Applications of low-intensity pulsed ultrasound to increase monoclonal antibody production in CHO cells using shake flasks or wavebags

Zhao, Yijie; Xing, Jida; Jin, Xing; Ang, Woon T.; Chen, Jie

doi:10.1016/j.ultras.2014.04.025

Cited by 18 publications

(16 citation statements)

References 18 publications

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“…Reports on the hormetic dose-response to antibiotics date back to the 1950s and various antibiotics were found to be able to cause hormetic effects [56], [57]. Low-intensity pulsed ultrasound (LIPUS) was also found to stimulate cell growth and antibody production, although high dose of ultrasound can kill cells [66]. Agents that can cause hormetic effects can be classified into two types: 1) chemical toxins that can leave residuals and accumulate in cells, such as Ag-NP and other antibiotics; 2) agents that can physically change cells and leave no residuals, such as LIPUS.…”

Section: Discussionmentioning

confidence: 99%

Contradictory effects of silver nanoparticles on activated sludge wastewater treatment

Sheng

Nostrand

Zhou

et al. 2018

Journal of Hazardous Materials

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Increased amount of nano-silver will be released into domestic and industrial waste streams due to its extensive application. However, great controversy still exists on the effects of silver nanoparticle (Ag-NP) on biological wastewater treatment processes and a toxicology model has not been built yet. Four sequencing batch reactors with activated sludge has been run for over three months with different silver species at a concentration of 1mg Ag/L in influent. Both freshly prepared Ag-NPs and aged Ag-NPs were tested with released silver ion as control. Results in this study showed that Ag-NPs, especially freshly prepared Ag-NPs, can help to maintain or even increase the diversity of microbial community in activated sludge and the biomass concentration even under long-term treatment. It indicates that the hormesis model need to be considered for the toxicology of Ag-NPs.

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Section: Discussionmentioning

confidence: 99%

Contradictory effects of silver nanoparticles on activated sludge wastewater treatment

Sheng

Nostrand

Zhou

et al. 2018

Journal of Hazardous Materials

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“…In this paper, we combined both physical and chemical approaches in the development of a high-efficient gene delivery method with low-cytotoxicity. From the previous work done in our group as well as by other groups and reported in the literature, we knew that LIPUS could transiently increase cell membrane permeability [39] as well as can be beneficial for cell viability [35,38]. Our goal was to evaluate whether ultrasound can enhance the entry of genes into the cells, when used with MNPs as a transfection tool.…”

Section: Resultsmentioning

confidence: 99%

“…Because different ultrasound parameters, such as wave intensity, treatment duration and frequency of the treatment, can have a significantly different effect on the cell growth and the membrane permeability [35,39] , we needed to select the optimal conditions of the LIPUS stimulation for our purpose among five different ultrasound intensities and durations (1.No LIPUS stimulation; 2. LIPUS at 30 mW/cm 2 for 5 minutes; 3.…”

Section: Selecting Optimal Ultrasound Conditionmentioning

confidence: 99%

“…It has been proven to be very safe for human use for many aspects of the medical applications, such as bone healing [28], inflammation inhibiting [29], soft-tissue regeneration [30], and the induction of cell-membrane porosity. LIPUS has also been proven to help division and proliferation in many types of cells, such as algal cell [39,40], stem/progenitor cell [37] and mammalian cell [38] and can also increase CHO cells growth and antibody production [35], increase cell permeability [39] and enhance gene delivery by using microbubble [36]. Again, the safe operational intensity range of LIPUS is between 0.02 and 1 W/cm 2 and treatment durations of 5 -20 minutes per day.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Magnetic Nanoparticle-Based Gene Delivery

Chen

Zhang

Abdelrasoul

et al. 2020

Preprint

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Low-intensity pulsed ultrasound (LIPUS), a special type of ultrasonic stimulation, is attracting a lot of attention for both clinical and scientific research. In this paper, we report a concept of a new method using magnetic nanoparticles (MNPs) for LIPUSassisted gene delivery. The MNPs are iron oxide superparamagnetic nanoparticles, coated with polyethyleneimine (PEI), which introduces a high positive surface charge, favorable for the binding of genetic material. Due to the paramagnetic properties of the MNPs, the application of an external magnetic field increases transfection efficiency; meanwhile, LIPUS stimulation enhances cell permeability. We found out that stimulation at the intensity of 30 mW/cm 2 for 10 minutes yields optimal results with a minimal adverse effect on the cells. Combining the effect of the external magnetic field and LIPUS, the genetic material (GFP or Cherry Red plasmid in our case) can enter the cells. The flow cytometry results showed that by using just a magnetic field to direct the genetic material, the transfection efficiency of HEK 293 cells that were treated with our MNPs was 56.1%. Coupled with LIPUS stimulation, it increased to 61.5% or 19% higher than the positive control (Lipofectamine 2000). In addition, compared with the positive control, our method showed less toxicity. Cell viability after transfection was 63.61%, 19% higher than with the standard transfection technique. In conclusion, we designed a new gene-delivery technique that is affordable, targeted, shows low-toxicity, yet high transfection efficiency, compared to other conventional approaches.

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“…Low intensity pulsed ultrasound (LIPUS) has shown great utility and promise in medical therapeutic treatments, including bone and soft tissue healing, tooth root resorption [ 1 , 2 , 3 , 4 ], stem cell proliferation and differentiation [ 5 , 6 ], and antibody production [ 7 , 8 , 9 ]. In these applications, 1.5 MHz has been validated to be an effective stimulation frequency, and the applied intensities are within a range between 30 mW/cm 2 and 100 mW/cm 2 .…”

Section: Introductionmentioning

confidence: 99%

Design of a Thermoacoustic Sensor for Low Intensity Ultrasound Measurements Based on an Artificial Neural Network

Xing

Chen

2015

Sensors

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In therapeutic ultrasound applications, accurate ultrasound output intensities are crucial because the physiological effects of therapeutic ultrasound are very sensitive to the intensity and duration of these applications. Although radiation force balance is a benchmark technique for measuring ultrasound intensity and power, it is costly, difficult to operate, and compromised by noise vibration. To overcome these limitations, the development of a low-cost, easy to operate, and vibration-resistant alternative device is necessary for rapid ultrasound intensity measurement. Therefore, we proposed and validated a novel two-layer thermoacoustic sensor using an artificial neural network technique to accurately measure low ultrasound intensities between 30 and 120 mW/cm2. The first layer of the sensor design is a cylindrical absorber made of plexiglass, followed by a second layer composed of polyurethane rubber with a high attenuation coefficient to absorb extra ultrasound energy. The sensor determined ultrasound intensities according to a temperature elevation induced by heat converted from incident acoustic energy. Compared with our previous one-layer sensor design, the new two-layer sensor enhanced the ultrasound absorption efficiency to provide more rapid and reliable measurements. Using a three-dimensional model in the K-wave toolbox, our simulation of the ultrasound propagation process demonstrated that the two-layer design is more efficient than the single layer design. We also integrated an artificial neural network algorithm to compensate for the large measurement offset. After obtaining multiple parameters of the sensor characteristics through calibration, the artificial neural network is built to correct temperature drifts and increase the reliability of our thermoacoustic measurements through iterative training about ten seconds. The performance of the artificial neural network method was validated through a series of experiments. Compared to our previous design, the new design reduced sensing time from 20 s to 12 s, and the sensor’s average error from 3.97 mW/cm2 to 1.31 mW/cm2 respectively.

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Applications of low-intensity pulsed ultrasound to increase monoclonal antibody production in CHO cells using shake flasks or wavebags

Cited by 18 publications

References 18 publications

Contradictory effects of silver nanoparticles on activated sludge wastewater treatment

Contradictory effects of silver nanoparticles on activated sludge wastewater treatment

Ultrasound-Assisted Magnetic Nanoparticle-Based Gene Delivery

Design of a Thermoacoustic Sensor for Low Intensity Ultrasound Measurements Based on an Artificial Neural Network

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