Applications of three phase partitioning and macro-(affinity ligand) facilitated three phase partitioning in protein refolding

White & grey biotechnologies for shaping a sustainable future

“…These partitioning processes are also useful in protein refolding in cases where the products of the recombinant protein are inactive solids called "inclusion bodies". 50,51 Both TPP and MLFTPP have yet to be tried at the industrial level but show considerable promise as approaches which merge upstream and downstream stages to create streamlined and shorter purication protocols.…”