Applied Orthogonal Experiment Design for Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from<i> Agaricus bisporus</i> Mycelia

Abstract

The purpose of this study to establish the optimum ultrasound-assisted extraction (UAE) parameters of Polysaccharides from Agaricus bisporus mycelia (PABM). Single factor experiment was employed to determine the preliminary range of the extraction parameters including liquid-solid ratio, ultrasonic time, extraction temperature, and ultrasonic power. Then, an orthogonal experiment design was employed to determine the best extraction parameters of PABM. the results showed that the optimal UAE parameters of PABM was liquid-solid ratio of 40 ml/g, ultrasonic time of 70 min, extraction temperature of 55°C, and ultrasonic power of 660 W.