Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases

Li, Siting; Song, Guang; Bai, Yina; Song, Ning; Zhao, Jiuliang; Liu, Jian; Hu, Chaofan

doi:10.3389/fimmu.2021.645632

Cited by 19 publications

(16 citation statements)

References 133 publications

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“…The membrane-based protein array, which is known to be a powerful platform for simultaneously detecting the expression levels of 507 human target proteins, has been proposed as a clinically useful method to identify disease biomarkers for diagnosis, prognosis, prediction, and understanding of previously unknown mechanisms involved in certain diseases [9][10][11]. However, to date, no study has used protein-antibody array technology to identify biomarkers for pregnancy outcome after placement of rescue cerclage for acute cervical insufficiency.…”

Section: Introductionmentioning

confidence: 99%

Antibody microarray analysis of the amniotic fluid proteome for predicting the outcome of rescue cerclage in patients with cervical insufficiency

et al. 2021

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Little is known about the biomarkers that can identify patient candidates suitable for rescue cerclage procedure. The purpose of the study was to identify novel biomarkers in amniotic fluid (AF) that can predict the outcome of rescue cerclage in patients with cervical insufficiency by using an antibody microarray. This case-control study was conducted using AF samples collected from singleton pregnant women who underwent rescue cerclage following a diagnosis of cervical insufficiency (19–25 weeks). Patients were divided into case (n=20) and control (n=20) groups based on the occurrence of spontaneous preterm delivery (SPTD) at <34 weeks of gestation after cerclage placement. The AF proteomes were analyzed using an antibody microarray for biomarker discovery work. Ten candidate biomarkers of interest were validated by ELISA. Thirty-one of the molecules studied showed significant intergroup differences (≥ 2-fold change in signal intensity). Validation by ELISA confirmed significantly higher levels of APRIL, S100 A8/A9, TIMP-1, MIP-1α, and IL-8 in women who had SPTD at <34 weeks. Of these, AF S100 A8/A9 and TIMP-1 levels were independent of other potentially confounding factors (e.g., cervical dilatation). S100 A8/A9 had the highest area under the curve at 0.857. Using protein–antibody microarray technology, we identified differentially expressed proteins and several novel biomarkers (APRIL, IL-8, MIP-1α, S100 A8/A9, and TIMP-1) in AF from women who had SPTB at <34 weeks after cerclage for cervical insufficiency. These data can provide an insight into the molecular mechanisms underlying SPTD after rescue cerclage in patients with cervical insufficiency.

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Section: Introductionmentioning

confidence: 99%

Antibody microarray analysis of the amniotic fluid proteome for predicting the outcome of rescue cerclage in patients with cervical insufficiency

et al. 2021

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“…Peptide-based microarrays are similar to protein microarrays, but they have been constructed using thousands of pre-synthesized peptides on a microarray surface ( Li et al, 2021 ). Compared to proteins, synthesized peptides are more stable, easy to produce and modify, and more importantly, less expensive.…”

Section: Methodologies For Identification and Quantification Of Lenti...mentioning

confidence: 99%

“…The peptide-based microarray technique required a low amount of human serum sample and showed higher sensitivity compared to the conventional methods. Therefore, it could be adopted as a cost-effective screening method for the identification of other lentil allergen epitopes ( Kühne et al, 2015 , Li et al, 2021 ).…”

Section: Methodologies For Identification and Quantification Of Lenti...mentioning

confidence: 99%

Lentil allergens identification and quantification: An update from omics perspective

Halima

Najar

Wahab

et al. 2022

Food Chemistry: Molecular Sciences

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“…Protein interactions, such as protein binding and enzyme-substrate reactions, may be studied using functional protein microarrays [26]. Purified recombinant proteins or peptide fragments are assembled into functional arrays that may be utilized for discovery-based research, such as the investigation of protein-protein, protein-DNA, protein-lipid, protein-drug and protein-peptide interactions [27].…”

Section: Functional Protein Microarraymentioning

confidence: 99%

“…Its benefit is that it just takes a little number of proteins to simultaneously investigate numerous analytes inside the original sample. As a result, protein microarrays are extremely effective in the identification of biomarkers [27].…”

Section: Reverse Phase Protein Microarraymentioning

confidence: 99%

Proteomics and Its Role in Diagnosis of Auto Immune Diseases

2022

Al-Azhar University Journal of Virus Researches and Studies

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The "proteome" is the entire protein composition of a cell that is described at a given moment in terms of localisation, interactions, post-translational changes and turnover. Although it is far more complicated than genomics, proteomics is one of the most important methodologies for understanding gene function. It is critical for early illness diagnosis, prognosis and disease progression monitoring. It also plays an important function in medication development as target compounds. Biological fluids obtained from individuals with Auto Immune Diseases (AIDs) may be used to profile autoantibody reactions and proteomics methods have proven to be quite useful in this process. To identify proteins, proteomics methods such as tissue array and mass spectrometry (MS) in various forms are used. Scientists attempting to identify novel biomarkers in illnesses such as AIDs have recently been more interested in protein microarray technology. The goal of this study was to bring attention to the latest developments in proteomic analysis and to determine its function and clinical value in the diagnosis of autoimmune disorders.

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Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases

Cited by 19 publications

References 133 publications

Antibody microarray analysis of the amniotic fluid proteome for predicting the outcome of rescue cerclage in patients with cervical insufficiency

Antibody microarray analysis of the amniotic fluid proteome for predicting the outcome of rescue cerclage in patients with cervical insufficiency

Lentil allergens identification and quantification: An update from omics perspective

Proteomics and Its Role in Diagnosis of Auto Immune Diseases

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