Applications of CRISPR/Cas System in Plants

Khan, Sultan Habibullah; Tariq, Hooran; Farooq, Iqra; Tasleeem, Hira; Ghouri, Muhammad Zubair; Mubarik, Muhammad Salman; Khan, Zulqurnain

doi:10.1007/978-981-16-6305-5_9

Cited by 3 publications

(3 citation statements)

References 134 publications

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“…CRISPR–Cas has emerged as a valuable tool in agriculture due to its unequaled ability to accurately change plant genomes ( Khan et al, 2022 ). It has changed present breeding processes and assisted in the development of novel varieties of crops with desirable traits ( Zhu, Li & Gao, 2020 ), thus making it possible to domesticate new species within a short period ( Wolter, Schindele & Puchta, 2019 ).…”

Section: Crop Improvement Using Crispr-cas As Genetic Engineering Toolmentioning

confidence: 99%

“…The production of herbicide-resistant crops using CRISPR-Cas has become an efficient technique for controlling weeds, and new technologies for transferring herbicide resistance to plants ( Han & Kim, 2019 ). This is achieved by targeting endogenous genes like acetolactate synthase (ALS), cellulose synthase A catalytic subunit 3 (CESA3), 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), and splicing factor 3B subunit 1 (SF3B1) ( Hussain et al, 2021 ; Khan et al, 2022 ). Herbicide tolerance can be achieved by the changes in the ALS gene of amino acid according to studies of naturally occurring point mutations in the ALS gene ( McNaughton et al., 2005 ).…”

Section: Impact Of Crispr-cas In Managing the Environmental Stress On...mentioning

confidence: 99%

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Adoption of CRISPR-Cas for crop production: present status and future prospects

Akanmu,

Asemoloye,

Marchisio

et al. 2024

PeerJ

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Background Global food systems in recent years have been impacted by some harsh environmental challenges and excessive anthropogenic activities. The increasing levels of both biotic and abiotic stressors have led to a decline in food production, safety, and quality. This has also contributed to a low crop production rate and difficulty in meeting the requirements of the ever-growing population. Several biotic stresses have developed above natural resistance in crops coupled with alarming contamination rates. In particular, the multiple antibiotic resistance in bacteria and some other plant pathogens has been a hot topic over recent years since the food system is often exposed to contamination at each of the farm-to-fork stages. Therefore, a system that prioritizes the safety, quality, and availability of foods is needed to meet the health and dietary preferences of everyone at every time. Methods This review collected scattered information on food systems and proposes methods for plant disease management. Multiple databases were searched for relevant specialized literature in the field. Particular attention was placed on the genetic methods with special interest in the potentials of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and Cas (CRISPR associated) proteins technology in food systems and security. Results The review reveals the approaches that have been developed to salvage the problem of food insecurity in an attempt to achieve sustainable agriculture. On crop plants, some systems tend towards either enhancing the systemic resistance or engineering resistant varieties against known pathogens. The CRISPR-Cas technology has become a popular tool for engineering desired genes in living organisms. This review discusses its impact and why it should be considered in the sustainable management, availability, and quality of food systems. Some important roles of CRISPR-Cas have been established concerning conventional and earlier genome editing methods for simultaneous modification of different agronomic traits in crops. Conclusion Despite the controversies over the safety of the CRISPR-Cas system, its importance has been evident in the engineering of disease- and drought-resistant crop varieties, the improvement of crop yield, and enhancement of food quality.

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Section: Crop Improvement Using Crispr-cas As Genetic Engineering Toolmentioning

confidence: 99%

Section: Impact Of Crispr-cas In Managing the Environmental Stress On...mentioning

confidence: 99%

Adoption of CRISPR-Cas for crop production: present status and future prospects

Akanmu,

Asemoloye,

Marchisio

et al. 2024

PeerJ

View full text Add to dashboard Cite

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“…CRISPR/Cas9 has been used to knock out hundreds of genes and increases tolerance to abiotic stresses. For the success of CRISPR/Cas9 applications, single-guided RNA (sgRNA) must be expressed in the host organism to edit multiple genes [ 103 , 104 ]. Multiple genes can be edited if all genes have the same sequence.…”

Section: Crispr/cas9-mediated Salt Tolerance In Soybeanmentioning

confidence: 99%

Molecular Tools and Their Applications in Developing Salt-Tolerant Soybean (Glycine max L.) Cultivars

et al. 2022

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Abiotic stresses are one of the significant threats to soybean (Glycine max L.) growth and yields worldwide. Soybean has a crucial role in the global food supply chain and food security and contributes the main protein share compared to other crops. Hence, there is a vast scientific saddle on soybean researchers to develop tolerant genotypes to meet the growing need of food for the huge population. A large portion of cultivated land is damaged by salinity stress, and the situation worsens yearly. In past years, many attempts have increased soybean resilience to salinity stress. Different molecular techniques such as quantitative trait loci mapping (QTL), genetic engineering, transcriptome, transcription factor analysis (TFs), CRISPR/Cas9, as well as other conventional methods are used for the breeding of salt-tolerant cultivars of soybean to safeguard its yield under changing environments. These powerful genetic tools ensure sustainable soybean yields, preserving genetic variability for future use. Only a few reports about a detailed overview of soybean salinity tolerance have been published. Therefore, this review focuses on a detailed overview of several molecular techniques for soybean salinity tolerance and draws a future research direction. Thus, the updated review will provide complete guidelines for researchers working on the genetic mechanism of salinity tolerance in soybean.

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