Applications of display technologies to proteomic analyses

Abstract

With the rapid accumulation of genetic information, development of general experimental approach suitable for large scale annotation and pro®ling of the whole proteome have become one of the major challenges in postgenomic era. Biomolecular display technologies, which allow expressing of a large pool of modularly coded biomolecules, are extremely useful for accessing and analyzing protein diversity and interaction pro®le on a large scale. Recent advances in protein display technologies and their applications to proteomic analyses have been discussed.

Display technologies: Application for the discovery of drug and gene delivery agents☆

“…Although linking polypeptides to their encoding DNA extends to other functional selection methods, such as the yeast two-hybrid system [47][48][49]187], assays for intracellular interaction are conventionally considered as a separate category. While display scaffolds presenting polypeptides in the library vary, the ability to link an individual polypeptide selected based on its particular properties to the coding nucleic acid (cDNA or mRNA) is the feature that makes it possible to identify, isolate and amplify the desired library members [188]. There are five major display technologies, which have evolved in parallel ( Fig.…”