Applications of Magnetic Nanomaterials in Biosensors and Diagnostics

Altıntaş, Zeynep

doi:10.1002/9781119065036.ch12

Cited by 13 publications

(20 citation statements)

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“…The use of nanomaterials significantly enhanced the sensor signal and allows detecting trace amounts of pathogens that has critical role in diagnostic [ 11 ]. To obtain a more sensitive diagnostic assay for Salmonella detection we, therefore, used the AuNP-modified sandwich assay.…”

Section: Resultsmentioning

confidence: 99%

“…There are several methods used for the detection of Salmonella serotypes, such as cultivation techniques [ 6 ], enzyme-linked immunosorbent assays (ELISAs) [ 7 ], polymerase chain reaction (PCR) methods [ 8 , 9 , 10 ], and biosensors [ 11 ]. The gold standard for detection of Salmonella serotypes are still conventional methods that are sensitive and inexpensive.…”

Section: Introductionmentioning

confidence: 99%

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Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

et al. 2018

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Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL−1. The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

et al. 2018

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“…Some inorganic nanomaterials, such as Fe 3 O 4 , demonstrate a magnetic nature and can be easily manipulated by an external magnetic field, allowing simple extraction and buffer replacement, and also provide a high signal-to-noise ratio in biological samples, in addition to their large surface area [143,144,145,146]. They can be used for homogenising, trapping, enriching, transporting, and labelling of analytes, especially in POC testing.…”

Section: Applications Of Inorganic Nanomaterials In Healthcare Biomentioning

confidence: 99%

“…Depending on their synthesis mechanism, these particles may have varying size distributions and their sizes range from 10 nm to 1000 nm. MNPs are generally employed as either transducers, which may be electrochemical, piezoelectric, optical, or colorimetric in nature, or as labels in conjugation with biomolecules [146]. MNP biosensors are useful in various disciplines, including the food industry, medical diagnosis, and environmental investigations [177].…”

Section: Applications Of Inorganic Nanomaterials In Healthcare Biomentioning

confidence: 99%

Nanomaterials for Healthcare Biosensing Applications

Pirzada

Altıntaş

2019

Sensors

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185

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In recent years, an increasing number of nanomaterials have been explored for their applications in biomedical diagnostics, making their applications in healthcare biosensing a rapidly evolving field. Nanomaterials introduce versatility to the sensing platforms and may even allow mobility between different detection mechanisms. The prospect of a combination of different nanomaterials allows an exploitation of their synergistic additive and novel properties for sensor development. This paper covers more than 290 research works since 2015, elaborating the diverse roles played by various nanomaterials in the biosensing field. Hence, we provide a comprehensive review of the healthcare sensing applications of nanomaterials, covering carbon allotrope-based, inorganic, and organic nanomaterials. These sensing systems are able to detect a wide variety of clinically relevant molecules, like nucleic acids, viruses, bacteria, cancer antigens, pharmaceuticals and narcotic drugs, toxins, contaminants, as well as entire cells in various sensing media, ranging from buffers to more complex environments such as urine, blood or sputum. Thus, the latest advancements reviewed in this paper hold tremendous potential for the application of nanomaterials in the early screening of diseases and point-of-care testing.

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“…Although the use of carbon nanotubes, several metal nanoparticles, and polymers in biosensing has become quite common [20,21], limited work has been done in incorporating carbon dots (CDs) onto the enzymes. Very recently, CDs (nontoxic nanoparticles) were incorporated onto protein molecules to achieve unique properties.…”

Section: Introductionmentioning

confidence: 99%

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

Sharma

Mićić

et al. 2019

Molecules

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Nanoparticles have been conjugated to biological systems for numerous applications such as self-assembly, sensing, imaging, and therapy. Development of more reliable and robust biosensors that exhibit high response rate, increased detection limit, and enhanced useful lifetime is in high demand. We have developed a sensing platform by the conjugation of β-galactosidase, a crucial enzyme, with lab-synthesized gel-like carbon dots (CDs) which have high luminescence, photostability, and easy surface functionalization. We found that the conjugated enzyme exhibited higher stability towards temperature and pH changes in comparison to the native enzyme. This enriched property of the enzyme was distinctly used to develop a stable, reliable, robust biosensor. The detection limit of the biosensor was found to be 2.9 × 10−4 M, whereas its sensitivity was 0.81 µA·mmol−1·cm−2. Further, we used the Langmuir monolayer technique to understand the surface properties of the conjugated enzyme. It was found that the conjugate was highly stable at the air/subphase interface which additionally reinforces the suitability of the use of the conjugated enzyme for the biosensing applications.

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Applications of Magnetic Nanomaterials in Biosensors and Diagnostics

Cited by 13 publications

References 100 publications

Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

Nanomaterials for Healthcare Biosensing Applications

Conjugation of Carbon Dots with β-Galactosidase Enzyme: Surface Chemistry and Use in Biosensing

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