Applications of graphene oxide in case of nanomedicines and nanocarriers for biomolecules: review study

Mousavi, Seyyed Mojtaba; Hashemi, Seyyed Alireza; Ghasemi, Younes; Amani, Ali Mohammad; Babapoor, Aziz; Arjmand, Omid

doi:10.1080/03602532.2018.1522328

Cited by 81 publications

(55 citation statements)

References 171 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…GO is obtained by chemical oxidation of graphite and successive exfoliation of graphite oxide via ultrasonication. Oxygen functionality groups on GO surfaces are widely exploited in the chemical functionalization of GO, especially by esterification/amidation reactions at the carboxylic groups [ 21 , 22 , 23 ]. Processability and water stability due to ionizable groups on GO surfaces are the main advantages in the use of GO; whereas the structural defects on the sp 2 network and the lacking electrical conductivity are the main limits for GO applications as an electronic device [ 24 ].…”

Section: Graphene Nanomaterialsmentioning

confidence: 99%

Graphene-Based Strategies in Liquid Biopsy and in Viral Diseases Diagnosis

et al. 2020

View full text Add to dashboard Cite

Graphene-based materials are intriguing nanomaterials with applications ranging from nanotechnology-related devices to drug delivery systems and biosensing. Multifunctional graphene platforms were proposed for the detection of several typical biomarkers (i.e., circulating tumor cells, exosomes, circulating nucleic acids, etc.) in liquid biopsy, and numerous methods, including optical, electrochemical, surface-enhanced Raman scattering (SERS), etc., have been developed for their detection. Due to the massive advancements in biology, material chemistry, and analytical technology, it is necessary to review the progress in this field from both medical and chemical sides. Liquid biopsy is considered a revolutionary technique that is opening unexpected perspectives in the early diagnosis and, in therapy monitoring, severe diseases, including cancer, metabolic syndrome, autoimmune, and neurodegenerative disorders. Although nanotechnology based on graphene has been poorly applied for the rapid diagnosis of viral diseases, the extraordinary properties of graphene (i.e., high electronic conductivity, large specific area, and surface functionalization) can be also exploited for the diagnosis of emerging viral diseases, such as the coronavirus disease 2019 (COVID-19). This review aimed to provide a comprehensive and in-depth summarization of the contribution of graphene-based nanomaterials in liquid biopsy, discussing the remaining challenges and the future trend; moreover, the paper gave the first look at the potentiality of graphene in COVID-19 diagnosis.

show abstract

Section: Graphene Nanomaterialsmentioning

confidence: 99%

Graphene-Based Strategies in Liquid Biopsy and in Viral Diseases Diagnosis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In recent years, nano-quenchers have been developed in biosensing. Graphene oxide (GO) has a large surface-to-volume ratio and contains hydrophobic hexagonal rings that can interact with biomolecules (Chen et al, 2017;Mousavi et al, 2019). It is a good biosensor fluorescence quenching material.…”

Section: Introductionmentioning

confidence: 99%

Detection of Single Nucleotide Polymorphisms by Fluorescence Embedded Dye SYBR Green I Based on Graphene Oxide

Xia

Jing

et al. 2021

Front. Chem.

View full text Add to dashboard Cite

The detection of single nucleotide polymorphisms (SNPs) is of great significance in the early diagnosis of diseases and the rational use of drugs. Thus, a novel biosensor based on the quenching effect of fluorescence-embedded SYBR Green I (SG) dye and graphene oxide (GO) was introduced in this study. The probe DNA forms a double helix structure with perfectly complementary DNA (pcDNA) and 15 single-base mismatch DNA (smDNA) respectively. SG is highly intercalated with perfectly complementary dsDNA (pc-dsDNA) and exhibits strong fluorescence emission. Single-base mismatch dsDNA (SNPs) has a loose double-stranded structure and exhibits poor SG intercalation and low fluorescence sensing. At this time, the sensor still showed poor SNP discrimination. GO has a strong effect on single-stranded DNA (ssDNA), which can reduce the fluorescence response of probe DNA and eliminate background interference. And competitively combined with ssDNA in SNPs, quenching the fluorescence of SG/SNP, while the fluorescence value of pc-dsDNA was retained, increasing the signal-to-noise ratio. At this time, the sensor has obtained excellent SNP resolution. Different SNPs detect different intensities of fluorescence in the near-infrared region to evaluate the sensor's identification of SNPs. The experimental parameters such as incubation time, incubation temperature and salt concentration were optimized. Under optimal conditions, 1 nM DNA with 0–10 nM linear range and differentiate 5% SNP were achieved. The detection method does not require labeling, is low cost, simple in operation, exhibits high SNP discrimination and can be distinguished by SNP at room temperature.

show abstract

“…Hence, the anti-angiogenic action of GO can be very effective to fight cancer. To note, there are plenty of examples in the literature on the development of GO and modified GO platforms for anti-cancer therapy [ 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Angiogenic and Anti-Proliferative Graphene Oxide Nanosheets for Tumor Cell Therapy

Verde

Longo

Cucci

et al. 2020

IJMS

View full text Add to dashboard Cite

Graphene oxide (GO) is a bidimensional novel material that exhibits high biocompatibility and angiogenic properties, mostly related to the intracellular formation of reactive oxygen species (ROS). In this work, we set up an experimental methodology for the fabrication of GO@peptide hybrids by the immobilization, via irreversible physical adsorption, of the Ac-(GHHPH)4-NH2 peptide sequence, known to mimic the anti-angiogenic domain of the histidine-proline-rich glycoprotein (HPRG). The anti-proliferative capability of the graphene-peptide hybrids were tested in vitro by viability assays on prostate cancer cells (PC-3 line), human neuroblastoma (SH-SY5Y), and human retinal endothelial cells (primary HREC). The anti-angiogenic response of the two cellular models of angiogenesis, namely endothelial and prostate cancer cells, was scrutinized by prostaglandin E2 (PGE2) release and wound scratch assays, to correlate the activation of inflammatory response upon the cell treatments with the GO@peptide nanocomposites to the cell migration processes. Results showed that the GO@peptide nanoassemblies not only effectively induced toxicity in the prostate cancer cells, but also strongly blocked the cell migration and inhibited the prostaglandin-mediated inflammatory process both in PC-3 and in HRECs. Moreover, the cytotoxic mechanism and the internalization efficiency of the theranostic nanoplatforms, investigated by mitochondrial ROS production analyses and confocal microscopy imaging, unraveled a dose-dependent manifold mechanism of action performed by the hybrid nanoassemblies against the PC-3 cells, with the detection of the GO-characteristic cell wrapping and mitochondrial perturbation. The obtained results pointed out to the very promising potential of the synthetized graphene-based hybrids for cancer therapy.

show abstract

Applications of graphene oxide in case of nanomedicines and nanocarriers for biomolecules: review study

Cited by 81 publications

References 171 publications

Graphene-Based Strategies in Liquid Biopsy and in Viral Diseases Diagnosis

Graphene-Based Strategies in Liquid Biopsy and in Viral Diseases Diagnosis

Detection of Single Nucleotide Polymorphisms by Fluorescence Embedded Dye SYBR Green I Based on Graphene Oxide

Anti-Angiogenic and Anti-Proliferative Graphene Oxide Nanosheets for Tumor Cell Therapy

Contact Info

Product

Resources

About