Applications of Fluorescence Correlation Spectroscopy: Polydispersity Measurements

Starchev, Konstantin; Buffle, Jacques; Pérez, Elı́as

doi:10.1006/jcis.1999.6128

Cited by 45 publications

(56 citation statements)

References 20 publications

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“…The variance of the points of the next 30 replica FCS autocorrelation curves as well as the SN ratio was calculated according to Eq. [6].…”

Section: Methods and Experimentsmentioning

confidence: 95%

“…Therefore, the SN ratio is proportional to √ T (see Eqs. [3] and [6]) as in PCS measurements. Figure 1 shows the variance of the normalized autocorrelation function vs delay time for different accumulation times.…”

Section: Dependence Of the Noise And Sn Ratio On The Accumulation Timementioning

confidence: 99%

“…Finally, it has been shown recently (5,6,8) that Monte Carlo simulations are useful tools for the validation of theoretical and experimental FCS results. In some cases (5, 6) such a simulation has been performed by adding Gaussian noise to the calculated deterministic autocorrelation function.…”

Section: Introductionmentioning

confidence: 97%

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Noise on Fluorescence Correlation Spectroscopy

Starchev

Rička

Buffle

2001

Journal of Colloid and Interface Science

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“…The variance of the points of the next 30 replica FCS autocorrelation curves as well as the SN ratio was calculated according to Eq. [6].…”

Section: Methods and Experimentsmentioning

confidence: 95%

Section: Dependence Of the Noise And Sn Ratio On The Accumulation Timementioning

confidence: 99%

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Noise on Fluorescence Correlation Spectroscopy

Starchev

Rička

Buffle

2001

Journal of Colloid and Interface Science

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“…For noninteracting, freely diffusing, fluorescent particles with different translational diffusion coefficients and brightness, a closedform expression of Eq. (1) can be derived: [59][60][61][62][63] GðrÞ…”

Section: Fluorescence Correlation Spectroscopymentioning

confidence: 99%

Single‐walled tubulin ring polymers

et al. 2007

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An unusual class of nanoscopic, ring‐shaped, single‐walled biopolymers arises when αβ‐tubulin is mixed with certain small peptides obtained from various marine organisms and cyanobacteria. The single‐ring structures, whose mean molecular weight depends on the specific peptide added to the reaction mixture, usually have sharp mass distributions corresponding, e.g., to rings containing eight tubulin dimers (when the added peptide is cryptophycin) and 14 dimers (e.g., with dolastatin). Although the ring‐forming peptides have been shown to possess antimitotic properties when tested with cultured eukaryotic cells (and thus have generated considerable interest as possible agents to be used in the treatment of cancer), it is not our intention to extensively discuss the potential pharmacological properties of the peptides. Rather, we will review the polymeric structures that form and illustrate how certain physical techniques can be used to characterize their properties and interactions. The nanoscopic size and particular geometry of the individual rings make them appropriate targets for scattering and hydrodynamic techniques that provide details about their structure in solution, but it is necessary to relate measured data to postulated structures by nontrivial, albeit straight‐forward, mathematical, and computational means. We will discuss how this is done when one uses such methods as small angle neutron scattering, dynamic light scattering, fluorescence correlation spectroscopy, and sedimentation velocity measurements. Moreover, we show that, by using several techniques, one can eliminate degeneracy to provide better discrimination between model structures. © 2007 Wiley Periodicals, Inc. Biopolymers 86: 424–436, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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“…34 It will be also not further discussed how FCS can assist in analysing the photokinetics in conjugated polymers, 35 or detect molecular mobility at interfaces, 36,37 nor will this review cover FCS studies performed with nanoparticles. [38][39][40][41][42][43][44] With respect to the latter topic, I refer to the review on FCS studies in the journal "Current Opinion in Colloid & Interface Science" by Koynov et al 45 Fluorescence correlation spectroscopy…”

mentioning

confidence: 99%