Applications of immunoassay to pesticide analysis

Kaufman, B.

doi:10.1007/978-1-4613-1117-1_5

Progress in Food Contaminant Analysis 1996

DOI: 10.1007/978-1-4613-1117-1_5

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Applications of immunoassay to pesticide analysis

B. Kaufman¹

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Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: <I>Hydrogel Immunoassay</I>

Thomas

El-Giar

Locascio

et al.

Microfluidic Techniques

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The integration of immunoassays in microfluidic devices is a rapidly developing research area combining the power of immunoassays with the inherent benefits of microfluidics. Here, a general overview of microfluidic-based immunoassays is presented along with a method for immobilizing antibodies in polyacrylamide gel plugs set in microfluidic channels. These antigen-specific hydrogels can be rapidly formed by photopolymerizing monomer solutions mixed with antibodies or other large proteins. The resulting antigen-specific hydrogels contain pore sizes appropriate for physical entrapment of large antibodies while remaining permeable to smaller proteins. The open structure of these hydrogels enables the capture and concentration of target antigens present at low concentrations. Such physical entrapment provides a conceptually simple method of immobilization compared with immobilization of proteins on surfaces and offers advantages such as resistance to chemical and thermal denaturation.

show abstract

Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: <I>Hydrogel Immunoassay</I>

Thomas

El-Giar

Locascio

et al.

Microfluidic Techniques

View full text Add to dashboard Cite

show abstract

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Applications of immunoassay to pesticide analysis

Cited by 1 publication

References 147 publications

Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: <I>Hydrogel Immunoassay</I>

Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: <I>Hydrogel Immunoassay</I>

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