Applications of Gas Chromatography for the Analysis of Tricyclic Antidepressants in Biological Matrices

Manousi, Natalia; Samanidou, Victoria

doi:10.3390/separations6020024

Cited by 8 publications

(9 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The literature reports several reviews focused on GC applications for bioanalysis for specific classes of drugs and their metabolites [65][66][67]. Some examples of analytical methods based on GC techniques are listed in Table 1.…”

Section: Gc Analysis Of Drugs and Metabolites In Main Biological Matr...mentioning

confidence: 99%

Recent Applications of Gas Chromatography in Bioanalysis

David¹,

Moldoveanu²

2023

Novel Aspects of Gas Chromatography and Chemometrics

View full text Add to dashboard Cite

Bioanalysis involves a broad range of chemical analyzes. These analyzes include that of biotics, such as natural components of living organisms, as well as xenobiotics, such as drugs and their metabolites in biological systems. Because many biotics and xenobiotics are not volatile molecules, the main technique for bioanalysis is high-performance liquid chromatography (HPLC) and the limitation of GC utilization is caused by the fact that GC is applicable only to volatile samples. However, gas chromatography (GC) in particular coupled with mass spectrometry (MS) as detection is also a very useful technique in bioanalysis. A considerable number of analytes in bioanalysis are volatile or can be made volatile following, for example, derivatization. As a result, GC (and GC/MS) are commonly utilized for the analysis of biotics, such as amino acids, fatty acids, various metabolites in biological fluids, and in particular of a large number of xenobiotics, such as drugs, drug metabolites, toxicants, and certain metabolic compounds caused by toxicants. The chapter will present progress in the GC methodology for extending its applicability to bioanalysis and will provide a review of more recent applications.

show abstract

Section: Gc Analysis Of Drugs and Metabolites In Main Biological Matr...mentioning

confidence: 99%

Recent Applications of Gas Chromatography in Bioanalysis

David¹,

Moldoveanu²

2023

Novel Aspects of Gas Chromatography and Chemometrics

View full text Add to dashboard Cite

show abstract

“…Tricyclic antidepressants (TCAs) are pharmacological agents that are commonly recommended for the treatment of major depressive disorders and anxiety disorders [13,14]. The discovery of these compounds occurred in the 1950s [15,16].…”

Section: Introductionmentioning

confidence: 99%

Development of Visible Spectrophotometric Methods for the Determination of Tricyclic Antidepressants Based on Formation of Molecular Complexes with p-Benzoquinones

Ciuca,

Racovita

2023

IJMS

View full text Add to dashboard Cite

Tricyclic antidepressants are commonly employed in the management of major depressive disorders. The present work describes two visible (VIS) spectrophotometric techniques that utilize the formation of charge transfer complexes between four antidepressant compounds, namely, amitriptyline hydrochloride (AMI), imipramine hydrochloride (IMI), clomipramine hydrochloride (CLO), and trimipramine maleate (TRI) acting as electron donors and two p-benzoquinones, namely, p-chloranilic acid (pCA) and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), serving as electron acceptors. The stoichiometry of the compounds produced exhibited a consistent 1:1 ratio in all instances, as established by Job’s method. Molar absorptivities, equilibrium association constants, and several other spectroscopic properties were determined for all complexes. The developed spectrophotometric techniques were validated intra-laboratory and successfully applied for quantitative assessment of the four antidepressant active ingredients in several commercial pharmaceutical formulations. The methods are relatively simple, fast, and use readily available laboratory instrumentation, making them easily applicable by most quality control laboratories worldwide.

show abstract

“…Many analytical procedures have been described to quantified antidepressant drugs in biological matrices down to the ng/mL level [ 2 ]. Gas chromatography with conventional and mass spectrometry (MS) detection [ 3 ], or liquid chromatography with diode array and mass spectrometry detection, being the most commonly used ones [ 4 , 5 ]. Ion mobility spectrometry (IMS) [ 6 ] has also gained interest for the direct analysis of antidepressant drugs.…”

Section: Introductionmentioning

confidence: 99%

Effects of the LC mobile phase in vacuum differential mobility spectrometry-mass spectrometry for the selective analysis of antidepressant drugs in human plasma

et al. 2022

View full text Add to dashboard Cite

The effect of LC mobile phase composition and flow rate (2–50 µL/min) on mobility behavior in vacuum differential mobility spectrometry (vDMS) was investigated for electrosprayed isobaric antidepressant drugs (AD); amitriptyline, maprotiline, venlafaxine; and structurally related antidepressants nortriptyline, imipramine, and desipramine. While at 2 µL/min, no difference in compensation voltage was observed with methanol and acetonitrile, at 50 µL/min, acetonitrile used for LC elution of analytes enabled the selectivity of the mobility separation to be improved. An accurate and sensitive method could be developed for the quantification of six AD drugs in human plasma using trap/elute micro-LC setup hyphenated to vDMS with mass spectrometric detection in the selected ion monitoring mode. The assay was found to be linear over three orders of magnitude, and the limit of quantification was of 25 ng/mL for all analytes. The LC-vDMS-SIM/MS method was compared to a LC-MRM/MS method, and in both cases, inter-assay precisions were lower than 12.5 and accuracies were in the range 91.5–110%, but with a four times reduced analysis time (2 min) for the LC-vDMS-SIM/MS method. This work illustrates that with vDMS, the LC mobile phase composition can be used to tune the ion mobility separation and to improve assay selectivity without additional hardware. Graphical abstract

show abstract

Applications of Gas Chromatography for the Analysis of Tricyclic Antidepressants in Biological Matrices

Cited by 8 publications

References 81 publications

Recent Applications of Gas Chromatography in Bioanalysis

Recent Applications of Gas Chromatography in Bioanalysis

Development of Visible Spectrophotometric Methods for the Determination of Tricyclic Antidepressants Based on Formation of Molecular Complexes with p-Benzoquinones

Effects of the LC mobile phase in vacuum differential mobility spectrometry-mass spectrometry for the selective analysis of antidepressant drugs in human plasma

Contact Info

Product

Resources

About