Applications of CRISPR/Cas Beyond Simple Traits in Crops

Khan, Sultan Habibullah; Ghouri, Muhammad Zubair; Aslam, Sabin; Mubarik, Muhammad Salman; Khan, Zulqurnain; Ahmad, Muhammad Qadir; Sajjad, Muhammad

doi:10.1007/978-981-15-7142-8_8

Cited by 1 publication

(9 citation statements)

References 181 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the past 7 years, this technique has been widely used in almost every aspect of life, including agriculture, medicine, and the environment. After successfully using gene editing to change basic traits, scientists are now working on more ambitious genomics projects to solve the challenges of food security in the wake of the growing population and climate change risks ( Ahmad et al., 2021 ; Aslam et al., 2021 ; Khan et al., 2021 ; Ashraf et al., 2022 ; Wang Z et al., 2022 ).…”

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

“…In the second phase of the CRISPR/Cas9 execution mechanism, the lengthy Pre- crRNA is purposely transcribed from the CRISPR nucleus and replicated into crRNAs using Cas9 proteins. Recently, it was found that tracrRNA is also processed during Streptococcus pyogenes pre-crRNA synthesis ( Aslam et al., 2021 ; Khan et al., 2021 ; Ashraf et al., 2022 ). The tracrRNA is linked to the repeat and the 46-crRNA site through complementary base pairing, which facilitates the conversion of precrRNA into crRNA.…”

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

“…The CRISPR/Cas system has become a potent tool for precise genome editing with a wide range of uses, including genetic code rewriting, base editing, knockout, and knock-in. Both Cas9 and Cas12 are among the most frequently employed Cas nucleases for DNA manipulation and are members of the Class 2 CRISPR/Cas system ( Khan et al., 2021 ; Wang Z et al., 2022 ; Ahmad M., 2022 ; Ashraf et al., 2022 ). Although both CRISPRi and CRISPRa could be utilized to control transcription, Cas9 nuclease was unable to target RNA at the post-transcriptional level.…”

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

“…An important mutation site can have the fewest/no off-target consequences by selecting the right target site(s). In model organisms, such as rice crops, a wide range of bioinformatics online tools are available for the creation of sgRNA as well as the discovery of off-target ( Khan et al., 2021 ; Ashraf et al., 2022 ; Chen et al, 2022 ). Designing the oligonucleotide or primers that are relevant to the target site comes next after the target site has been found.…”

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

“…It may seem apparent, but when developing a CRISPR/Cas9 vector for an experiment, it is important to maximize on-target activity while limiting off-target activity ( Khan et al., 2021 ; ( Molla et al., 2021 ; Kumar et al., 2022 ; LaManna et al., 2022 ; Li et al., 2022 ; Liu et al., 2022a ; Liu et al., 2022b )…”

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

See 4 more Smart Citations

Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security

Ahmad

2023

Front. Plant Sci.

View full text Add to dashboard Cite

Genome editing techniques are being used to modify plant breeding, which might increase food production sustainably by 2050. A product made feasible by genome editing is becoming better known, because of looser regulation and widespread acceptance. The world’s population and food supply would never have increased proportionally under current farming practices. The development of plants and food production has been greatly impacted by global warming and climate change. Therefore, minimizing these effects is crucial for agricultural production that is sustainable. Crops are becoming more resilient to abiotic stress because of sophisticated agricultural practices and a better understanding of the abiotic stress response mechanism. Both conventional and molecular breeding techniques have been used to create viable crop types both processes are time-consuming. Recently, plant breeders have shown an interest in genome editing approaches for genetic manipulation that use clustered regularly interspaced short palindromic repeats (CRISPR/Cas9). To ensure the security of the food supply in the future, plant kinds with desired traits must be developed. A completely new era in plant breeding has begun because of the revolution in genome editing techniques based on the CRISPR/CRISPR-associated nuclease (Cas9) systems. All plants may effectively target a particular gene or group of loci using Cas9 and single-guide RNA (sgRNA). CRISPR/Cas9 can thereby save time and labor compared to conventional breeding methods. An easy, quick, and efficient method for directly altering the genetic sequences in cells is with the CRISPR and Cas9 systems. The CRISPR-Cas9 system, which was developed from components of the earliest known bacterial immune system, allows for targeted gene breakage and gene editing in a variety of cells/RNA sequences to guide endonuclease cleavage specificity in the CRISPR-Cas9 system. Editing can be directed to practically any genomic site by altering the guide RNA (gRNA) sequence and delivering it to a target cell along with the Cas9 endonuclease. We summarize recent CRISPR/Cas9 plant research findings, investigate potential applications in plant breeding, and make predictions about likely future breakthroughs and approaches to food security through 2050.

show abstract

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

Section: Why Crispr-cas Ge Better Than Zfns and Talens Genome Editing?mentioning

confidence: 99%

See 3 more Smart Citations

Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security

Ahmad

2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

Applications of CRISPR/Cas Beyond Simple Traits in Crops

Cited by 1 publication

References 181 publications

Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security

Plant breeding advancements with “CRISPR-Cas” genome editing technologies will assist future food security

Contact Info

Product

Resources

About