Applications of NMR spectroscopy based metabolomics: a review

Abstract

Metabolomics is the study which detects the changes of metabolites level. Metabolomics is a terminal view of the biological system. The end products of the metabolism, metabolites, reflect the responses to external environment. Therefore metabolomics gives the additional information about understanding the metabolic pathways. These metabolites can be used as biomarkers that indicate the disease or external stresses such as exposure to toxicant. Many kinds of biological samples are used in metabolomics, for example, cell, tissue, and bio fluids. NMR spectroscopy is one of the tools of metabolomics. NMR data are analyzed by multivariate statistical analysis and target profiling technique. Recently, NMR-based metabolomics is a growing field in various studies such as disease diagnosis, forensic science, and toxicity assessment.

Protective Effect of Ocotillol, the Derivate of Ocotillol-Type Saponins in Panax Genus, against Acetic Acid-Induced Gastric Ulcer in Rats Based on Untargeted Metabolomics

“…Ocotillol is also the major metabolite of ocotillol-type saponins after oral administration [14]. It was also reported to exert an anti-inflammatory effect and ameliorate 2,4,6-trinitro-benzenesulfonic acid-induced colitis [15][16][17]. While there was no report on the gastroprotective effect of ocotillol.…”

Metabolic Changes in Serum Metabolome of Beagle Dogs Fed Black Ginseng

“…In this study, high-resolution magic angle spinning (HR-MAS) NMR spectroscopy was used for metabolomics study. NMR spectroscopy requires little pretreatment and provides high reproducibility [ 30 ]. HR-MAS NMR spectroscopy requires simple sample preparation and a lower amount of sample than liquid NMR spectroscopy.…”

1H-NMR metabolomics analysis of nutritional components from two kinds of freshwater fish brain extracts

“…Therefore it is a robust and reliable technique in metabolomics. 11,12 Recently, due to being a powerful and versatile tool, NMR based metabolomics have been highlighted in nutrition research. 9,10,13 The NMR based global metabolomics methods involved in food nutrition research usually produce high-dimensional and complex data sets.…”