Applications of capillary electrophoresis with electrochemical detection in pharmaceutical and biomedical analyses

Wang, Anbao; Fang, Yuzhi

doi:10.1002/(sici)1522-2683(20000401)21:7<1281::aid-elps1281>3.0.co;2-7

Cited by 56 publications

(37 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This impact has been expressed in an enormous amount of CE application articles and many review articles [1][2][3][4][5]. Only in one application, DNA sequencing analysis, the explosive growth is evident in the last two years [6][7][8].…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

“…In this case, the electroosmotic flow is suppressed and the ionic species are separated by seiving effect. CE has been coupled to different detection methods including ultraviolet UV-visible absorption [11], conductimetry, mass spectrometry (MS), patch clamp, electrochemical (EC) [4,13] or LIFD [14,15]. This versatility of separation and detection methods has allowed analyzing a wide spectrum of substances ranging from ions to proteins.…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

“…Besides, the resulting CE waste is safe for the environment, while HPLC produces large amounts of organic solvent waste, and GC volatile compounds, that are sometimes toxic which pollute the environment [3]. All these advantages convert CE as almost ideal for the analysis of numerous endogenous and exogenous substances present in biological fluids [4,16,17].…”

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

“…EC detection has better mass sensitivity; it does not need sample derivatization, and is tunable because its selectivity is a function of the potential applied [22]. But it still has poor concentration sensitivity and difficult manipulation that makes the technique the domain of the specialist in electrochemistry [4].…”

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

“…In the last few years, CE has been extensively used as a separation technique in the pharmaceutical industry [4,17,49,57,58], and in the clinical chemistry modern laboratory [59][60][61][62][63]. This success is due to, its quick analysis time, low costs, small sample volume convenient for microdialysates and capillary blood samples, the versatility to resolve different molecules and high resolution power [16].…”

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

See 4 more Smart Citations

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

show abstract

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Section: Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

Section: Pharmaceutical Clinical and Forensic Applicationsmentioning

confidence: 99%

See 3 more Smart Citations

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

show abstract

Recent advances in capillary electrophoresis of peptides

Kašička

2001

Electrophoresis

100

View full text Add to dashboard Cite

The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, including zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography, to analysis, preparation and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, and covers the methodological aspects of capillary electroseparations of peptides, such as strategy and rules for the rational selection of separation mode and experimental conditions, sample treatment, suppression of peptide adsorption to the inner capillary wall, new developments in individual separation modes and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis for determination of purity, determination in biomatrices, monitoring of physical and chemical changes and enzymatic conversions, amino acid and sequence analysis and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

show abstract

Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)

et al. 2001

View full text Add to dashboard Cite

In this paper, capillary electrophoresis in clinical and forensic analysis is reviewed on the basis of the literature of 1999, 2000 and the first papers in 2001. An overview of progress relevant examples for each major field of application, namely (i) analysis of drug seizures, explosives residues, gunshot residues and inks, (ii) monitoring of drugs, endogenous small molecules and ions in biofluids and tissues, (iii) general screening for serum proteins and analysis of specific proteins (carbohydrate deficient transferrin, alpha1-antitrypsin, lipoproteins and hemoglobins) in biological fluids, and (iv) analysis of nucleic acids and oligonucleotides in biological samples, including oligonucleotide therapeutics, are presented.

show abstract

Applications of capillary electrophoresis with electrochemical detection in pharmaceutical and biomedical analyses

Cited by 56 publications

References 103 publications

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Recent advances in capillary electrophoresis of peptides

Advances of capillary electrophoresis in clinical and forensic analysis (1999-2000)

Contact Info

Product

Resources

About