Applications of Engineered Skin Tissue for Cosmetic Component and Toxicology Detection

Abstract

The scale of the cosmetic market is increasing every day. There are many safety risks to cosmetics, but they benefit people at the same time. The skin can become red, swollen, itchy, chronically toxic, and senescent due to the misuse of cosmetics, triggering skin injuries, with contact dermatitis being the most common. Therefore, there is an urgent need for a system that can scientifically and rationally detect the composition and perform a toxicological assessment of cosmetic products. Traditional detection methods rely on instrumentation and method selection, which are less sensitive and more complex to perform. Engineered skin tissue has emerged with the advent of tissue engineering technology as an emerging bioengineering technology. The ideal engineered skin tissue is the basis for building good in vitro structures and physiological functions in this field. This review introduces the existing cosmetic testing and toxicological evaluation methods, the current development status, and the types and characteristics of engineered skin tissue. The application of engineered skin tissue in the field of cosmetic composition detection and toxicological evaluation, as well as the different types of tissue engineering scaffold materials and three-dimensional (3D) organoid preparation approaches, is highlighted in this review to provide methods and ideas for constructing the next engineered skin tissue for cosmetic raw material component analysis and toxicological evaluation.

Guidelines for Manufacturing and Application of Organoids: Skin

“…In Europe, animal testing for cosmetics has been banned since 2004, and a lot of investment has been made in the development of alternative animal testing methods ( 4 ). Based on this background, alternative testing methods using artificial skins for safety evaluation are the first to be developed ( 5 ) and put into practice. Currently, a human skin model created by 3D culture of human-derived skin cells has been commercialized and is being used in internationally certified tests to evaluate local toxicity such as skin corrosion/irritation (OECD TG 431, TG 439, TG 498, ISO 10993-23) ( 6 - 9 ).…”