Applications of aptamers in cancer cell biology

Phillips, Joseph A.; López-Colón, Dalia; Zhu, Zhi; Xu, Ye; Tan, Weihong

doi:10.1016/j.aca.2008.05.031

Cited by 109 publications

(71 citation statements)

References 51 publications

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“…Cancer cells are known to have specific proteins in their membranes and certain proteins are overexpressed in cancer cells. Proteins such as nucleolin, prostate-specific membrane antigen (PSMA) and others (see Phillips et al 2008;Orava et al 2010 for detailed review) can serve as cancer markers. Several DNA and RNA aptamers which selectively bind to cancer markers have been developed, although not all of them contain G-quadruplexes.…”

Section: For Detailed Review)mentioning

confidence: 99%

Potential uses of G-quadruplex-forming aptamers

Víglaský

Hianik

2013

gpb

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Abstract. Guanine quadruplex (G-quadruplex) structures are one of a number of structures which are capable of adopting aptamers. G-rich DNA or RNA has an increased propensity to form quadruplex structures which have unusual biophysical and biological properties. G-rich aptamers which form G-quadruplexes have several advantages over unstructured sequences: G-quadruplexes are non-immunogenic, thermodynamically and chemically stable and they have both higher resistance to various serum nucleases and an enhanced cellular uptake. These advantages have led to a number of synthetic oligonucleotides being studied for their potential use as therapeutic agents for cancer therapy and in the treatment of various other diseases. In addition to their suitability in the fields of medicine and biotechnology, these, highly specified, aptameric G-quadruplexes also have great potential in the further development of nano-devices; e.g. basic components in microarrays, microfluidics, sandwich assays and electrochemical biosensors. This review summarizes the biophysical properties of G-quadruplexes and highlights the importance of the stability and recognition properties of aptamers. Examples of the application of aptamers in medical therapy and in biosensors are also discussed.

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Section: For Detailed Review)mentioning

confidence: 99%

Potential uses of G-quadruplex-forming aptamers

Víglaský

Hianik

2013

gpb

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“…Счита се, че съчетана с традиционно приетия за скрининг FOBT тест, трансфериновата тест-лента би подобрила ефикасността при скрининг не само на колоректален карцином, но и на други видове рак (езофагеналния или рак на стомаха). Освен това, вече са селектирани специфични аптамери (ДНК и РНК малки синтетични олигонуклеотиди) за CRC клетъчни линии, прилагайки cell-SELEX (клетъчно базиран систематичен подбор на лиганди чрез експоненциално обогатяване) и се очаква те да се въведат в практиката като прогностични маркери и ранни детектори за CRC и други ракови заболявания (Ellington and Szostak, 1990;Phillips et al, 2008;Sefah et al, 2010;Tuerk and Gold, 1990). Freire, 1995;Privalov and Potekhin, 1986;Sturtevant, 1987):…”

Section: литературен обзорunclassified

Thermodynamic profile of the plasma proteome for malignancies

Тодикова¹

2013

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“…Therefore, it is highly probable that following cell-SELEX, several aptamers directed against different cell surface targets are produced. This can be greatly beneficial when targeting a disorder, such as cancer, that has a heterogeneous composition [18,46,47]. Table 1 …”

Section: Cell Selexmentioning

confidence: 99%

Aptamer Therapeutics: The 21st Century`s Magic Bullet of Nanomedicine

Shigdar¹,

Luczo²,

Wei³

et al. 2010

TOPROCJ

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Aptamers, also known as chemical antibodies, are short single-stranded DNA, RNA or peptide molecules. These molecules can fold into complex three-dimensional structures and bind to target molecules with high affinity and specificity. The nucleic acid aptamers are selected from combinatorial libraries by an iterative in vitro selection procedure known as systematic evolution of ligands by exponential enrichment (SELEX). As a new class of therapeutics and drug targeting entities, bivalent and multivalent aptamer-based molecules are emerging as highly attractive alternatives to monoclonal antibodies as targeted therapeutics.Aptamers have several advantages, offering the possibility of overcoming limitations of antibodies: 1) they can be selected against toxic or non-immunogenic targets; 2) aptamers can be chemically modified by using modified nucleotides to enhance their stability in biological fluids or via incorporating reporter molecules, radioisotopes and functional groups for their detection and immobilization; 3) they have very low immunogenicity; 4) they display high stability at room temperature, in extreme pH, or solvent; 5) once selected, they can be chemically synthesized free from cell-culturederived contaminants, and they can be manufactured at any time, in large amounts, at relatively low cost and reproducibly; 6) they are smaller and thus can diffuse more rapidly into tissues and organs, leading to faster targeting in drug delivery; 7) they have lower molecular weight that can lead to faster body clearance, resulting in a low background noise for imaging and minimizing the radiation dose to the patient in diagnostic imaging. Thus, the high selectivity and sensitivity, ease of screening and production, chemical versatility as well as stability make aptamers a class of highly attractive agents for the development of novel therapeutics, targeted drug delivery vehicles and molecular imaging.In the review, we will discuss the latest technological advances in developing aptamers, its application as a novel class of drug on its own, as well as in surface functionalization of both polymer nanoparticles or nanoliposomes in the treatment of cancer, viral and autoimmune diseases.

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Applications of aptamers in cancer cell biology

Cited by 109 publications

References 51 publications

Potential uses of G-quadruplex-forming aptamers

Potential uses of G-quadruplex-forming aptamers

Thermodynamic profile of the plasma proteome for malignancies

Aptamer Therapeutics: The 21st Century`s Magic Bullet of Nanomedicine

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