTIR spectroscopy, XRD diffractometry and electrochemical techniques (cyclic voltammetry, differential pulse voltammetry) were applied in the characterization of novel carbonaceous materials aimed at electrochemical DNA sensing.

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“…1,2 Electrochemical DNA biosensor, [3][4][5] a device in the position of sequence-specific DNA analysis, has been vigorously pursued because of its outstanding advantages including specificity, sensitivity, portability and low-cost compared with the conventional methods, like the polymerase chain reaction (PCR), 6 surface plasmon resonance spectroscopy 7 and some others.…”

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confidence: 99%

An Electrochemical DNA Sensing Platform Using Carboxyl Functionalized Graphene as the Electrode Modified Material

Cheng

Yang

et al. 2017

J. Electrochem. Soc.

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With the expected characteristics of both promoting electric transfer rate and immobilizing -NH 2 labeled probe for the construction of electrochemical sensing platform for DNA determination, the carboxyl functionalized graphene (CFGR-COOH) was carried out from the carboxylation of graphene oxide (GO), partly reduction, addition of cyan groups (-CN). The X-ray photoelectron spectroscopy (XPS) results indicated the successfully prepared expected materials. Three kinds of novel electrochemical DNA biosensors were developed at CFGR-COOH modified glassy carbon electrode (CFGR-COOH/GCE), targeting at identifying specific DNA, the CFGR-COOH and PANI modified platinum electrode (CFGR-COOH/PANI/Pt), aiming at the further increase for the electrochemical performance of the prior biosensor with the synergistic effect between those two materials, and the hairpin probe/ horseradish peroxidase (HRP) labeled CFGR-COOH/GCE, which was constructed to prove the promise utilization of CFGR-COOH in various sensing platforms. Through the differential pulse voltammetry (DPV) detection, the linear ranges of 1.0 × 10 −6 to 1.0 × 10 −13 mol · L −1 , 1.0 × 10 −6 to 1.0 × 10 −14 mol · L −1 and 1.0 × 10 −6 to 1.0 × 10 −10 mol · L −1 were obtained for the above biosensors, coupling with the detection limits of 4.0 × 10 −14 mol · L −1 , 5.8 × 10 −15 mol · L −1 and 7.0 × 10 −11 mol · L −1 , respectively. The as-prepared CFGR-COOH showed potential application prospect as a valuable modification material in DNA sensing platforms.With the increase of understanding the key role of nucleic acids in providing and transferring enormous genetic information in organisms, considerable efforts have been devoted to the development of sequence-specific DNA detection. 1,2 Electrochemical DNA biosensor, 3-5 a device in the position of sequence-specific DNA analysis, has been vigorously pursued because of its outstanding advantages including specificity, sensitivity, portability and low-cost compared with the conventional methods, like the polymerase chain reaction (PCR), 6 surface plasmon resonance spectroscopy 7 and some others.Generally speaking, a biorecognition system and a transverter are covered in an electrochemical DNA biosensor. The formation of a biorecognition interface is achieved by the immobilization of a specific oligonucleotide sequence on the electrode surface, while the transverter plays a role in the conversion of the hybridization events into the electrochemical signals which is realized with the contributions of electrochemical indicators or the various responses from the single stranded probe to the helix structured oligonucleotides. 8 Glassy carbon electrode, 9 platinum electrode, 10 gold electrode, 11 as well as the carbon paste electrode 12 are often utilized as the substrates for DNA electrochemical detection. Previous reports 13 have proved that the DNA hybridization events cannot completely accomplished on the bare electrode because of the weak interactions and the existed overpotentials. With the rapid development and wide utiliz...

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Applications of a DNA-electrochemical biosensor

Cited by 173 publications

References 66 publications

Guanine Quadruplex Electrochemical Aptasensors

Guanine Quadruplex Electrochemical Aptasensors

Application of promising carbonaceous materials in electrochemical DNA sensing

An Electrochemical DNA Sensing Platform Using Carboxyl Functionalized Graphene as the Electrode Modified Material

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