Applications of DNA-nanozyme-based sensors

Yu, Renzhong; Wang, Rui; Wang, Zhaoyin; Zhu, Qinshu; Dai, Zhihui

doi:10.1039/d0an02368j

Cited by 41 publications

(23 citation statements)

References 141 publications

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“…The field of nanozyme endeavors to engineer nanomaterials as enzyme mimics, and most current nanozymes have the same substrates and products as enzymes. − Nanozymes have attracted the attention of many researchers for their excellent stability, low cost, and versatile surface chemistry. Nanozymes might replace enzymes for biomedical, − bioanalytical, − and environmental applications. , One type of nanozyme immobilizes small molecular catalysts on nanoparticles (NPs), where the nanoparticle core serves only as a support . A representative example was reported by Manea et al ., where they functionalized AuNPs with thiolated azacrown and loaded it with Zn 2+ ions to mimic RNase activity. , This type of nanozyme is considered as an immobilized catalyst and thus is expected to have catalytic turnovers.…”

Section: Introductionmentioning

confidence: 99%

Nanozyme Catalytic Turnover and Self-Limited Reactions

2021

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Enzymes have catalytic turnovers. The field of nanozyme endeavors to engineer nanomaterials as enzyme mimics. However, a discrepancy in the definition of "nanozyme concentration" has led to an unrealistic calculation of nanozyme catalytic turnovers. To date, most of the reported works have considered either the atomic concentration or nanoparticle (NP) concentration as nanozyme concentration. These assumptions can lead to a significant under-or overestimation of the catalytic activity of nanozymes. In this article, we review some classic nanozymes including Fe 3 O 4 , CeO 2 , and gold nanoparticles (AuNPs) with a focus on the reported catalytic activities. We argue that only the surface atoms should be considered as nanozyme active sites, and then the turnover numbers and rates were recalculated based on the surface atoms. According to the calculations, the catalytic turnover of peroxidase Fe 3 O 4 NPs is validated. AuNPs are self-limited when performing glucose-oxidase like activity, but they are also true catalysts. For CeO 2 NPs, a self-limited behavior is observed for both oxidase-and phosphatase-like activities due to the adsorption of reaction products. Moreover, the catalytic activity of single-atom nanozymes is discussed. Finally, a few suggestions for future research are proposed.

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Section: Introductionmentioning

confidence: 99%

Nanozyme Catalytic Turnover and Self-Limited Reactions

2021

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“…A broader approach to improve catalytic activity in nanozymes has been recently reported, based on the use of hybrid systems combining biomolecules-nanoparticles or metalorganic systems. 104,105 This constitutes an interesting tactic to stimulate the bio-electro-catalyzed oxidation of glucose by the electrical contact of an integrated enzyme electrode that stimulates the bio-electro-catalyzed oxidation of glucose. A recent work by Katz et al 106 reported a related scheme through the use of an apoflavoenzyme with a relay-FAD-cofactor dyad, electrically contacting them to obtain a hybrid (named electroenzyme), while the engineering of the apo-glucose oxidase (apo-Gox) was achieved using a ferrocene-tethered FAD-cofactor.…”

Section: Journal Of Applied Physicsmentioning

confidence: 99%

Next generation of nanozymes: A perspective of the challenges to match biological performance

Goya

Mayoral

Winkler

et al. 2021

Journal of Applied Physics

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Nanomaterials with enzyme-like activity have been the spotlight of scientific and technological efforts to substitute natural enzymes, not only in biological research but also for industrial manufacturing, medicine, and environment healing. Notable advancements in this field along the last years relied on to the rational design of single-atom active sites, knowledge of the underlying atomic structure, and realistic ab initio theoretical models of the electronic configuration at the active site. Thus, it is plausible that a next generation of nanozymes still to come will show even improved catalytic efficiency and substrate specificity. However, the dynamic nature of the protein cage surrounding most active sites in biological enzymes adds a flexible functionality that possess a challenge for nanozyme's mimicking of their natural counterparts. We offer a perspective about where the main strategies to improve nanozymes are headed and identify some of the big challenges faced along the road to better performance. We also outline some of the most exciting bio-inspired ideas that could potentially change this field.

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“…Additionally, a high specific surface area of MN electrodes enhances their sensitivity. The functionalization of MN electrodes with nanomaterials and biomolecules, such as antibodies, aptamers, and enzymes, can improve their biorecognition efficiency and enable sensing of different types of analytes (Ozer et al, 2020; R. Yu et al, 2021). Moreover, the MN devices can be connected to portable wireless devices, including smartphones and microcontrollers, for data acquisition and analysis.…”

Section: Mns For Multibiosensingmentioning

confidence: 99%

Advances and challenges in developing smart, multifunctional microneedles for biomedical applications

Tavafoghi

Nasrollahi

Karamikamkar

et al. 2022

Biotech & Bioengineering

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Microneedles (MNs) have been developed as minimally invasive tools for diagnostic and therapeutic applications. However, in recent years, there has been an increasing interest in developing smart multifunctional MN devices to provide automated and closed-loop systems for body fluid extraction, biosensing, and drug delivery in a stimuli-responsive manner. Although this technology is still in its infancy and far from being translated into the clinic, preclinical trials have shown some promise for the broad applications of multifunctional MN devices. The main challenge facing the fabrication of smart MN patches is the integration of multiple modules, such as drug carriers, highly sensitive biosensors, and data analyzers in one miniaturized MN device. Researchers have shown the feasibility of creating smart MNs by integrating stimuli-responsive biomaterials and advanced microscale technologies, such as microsensors and microfluidic systems, to precisely control the transportation of biofluids and drugs throughout the system. These multifunctional MN devices can be envisioned in two distinct strategies. The first type includes individual drug delivery and biosensing MN units with a microfluidic system and a digital analyzer responsible for fluid transportation and communication between these two modules.The second type relies on smart biomaterials that can function as drug deliverers and biosensors by releasing drugs in a stimuli-responsive manner. These smart biomaterials can undergo structural changes when exposed to external stimuli, such as pH and ionic changes, mimicking the biological systems. Studies have demonstrated a high potential of hydrogel-based MN devices for a wide variety of biomedical applications, such as drug and cell delivery, as well as interstitial fluid extraction. Biodegradable hydrogels have also been advantageous for fabricating multifunctional MNs due to their high loading capacity and biocompatibility with the drug of choice. Here, we first review a set of MN devices that can be employed either for biosensing or delivery of multiple target molecules and compare them to the conventional and more simple systems, which are mainly designed for singlemolecule sensing or delivery. Subsequently, we expand our insight into advanced MN systems with multiple competencies, such as body fluid extraction, biosensing,

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Applications of DNA-nanozyme-based sensors

Abstract: In this review, the research progress of the sensors based on the DNA-nanozymes were summarized.

Cited by 41 publications

References 141 publications

Nanozyme Catalytic Turnover and Self-Limited Reactions

Nanozyme Catalytic Turnover and Self-Limited Reactions

Next generation of nanozymes: A perspective of the challenges to match biological performance

Advances and challenges in developing smart, multifunctional microneedles for biomedical applications

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