Applications of Mosquito Ecology for Successful Insect Transgenesis-Based Disease Prevention Programs

Scott, Thomas W.; Harrington, Laura C.; Knols, Bart G. J.; Takken, Willem

doi:10.1007/978-0-387-78225-6_13

Cited by 2 publications

(2 citation statements)

References 122 publications

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“…The success of genetic control strategies depends on the ability of released males to mate successfully with females in the local population. Consequently, a thorough knowledge of mosquito mating biology is required to determine baseline levels which genetically modified males will need to attain in order to be successful and the variables most important for evaluating success (Ferguson et al 2005, Scott et al 2008, Magori et al 2009, Ferguson et al 2010). …”

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confidence: 99%

Male Mating History and Body Size Influence Female Fecundity and Longevity of the Dengue Vector Aedes aegypti

2011

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Male reproductive success is dependent on insemination success and reproductive output. During mating, male mosquitoes transfer not just sperm, but also seminal fluid proteins that may have profound effects on mated female biology and behavior. In this study, we investigated the role of male body size and mating history on semen depletion, female longevity and reproductive success in Aedes aegypti L. Small and large males were mated in rapid succession with up to five females. Our results indicate that large males had greater mating capacity than small males. A reduction in fecundity by more than 50% was observed in females that were fourth to mate with small males in comparison to females that mated earlier in sequence. For females mated to large males, this reduction became evident for females that mated fifth in sequence. No loss of fertility (measured as hatch rate) was observed in females that were 3rd-5th in mating sequence compared to females mated to virgin males. When females were maintained on a low-quality (5% sucrose) diet, those mated to virgin males had a greater longevity compared to females mated third in sequence. We conclude that small males experience more rapid seminal depletion than large males, and discuss the role of semen depletion in the mated female. Our results contribute towards a better understanding of the complexity of Ae. aegypti mating biology and provide refined estimates of mating capacity for genetic control efforts.

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Male Mating History and Body Size Influence Female Fecundity and Longevity of the Dengue Vector Aedes aegypti

2011

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“…means to reduce vector populations (Benedict and Robinson 2003;Carvalho et al 2015). However, a comprehensive understanding of mosquito activity and mating behaviors is essential to maximize the efficacy of genetic control strategies (Scott et al 2008). Indeed, differences in mating time have been noted in a variety of agricultural pests between sterilized, lab-reared animals and their control-targeted wild populations (see Miyatake et al 2002;Matsumoto et al 2008).…”

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QTL Determining Diel Flight Activity in Male Culex pipiens Mosquitoes

Hickner

Mori

Rund

et al. 2019

Journal of Heredity

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Members of the Culex pipiens complex differ in physiological traits that facilitate their survival in diverse environments. Assortative mating within the complex occurs in some regions where autogenous (the ability to lay a batch of eggs without a blood meal) and anautogenous populations are sympatric, and differences in mating behaviors may be involved. For example, anautogenous populations mate in flight/swarms, while autogenous populations often mate at rest. Here, we characterized flight activity of males and found that anautogenous strain males were crepuscular, while autogenous strain males were crepuscular and nocturnal, with earlier activity onset times. We conducted quantitative trait locus (QTL) mapping to explore the genetic basis of circadian chronotype (crepuscular vs. crepuscular and nocturnal) and time of activity onset. One major-effect QTL was identified for chronotype, while 3 QTLs were identified for activity onset. The highest logarithm of the odds (LOD) score for the chronotype QTL coincides with a chromosome 3 marker that contains a 15-nucleotide indel within the coding region of the canonical clock gene, cryptochrome 2. Sequencing of this locus in 7 different strains showed that the C-terminus of CRY2 in the autogenous forms contain deletions not found in the anautogenous forms. Consequently, we monitored activity in constant darkness and found males from the anautogenous strain exhibited free running periods of ~24 h while those from the autogenous strain were ~22 h. This study provides novel insights into the genetic basis of flight behaviors that likely reflect adaptation to their distinct ecological niches.

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Applications of Mosquito Ecology for Successful Insect Transgenesis-Based Disease Prevention Programs

Cited by 2 publications

References 122 publications

Male Mating History and Body Size Influence Female Fecundity and Longevity of the Dengue Vector Aedes aegypti

Male Mating History and Body Size Influence Female Fecundity and Longevity of the Dengue Vector Aedes aegypti

QTL Determining Diel Flight Activity in Male Culex pipiens Mosquitoes

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