Applications of Protein Hydrolysates in Biotechnology

Pasupuleti, Vijai K.; Holmes, Chris; Demain, Arnold L.

doi:10.1007/978-1-4020-6674-0_1

Cited by 44 publications

(49 citation statements)

References 20 publications

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“…We used the complex growth medium LB, which consisted of tryptone (10 g/liter), yeast extract (5 g/ liter), and NaCl (5 g/liter). Tryptone and yeast extract are both derived from complex sources and have been commonly used as ingredients of fermentation media (2). Tryptone is an enzymatic digest of casein and is rich in tryptophan, large and small peptides, and amino acids.…”

Section: Methodsmentioning

confidence: 99%

“…It has a rich content of amino acids, peptides, water-soluble vitamins, growth factors, trace elements, and diverse carbohydrates. A major carbohydrate of LB medium is the disaccharide trehalose (2,22).…”

Section: Methodsmentioning

confidence: 99%

“…Such growth media usually contain inexpensive complex carbon and nitrogen sources that allow good performance (1,2,3,4,5). Besides the complexity of growth media, other parameters, such as mixing efficiency and perturbation, are also understood to allow variability of local substrate concentrations, pH, temperature, and oxygen levels (6).…”

mentioning

confidence: 99%

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Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Puentes-Téllez

Hansen

Sørensen

et al. 2013

Appl Environ Microbiol

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bIn a study aiming to assess bacterial evolution in complex growth media, we evaluated the long-term adaptive response of Escherichia coli MC1000 in Luria-Bertani (LB) medium. Seven parallel populations were founded and followed over 150 days in sequential batch cultures under three different oxygen conditions (defined environments), and 19 evolved forms were isolated. The emergence of forms with enhanced fitness was evident in competition experiments of all evolved forms versus the ancestral strain. The evolved forms were then subjected to phenotypic and genomic analyses relative to the ancestor. Profound changes were found in their phenotypes as well as whole-genome sequences. Interestingly, considerable heterogeneity was found at the intrapopulational level. However, consistently occurring parallel adaptive responses were found across all populations. The evolved forms all contained a mutation in galR, a repressor of the galactose operon. Concomitantly, the new forms revealed enhanced growth on galactose as well as galactose-containing disaccharides. This response was likely driven by the LB medium.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Puentes-Téllez

Hansen

Sørensen

et al. 2013

Appl Environ Microbiol

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“…Lower temperatures (e.g., 27 to 55 °C) and a shorter period of the hydrolysis time (e.g., 4 to 8 h) are often desirable for the generation of peptides in the food industry [5]. After the hydrolysis, the product is evaporated, pasteurized, and spray dried.…”

Section: Introductionmentioning

confidence: 99%

Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance

Hou

Dai

et al. 2017

J Animal Sci Biotechnol

305

201

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Recent years have witnessed growing interest in the role of peptides in animal nutrition. Chemical, enzymatic, or microbial hydrolysis of proteins in animal by-products or plant-source feedstuffs before feeding is an attractive means of generating high-quality small or large peptides that have both nutritional and physiological or regulatory functions in livestock, poultry and fish. These peptides may also be formed from ingested proteins in the gastrointestinal tract, but the types of resultant peptides can vary greatly with the physiological conditions of the animals and the composition of the diets. In the small intestine, large peptides are hydrolyzed to small peptides, which are absorbed into enterocytes faster than free amino acids (AAs) to provide a more balanced pattern of AAs in the blood circulation. Some peptides of plant or animal sources also have antimicrobial, antioxidant, antihypertensive, and immunomodulatory activities. Those peptides which confer biological functions beyond their nutritional value are called bioactive peptides. They are usually 2–20 AA residues in length but may consist of >20 AA residues. Inclusion of some (e.g. 2–8%) animal-protein hydrolysates (e.g., porcine intestine, porcine mucosa, salmon viscera, or poultry tissue hydrolysates) or soybean protein hydrolysates in practical corn- and soybean meal-based diets can ensure desirable rates of growth performance and feed efficiency in weanling pigs, young calves, post-hatching poultry, and fish. Thus, protein hydrolysates hold promise in optimizing the nutrition of domestic and companion animals, as well as their health (particularly gut health) and well-being.

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“…The protein hydrolysate can be derived from various sources such as milk, casein, animal derived (meat, collage) and vegetable origin (wheat gluten, rice protein, pea protein, soy protein, etc.) [2]. The protein hydrolysates are used to improve the taste of food products.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Characterization of Biofield Energy Treated Hi Veg<sup>TM</sup> Acid Hydrolysate

Trivedi¹

2016

IJNFS

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Abstract:The hydrolysed vegetable proteins are acidic or enzymatic hydrolytic product of proteins derived from various sources such as milk, meat or vegetables. The current study was designed to evaluate the impact of biofield energy treatment on the various physicochemical and spectra properties of Hi Veg TM acid hydrolysate i.e. a hydrolysed vegetable protein. The Hi Veg TM acid hydrolysate sample was divided into two parts that served as control and treated sample. The treated sample was subjected to the biofield energy treatment and its properties were analysed using particle size analyser, X-ray diffraction (XRD), surface area analyser, UV-visible and infrared (FT-IR) spectroscopy, and thermogravimetric analysis. The results of various parameters were compared with the control (untreated) part. The XRD data showed the decrease in crystallite size of treated sample from 110.27 nm (control) to 79.26 nm. The particle size was also reduced in treated sample as 162.13 µm as compared to the control sample (168.27 µm). Moreover, the surface area analysis revealed the 63.79% increase in the surface area of the biofield treated sample as compared to the control. The UV-Vis spectra of both samples i.e. control and treated showed the absorbance at same wavelength. However, the FT-IR spectroscopy revealed the shifting in peaks corresponding to N-H, C-H, C=O, C-N, and C-S functional groups in the treated sample with respect to the control. The thermal analysis also revealed the alteration in degradation pattern along with increase in onset temperature of degradation and maximum degradation temperature in the treated sample as compared to the control. The overall data showed the impact of biofield energy treatment on the physicochemical and spectroscopic properties of the treated sample of Hi Veg TM acid hydrolysate. The biofield treated sample might show the improved solubility, wettability and thermal stability profile as compared to the control sample.

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Applications of Protein Hydrolysates in Biotechnology

Cited by 44 publications

References 20 publications

Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance

Physicochemical Characterization of Biofield Energy Treated Hi Veg<sup>TM</sup> Acid Hydrolysate

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Applications of Protein Hydrolysates in Biotechnology

Cited by 44 publications

References 20 publications

Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance

Physicochemical Characterization of Biofield Energy Treated Hi Veg&lt;sup&gt;TM&lt;/sup&gt; Acid Hydrolysate

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Physicochemical Characterization of Biofield Energy Treated Hi Veg<sup>TM</sup> Acid Hydrolysate