Applications of the polymerase chain reaction (PCR) to the diagnosis of pediatric pulmonary disease

Buck, George E.; Eid, Nemr

doi:10.1002/ppul.1950200510

Cited by 2 publications

(1 citation statement)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method is particularly important for evaluating the sensitivity of chemotherapy (Table 3). Additionally, PCR is also widely used in the diagnosis and treatment of other diseases, such as in parasitic diseases [106], metabolic diseases [107][108][109], autoimmune diseases (rheumatoid arthritis and diabetes) [110], pediatric pulmonary diseases [111], spinal muscular dystrophy diseases [112], and so on. In a word, PCR has been used in almost the whole process of disease prevention, diagnosis, treatment, and prognosis.…”

Section: Applications Of Pcr For Cancer and Other Diseasesmentioning

confidence: 99%

PCR Techniques and Their Clinical Applications

Wang¹,

Cai²,

Chen³

et al. 2024

Biochemistry

View full text Add to dashboard Cite

Kary B. Mullis developed a revolutionary method name polymerase chain reaction (PCR) in 1983, which can synthesize new strand of DNA complementary to the template strand of DNA and produce billions of copies of a DNA fragment only in few hours. Denaturation, annealing, and extension are the three primary steps involved in the PCR process, which generally requires thermocyclers, DNA template, a pair of primers, Taq polymerase, nucleotides, buffers, etc. With the development of PCR, from traditional PCR, quantitative PCR, to next digital PCR, PCR has become a powerful tool in life sciences and medicine. Applications of PCR techniques for infectious diseases include specific or broad-spectrum pathogen detection, assessment and surveillance of emerging infections, early detection of biological threat agents, and antimicrobial resistance analysis. Applications of PCR techniques for genetic diseases include prenatal diagnosis and screening of neonatal genetic diseases. Applications of PCR techniques for cancer research include tumor-related gene detection. This chapter aimed to discuss about the different types of PCR techniques, including traditional PCR, quantitative PCR, digital PCR, etc., and their applications for rapid detection, mutation screen or diagnosis in infectious diseases, inherited diseases, cancer, and other diseases.

show abstract