2022
DOI: 10.3390/ruminants2040032
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Applications of Microsatellites and Single Nucleotide Polymorphisms for the Genetic Characterization of Cattle and Small Ruminants: An Overview

Abstract: The status of genetic diversity, adaptation to climate change or the identification of genes associated with traits of interest in livestock populations has been a major concern for scientists in the last decades. Biotechnology has evolved continuously, offering new tools and methodologies to analyse the genomes of livestock species. Biochemical markers or protein polymorphisms were the tools used for population studies many years ago, but over the last three decades the methodologies available to analyse live… Show more

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Cited by 9 publications
(6 citation statements)
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“…The pairwise F ST value was statistically significant among the four populations, implying genetic structure differentiation between the farms. The differentiation in genetic makeup reflects the accumulation of variations in allelic frequencies, providing crucial insights into the evolutionary history, genetic drift, and selection of distinct populations [ 53 , 54 ]. However, we have no evidence to identify gene pool associations and geographic origin as known ecotypes, as no capture records exist for Siamese crocodile individuals.…”
Section: Discussionmentioning
confidence: 99%
“…The pairwise F ST value was statistically significant among the four populations, implying genetic structure differentiation between the farms. The differentiation in genetic makeup reflects the accumulation of variations in allelic frequencies, providing crucial insights into the evolutionary history, genetic drift, and selection of distinct populations [ 53 , 54 ]. However, we have no evidence to identify gene pool associations and geographic origin as known ecotypes, as no capture records exist for Siamese crocodile individuals.…”
Section: Discussionmentioning
confidence: 99%
“…Since 2015, SNPs have become the predominant molecular markers in assessment of genetic diversity in local farm animals. Commercial highthroughput arrays are now available for the majority of livestock species, with two major options: (i) Illumina's Infiniumi Select Microarray or Bead Chip, utilizing single nucleotide extension or allele-specific primer extension, and (ii) Affymetrix's Gene Chip or Axiom Array, employing molecular inversion probe hybridization (Cortes et al, 2022) [6] . In contrast to multiallelic markers, analysis of biallelic SNP markers can be practically fully automated.…”
Section: Single Nucleotide Polymorphism Analysismentioning
confidence: 99%
“…In recent years, the application of DNA microsatellite markers has proven extremely useful in elucidating the population structure and genetic diversity in many animals and plant species [32][33][34]. One of the weakness when using microsatellite markers in livestock species appears to be the different size of the alleles of the same microsatellite marker under different PCR conditions [35]. As a result, new microsatellite markers were additionally designed in the animal species to facilitate comparison between studies.…”
Section: Introductionmentioning
confidence: 99%