Applications of infrared spectroscopy to biology and medicine

Mantsch, Henry H.; McElhaney, Ronald N.

doi:10.1016/0022-2860(90)80374-s

Cited by 27 publications

(13 citation statements)

References 11 publications

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“…Frequency changes of the antisymmetric and symmetric CH2 stretching (w,CH2 and w,CH2) bands on the monolayer compression are shown in Table 2. Both bands shift to lower frequencies with the increase in the surface pressure, suggesting the increase in the amount of all-trans conformers of the hydrocarbon chain (14)(15)(16). These frequency shifts are very similar to the recent results of ITIR external reflection spectroscopy by Dluhy et al (17,18) for DPPC monolayers spread on the water surface.…”

Section: Resultssupporting

confidence: 87%

Molecular orientation in thin Langmuir–Blodgett films of dipalmitoylphosphatidylcholine as studied by FTIR transmission and reflection–absorption spectroscopy

Okamura¹,

Umemura²,

Takenaka³

1991

Can. J. Chem.

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EMIKO OKAMURA, JUNZO UMEMURA, and TOHRU TAKENAKA. Can. J. Chem. 69,1691Chem. 69, (1991.Langmuir-Blodgett (LB) films of DPPC were prepared by the horizontal lifting technique at various surface pressures encompassing the plateau region of its surface pressure (a) -area (A) isotherm. Molecular orientation in the LB films was quantitatively evaluated by a method recently proposed by Umemura et al. (J. Phys. Chem. 94, 62 (1990)) using Fourier transform infrared (FTIR) transmission and reflection-absorption (RA) spectra. From the results obtained, it is apparent that (i) in the LB films deposited at higher surface pressures above the plateau, the hydrocarbon chains are of all-trans conformation and well oriented at the tilting angle of ca. 20" from the surface normal; (ii) at lower surface pressures below the plateau, the chain segments are almost randomly oriented (like in a liquid-crystalline state) with an increased amount of the gauche conformers; and (iii) in the films deposited within the plateau, the hydrocarbon chains are in an intermediate state between the above two states. It is therefore concluded that the plateau corresponds to a process of the reorientation of the hydrocarbon chains from an almost randomly-oriented state to a highly-oriented one with the increase in the number of the all-trans conformers.Key words: dipalmitoylphosphatidylcholine, Langmuir-Blodgett film, molecular orientation, infrared reflection-absorption spectra, surface pressure-area isotherm.EMIKO OKAMURA, JUNZO UMEMURA et TOHRU TAKENAKA. Can. J. Chem. 69,1691Chem. 69, (1991.Utilisant la technique du soulkvement horizontal a diverse pressions superficielles couvrant la region du plateau de son isotherme de la pression superficielle (a) -surface (A), on a prCparC des films de Langmuir-Blodgett (LB) de la DPPC. Utilisant la mCthode proposCe rCcernrnent par Umemura et ses collaborateurs (J. Phys. Chem. 94, 62 (1990)) et bade sur les spectres infrarouges par transformation de Fourier de transmission (IRTF) et de rkflexion-absorption (RA), on a CvaluC d'une faqon quantitative l'orientation molCculaire dans les films de LB. A partir des rksultats obtenus, il est apparent que, dans les films LB dCposCs : (i) a des pressions superficielles supkrieures a celle du plateau, les chaines hydrocarbonCes posskdent toutes la conformation complbtement trans et elles sont toutes orientCes un angle d'environ 20°par rapport &la surface normale; (ii) a des pressions infkrieures a celles de la pression du plateau, les segments de chaines sont pratiquement orient& au hasard (Ctat semblable a celui d'un cristal-liquide) avec une quantitk plus importante des conformkres gauches; et (iii) a des pressions correspondant a celles du plateau, les chaines hydrocarbonCes sont dans un Ctat intermkdiaire entre les deux Ctats mentionnks plus haut. On en conclut donc que le plateau correspond a un processus de rkorientation des chaines hydrocarbontes d'un Ctat orient6 pratiquement au hasard vers un Ctat hautement orient6 avec une augmentation du nombre des conformbr...

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Section: Resultssupporting

confidence: 87%

Molecular orientation in thin Langmuir–Blodgett films of dipalmitoylphosphatidylcholine as studied by FTIR transmission and reflection–absorption spectroscopy

Okamura¹,

Umemura²,

Takenaka³

1991

Can. J. Chem.

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“…The amide II bands usually arise from the δ(N-H) bending vibrations coupled to ν(C-N) stretching vibrations. Namely, it is known that the amide I and II bands are present in the spectral 1500-1700 cm −1 range for protein molecules, first of all collagen [16,19]. In secondary amines, one absorption band is seen, arising from the stretching vibrations of NH group, of a medium to weak intensity, at 3350-3300 cm −1 or 3450 cm −1 [33].…”

Section: Resultsmentioning

confidence: 99%

“…According to the given FT-IR spectrum analysis of the protein structures, it is necessary to observe first of all the absorption bands arising from the ν(C=O) stretching vibrations, so-called amide I bands, which appear at about 1650 cm −1 [16,19]. Generally, amide I bands originate from the ν(C=O) stretching vibrations coupled to δ(N-H) bending vibrations.…”

Section: Resultsmentioning

confidence: 99%

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Molecular spectroscopy analysis of the substitution of bone tissue by HAp/PLLA composite biomaterial

Ignjatović

Uskoković

2004

Journal of Spectroscopy

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Due to its pronounced osteoinductive properties, calcium hydroxyapatite (HAp) has been widely used in medicine. Bioresorptive poly-L-lactide (PLLA) as a polymer biomaterial has been also used extensively in medicine for its non-toxicity and biocompatibility. To combine the advantages exhibited by each of these materials, a HAp/PLLA composite biomaterial has been synthesized and used for reconstruction and repair of bone defects. Hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial with PLLA of 50,000 and 430,000 g/mole molecular weight was studiedin vivo. The biocomposite with PLLA of both molecular weights was implanted into mice, then removed from their organisms and analyzed by the Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and histopathologic analysis. Characteristic absorption bands, registered and defined by FT-IR spectroscopy, confirm the formation of new functional groups and compounds during the bone repair process using HAp/PLLA biocomposite with PLLA of 50,000 and 430,000 molecular weights. Analysis of the microstructures of the sample surfaces by scanning electron microscopy (SEM) before and after implantation revealed bioresorption of the PLLA polymer phase in the system with PLLA of lower molecular weight and generation of collagen fibers at the sites of implanted bioresorptive PLLA. As the studied synthetic materials behave as the natural bone, i.e., they are phagocytosed and resorpable, they can be considered as biocompatible.

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“…To date FT-IR spectroscopy has proven to be a powerful analytical tool to probe the structure and confirmation of lipids, proteins and nucleic acids 3 and also of complex biological materials such as tissues, body fluids and cell cultures 4,5 . The ability of FT-IR spectroscopy to detect changes in the chemical composition and morphology of cells led to its use in distinguishing differences among cell populations; for example, between normal and cancerous cells 6,7 as well as characterisation of cancerous state using proliferative cell lines at various stages of the cell cycle 8 .…”

Section: Introductionmentioning

confidence: 99%

FT-Infrared Spectroscopic Studies of Lymphoma, Lymphoid and Myeloid Leukaemia Cell Lines

Babrah

McCarthy

Lush

et al. 2007

Diagnostic Optical Spectroscopy in Biomedicine IV

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This paper presents a novel method to characterise spectral differences that distinguish leukaemia and lymphoma cell lines. This is based on objective spectral measurements of major cellular biochemical constituents and multivariate spectral processing. Fourier transform infrared (FT-IR) maps of the lymphoma, lymphoid and myeloid leukaemia cell samples were obtained using a Perkin-Elmer Spotlight 300 FT-IR imaging spectrometer. Multivariate statistical techniques incorporating principal component analysis (PCA) and linear discriminant analysis (LDA) were used to construct a mathematical model. This model was validated for reproducibility. Multivariate statistical analysis of FTIR spectra collected for each cell sample permit a combination of unsupervised and supervised methods of distinguishing cell line types. This resulted in the clustering of cell line populations, indicating distinct bio-molecular differences. Major spectral differences were observed in the 4000 to 800 cm-1 spectral region. Bands in the averaged spectra for the cell line were assigned to the major biochemical constituents including; proteins, fatty acids, carbohydrates and nucleic acids. The combination of FT-IR spectroscopy and multivariate statistical analysis provides an important insight into the fundamental spectral differences between the cell lines, which differ according to the cellular biochemical composition. These spectral differences can serve as potential biomarkers for the differentiation of leukaemia and lymphoma cells. Consequently these differences could be used as the basis for developing a spectral method for the detection and identification of haematological malignancies.

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Applications of infrared spectroscopy to biology and medicine

Cited by 27 publications

References 11 publications

Molecular orientation in thin Langmuir–Blodgett films of dipalmitoylphosphatidylcholine as studied by FTIR transmission and reflection–absorption spectroscopy

Molecular orientation in thin Langmuir–Blodgett films of dipalmitoylphosphatidylcholine as studied by FTIR transmission and reflection–absorption spectroscopy

Molecular spectroscopy analysis of the substitution of bone tissue by HAp/PLLA composite biomaterial

FT-Infrared Spectroscopic Studies of Lymphoma, Lymphoid and Myeloid Leukaemia Cell Lines

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