Applications of Ionic Liquids to Increase the Efficiency of Lipase Biocatalysis

Péter, Fráncisc; Paul, Cristina; Ursoiu, A.

doi:10.5772/15331

Cited by 2 publications

(6 citation statements)

References 43 publications

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“…In this order, different amounts of lipase B from Candida antarctica B were immobilized by sol-gel entrapment or by entrapment combined with adsorption on Celite 545 [ 19 ]. The sol-gel matrix was obtained using a ternary precursor system with PhTMOS, MeTMOS and TMOS at 1.6:0.4:1 molar ratio, and [Omim]BF 4 as an immobilization additive [ 17 ]. The immobilized lipases have been evaluated in the model reaction of enantioselective acylation of 2-octanol with vinyl acetate at 40 °C, in n -hexane.…”

Section: Resultsmentioning

confidence: 99%

“…Ionic liquids have also proved to be effective additives in the entrapment process. However, is difficult to identify their exact role in preparation of sol-gel materials and the influence of their presence during immobilization [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…Mesoporous silica supports were proven particularly efficient for applications related to retention and release of various biomolecules [ 15 ]. Sol-gel encapsulation has proved to be a particularly easy and effective way to immobilize purified enzymes and other proteins or even whole cells [ 1 , 3 , 14 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. The main advantages of this immobilization technique are enhanced operational and thermal stability of the obtained preparates.…”

Section: Introductionmentioning

confidence: 99%

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Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols

et al. 2012

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Sol-gel entrapment is an efficient immobilization technique that allows preparation of robust and highly stable biocatalysts. Lipase from Candida antarctica B was immobilized by sol-gel entrapment and by sol-gel entrapment combined with adsorption on Celite 545, using a ternary silane precursor system. After optimization of the immobilization protocol, the best enzyme loading was 17.4 mg/g support for sol-gel entrapped lipase and 10.7 mg/g support for samples obtained by entrapment and adsorption. Sol-gel immobilized enzymes showed excellent values of enantiomeric ratio E and activity when ionic liquid 1-octyl-3-methyl-imidazolium tetrafluoroborate was used as additive. Immobilization increased the stability of the obtained biocatalysts in several organic solvents. Excellent operational stability was obtained for the immobilized lipase, maintaining unaltered catalytic activity and enantioselectivity during 15 reuse cycles. The biocatalysts were characterized using scanning electron microscopy (SEM) and fluorescence microscopy. The improved catalytic efficiency of entrapped lipases recommends their application for large-scale kinetic resolution of optically active secondary alcohols.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols

et al. 2012

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“…Ionic liquids have also been proved to be efficient immobilization additives [ 24 , 25 , 26 ]. However, identifying the exact role of ionic liquids in the preparation of xerogels and the influence on the catalytic properties of the entrapped enzyme is not an easy task to do.…”

Section: Introductionmentioning

confidence: 99%

“…However, identifying the exact role of ionic liquids in the preparation of xerogels and the influence on the catalytic properties of the entrapped enzyme is not an easy task to do. Some advantages of ionic liquid addition during the sol–gel immobilization of the enzymes include their protection against inactivation by the released alcohol and the shrinkage of the gel during the maturation and drying step of the sol–gel immobilization process, an increased gelation time, and it having an influence on the gel structure (increase in the average pore radius and reduction in the pore size distribution) [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of the Catalytic Performance and Operational Stability of Sol-Gel-Entrapped Cellulase by Tailoring the Matrix Structure and Properties

Vasilescu

Suquet

Hulka

et al. 2022

Gels

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Commercial cellulase Cellic CTec2 was immobilized by the entrapment technique in sol–gel matrices, and sol–gel entrapment with deposition onto magnetic nanoparticles, using binary or ternary systems of silane precursors with alkyl- or aryl-trimethoxysilanes, at different molar ratios. Appropriate tailoring of the sol–gel matrix allowed for the enhancement of the catalytic efficiency of the cellulase biocatalyst, which was then evaluated in the hydrolysis reaction of Avicel microcrystalline cellulose. A correlation between the catalytic activity with the properties of the sol–gel matrix of the nanobiocatalysts was observed using several characterization methods: scanning electron microscopy (SEM), fluorescence microscopy (FM), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA/DTA). The homogeneous distribution of the enzymes in the sol–gel matrix and the mass loss profile as a function of temperature were highlighted. The influence of temperature and pH of the reaction medium on the catalytic performance of the nanobiocatalysts as well as the operational stability under optimized reaction conditions were also investigated; the immobilized biocatalysts proved their superiority in comparison to the native cellulase. The magnetic cellulase biocatalyst with the highest efficiency was reused in seven successive batch hydrolysis cycles of microcrystalline cellulose with remanent activity values that were over 40%, thus we obtained promising results for scaling-up the process.

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Applications of Ionic Liquids to Increase the Efficiency of Lipase Biocatalysis

Cited by 2 publications

References 43 publications

Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols

Sol-gel Entrapped Candida antarctica lipase B — A Biocatalyst with Excellent Stability for Kinetic Resolution of Secondary Alcohols

Enhancement of the Catalytic Performance and Operational Stability of Sol-Gel-Entrapped Cellulase by Tailoring the Matrix Structure and Properties

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