Applications of Myostatin (MSTN) Gene in the Livestock Animals and Humans: A Review

Ahad, Wani A.; Andrabi, Mudasir; Beigh, Sajad A.; Bhat, Rumase A.; Shah, Rehan Ali

doi:10.20546/ijcmas.2017.609.222

Cited by 13 publications

(10 citation statements)

References 7 publications

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“…Identification of MSTN gene polymorphism and its association with growth traits will provide convenience for breeders to select individual livestock that are considered superior so that they can assist livestock producers in developing breeding strategies to optimize livestock potential. Through various approaches, the exploitation of MSTN gene mutations can provide significant benefits for several livestock industries (Ahad et al, 2017).…”

Section: Genotyping Of Mstn Genementioning

confidence: 99%

Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle

Daulay

Ningtias²,

Sumantri

et al. 2022

BuletinPeternak

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Investigating Myostatin (MSTN) as a potent inhibitor of skeletal muscle growth and development to produce excessive muscles is extremely essential for livestock breeding. This study aimed to analyze the expression of the MSTN gene and its relationships with genotype and phenotype (normal-muscled vs double-muscled) of Belgian Blue (BB) x Ongole Grade (PO) crossbred cattle. For that purpose, 12 animals from BB, PO, BB x PO F1, and BB x PO F2 cattle (3 animals each) raised at Balai Embrio Ternak (BET) Cipelang Bogor, West Java were used for blood sample collection. Genotyping analysis was performed using the PCR-RFLP method withprimer F: 5’-CTC TTC TTT CCT TTC CAT ACA GAC-3’ and R: 5’-AGG GGA AGA CCT TCC ATG TT-3’, while the MSTN gene expression was analyzed using the qPCR technique. As results, three genotypes: del.11/del.11, +/del.11, and +/+ were detected. The del.11/del.11 genotype, which showed a double-muscled phenotype was found in BB cattle and BB x PO F2 cattle. The +/del.11 genotype was found in BB x PO F1 cattle and BB x PO F2 cattle. The +/+ genotype, which showed a normal phenotype was only detected in PO cattle. There was a significant difference of the MSTN gene expression in the sampled animals among genotypes and between phenotypes (normal-muscled vs double muscled). The MSTN expression in animals with del.11/del.11 genotype was higher than that in animals with +/del11 and +/+ genotypes (P<0.05). Animals with +/+ genotype showed the lowest MSTN expression. It was concluded that double-muscled animals showed higher MSTN expression than normal-muscled animals.

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Section: Genotyping Of Mstn Genementioning

confidence: 99%

Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle

Daulay

Ningtias²,

Sumantri

et al. 2022

BuletinPeternak

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“…However, the missing of MSTN or gene homozygous mutation result in abnormal accumulation of muscle mass and proliferation of myofibers ( 62 ). In the research field of skeletal muscle growth and development, MSTN is recognized as a negative regulator with important physiological functions ( 63 ). GAA and its metabolite Cr have the effect of down-regulating the expression of MSTN and eliminating its inhibitory effect on muscle growth ( 64 ).…”

Section: The Regulation Of Guanidinoacetic Acid On Skeletal Muscle Developmentmentioning

confidence: 99%

Regulative Mechanism of Guanidinoacetic Acid on Skeletal Muscle Development and Its Application Prospects in Animal Husbandry: A Review

Yan

Liu

et al. 2021

Front. Nutr.

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Guanidinoacetic acid is the direct precursor of creatine and its phosphorylated derivative phosphocreatine in the body. It is a safe nutritional supplement that can be used to promote muscle growth and development. Improving the growth performance of livestock and poultry and meat quality is the eternal goal of the animal husbandry, and it is also the common demand of today's society and consumers. A large number of experimental studies have shown that guanidinoacetic acid could improve the growth performance of animals, promote muscle development and improve the health of animals. However, the mechanism of how it affects muscle development needs to be further elucidated. This article discusses the physical and chemical properties of guanidinoacetic acid and its synthesis pathway, explores its mechanism of how it promotes muscle development and growth, and also classifies and summarizes the impact of its application in animal husbandry, providing a scientific basis for this application. In addition, this article also proposes future directions for the development of this substance.

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“…MSTN is highly expressed in skeletal muscle, but lowly expressed in adipose tissue and cardiac muscle ( McPherron et al, 1997 ; Sharma et al, 1999 ). MSTN strongly affects skeletal muscle development ( McPherron and Lee, 1997 ; Zhao et al, 2005 ; Guo et al, 2009 ; Ahad et al, 2017 ). The MSTN mutation lead to a double muscling phenotype in cattle ( Grobet et al, 1997 ; McPherron and Lee, 1997 ), mice ( Bünger et al, 2004 ), sheep ( Clop et al, 2006 ) and other animals ( Schuelke et al, 2004 ; Mosher et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Viscera Characteristics of MSTN-Edited Heterozygous Pigs

Pei

Fan²,

Song³

et al. 2022

Front. Genet.

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Myostatin (MSTN) is a protein that negatively regulates growth of skeletal muscle, and inactivation of MSTN improves the mass of skeletal muscle. Our previous work found that MSTN+/− pigs have higher muscle depth and lower fat depth compared to wild type without any developmental problems. Therefore, MSTN-edited pigs are most likely to appear as heterozygotes in the potential future market, but the characteristics of organs in digestive and reproductive system of pigs with MSTN gene editing remains unclear. Here, we investigated the histological of the organs in the digestive system and reproductive system in MSTN gene heterozygotes at adult stages. The length of intestine was further compared between adult heterozygous and wild type pigs. We found no significant differences in histomorphology of organs, including heart, duodenum, jejunum, ileum, cecum, colon, testis, epididymis, ovaries, oviducts and uterus, between individuals from two genotypes. Moreover, there was no significant difference in the average length of intestine in adult pigs. Our data provide a reference for further clarifying the applications of MSTN gene edited pigs.

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Applications of Myostatin (MSTN) Gene in the Livestock Animals and Humans: A Review

Cited by 13 publications

References 7 publications

Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle

Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle

Regulative Mechanism of Guanidinoacetic Acid on Skeletal Muscle Development and Its Application Prospects in Animal Husbandry: A Review

Viscera Characteristics of MSTN-Edited Heterozygous Pigs

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