Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

Zerva, Anastasia; Simić, Slavko; Topakas, Evangelos; Nikodinovic-Runic, Jasmina

doi:10.3390/catal9121023

Cited by 94 publications

(72 citation statements)

References 180 publications

(197 reference statements)

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“…Despite the potential of laccase-based biosensors as a sensitive analytical tool for phenolic compound detection, the commercial/industrial implementation of those devices is not available. This limitation is mostly due to laccase high production costs associated with isolation and purification processes [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Electroanalytical Biosensor Based on Maramiellus colocasiae Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha

et al. 2021

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In this work, we developed an enzymatic voltammetric biosensor for the determination of catechin and gallic acid in green tea and kombucha samples. The differential pulse voltammetry (DPV) methodology was optimized regarding the amount of crude enzyme extract, incubation time in the presence of the substrates, optimal pH, reuse of the biosensor, and storage time. Samples of green tea and kombucha were purchased in local markets in the city of Goiânia-GO, Brazil. High performance liquid chromatography (HPLC) and Folin-Ciocalteu spectrophotometric techniques were performed for the comparison of the analytical methods employed. In addition, two calibration curves were made, one for catechin with a linear range from 1 to 60 µM (I = −0.152 * (catechin) − 1.846), with a detection limit of 0.12 µM and a quantification limit of 0.38 µM and one for gallic acid with a linear range from 3 to 60 µM (I = −0.0415 * (gallic acid) − 0.0572), with a detection limit of 0.14 µM and a quantification limit of 0.42 µM. The proposed biosensor was efficient in the determination of phenolic compounds in green tea.

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Section: Introductionmentioning

confidence: 99%

Enzymatic Electroanalytical Biosensor Based on Maramiellus colocasiae Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha

et al. 2021

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“…1.10.3.2) oxidize a great variety of aromatic compounds [ 1 , 2 ]. They can be applied for wide industrial applications: organic synthesis, biosensor construction, biofuel cells development, biomass valorization, and xenobiotic biodegradation [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. Typical laccases have four copper atoms, which form three different copper centers (type T1, T2 and T3) [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Yellow Laccase from Botrytis cinerea 241

Radveikienė

Vidžiūnaitė

Meškienė

et al. 2021

JoF

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Typical laccases have four copper atoms, which form three different copper centers, of which the T1 copper is responsible for the blue color of the enzyme and gives it a characteristic absorbance around 610 nm. Several laccases have unusual spectral properties and are referred to as yellow or white laccases. Only two yellow laccases from the Ascomycota phylum have been described previously, and only one amino acid sequence of those enzymes is available. A yellow laccase Bcl1 from Botrytis cinerea strain 241 has been identified, purified and characterized in this work. The enzyme appears to be a dimer with a molecular mass of 186 kDa. The gene encoding the Bcl1 protein has been cloned, and the sequence analysis shows that the yellow laccase Bcl1 is phylogenetically distinct from other known yellow laccases. In addition, a comparison of amino acid sequences, and 3D modeling shows that the Bcl1 laccase lacks a conservative tyrosine, which is responsible for absorption quenching at 610 nm in another yellow asco-laccase from Sclerotinia sclerotiorum. High thermostability, high salt tolerance, broad substrate specificity, and the ability to decolorize dyes without the mediators suggest that the Bcl1 laccase is a potential enzyme for various industrial applications.

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“…by authors in other topics. [12][13][14] In the present study, 174 homeopathy-related patents were found for the period analyzed. In an original way, the patents were analyzed by nationality and type of applicants.…”

Section: Wo2016011104 (A1) Gensco Lab Llc United Statesmentioning

confidence: 84%

International Patent Survey of Products and Processes Concerning Homeopathy

2020

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Background Homeopathy is a complementary medicine characterized by the use of diluted and potentized medicines. Innovations in this area are constantly being proposed in the relevant literature, such as scientific articles and patents. The objective of this study was to carry out a patent survey of homeopathic products and processes. Methods A free and international patent database, Espacenet, was used. The search was carried out using the keyword homeop*, with two approaches: (1) no date restrictions for the search and (2) a date limit for the publication years 2008 to 2018. The patents from the limited period were organized as depositor countries, ownerships and groups, including homeopathic formulations, equipment, packaging, production procedures, and analytical methods. Results Without date restriction, 601 patents were identified in the survey. Of these, 174 were related to homeopathy and published in the period 2008 to 2018. Technologies come mainly from the following countries: United States (55 patents), Russia (24), Germany (15), France (13), India (12), Ukraine (11), Brazil (6), and China (6). Among the ownerships, 69% of patent applications were by independent depositors, 23% by companies, 7.5% by universities, and 0.5% by company/university partnerships. New formulations represented 75.9% of technologies, whilst the others comprised 14.3% for equipment, 3.8% for drugs packaging, 3.8% for production procedures, and 2.2% for analytical methods. Conclusions The present review helps visualize the homeopathy-related patents published in recent years, as well as the main countries and researchers investing in the field of homeopathy.

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Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

Cited by 94 publications

References 180 publications

Enzymatic Electroanalytical Biosensor Based on Maramiellus colocasiae Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha

Enzymatic Electroanalytical Biosensor Based on Maramiellus colocasiae Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha

Characterization of a Yellow Laccase from Botrytis cinerea 241

International Patent Survey of Products and Processes Concerning Homeopathy

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