Applying chemical bile duct embolization to achieve chemical hepatectomy in hepatolithiasis: a further experimental study

Ma, Wen‐Jie; Zhou, Yong; Shrestha, Anuj; Mao, Hui; Li, Fuyu; Cheng, Nan‐Sheng; Zhang, Wei; Xu, Rui‐Hua; Zhang, Yong-Qiong; Jiang, Ting; Feng, Huan; Li, Wen; Han, Qiang

doi:10.1016/j.jss.2013.10.053

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…The hyperplastic peribiliary glands and blood vessels around these glands are usually enclosed or obstructed by the condensed fibrous connect tissue to form intricate branches. Thus, bacteria could hide in these branches and cause new biliary infection which leads to repeated and persistent CPC [ 1 , 16 , 28 ]. More importantly, the proliferation of fibrous connective tissue in the bile duct wall could narrow the biliary lumen to form bile duct strictures, which would slow the bile flow and lead to bile stasis.…”

Section: Discussionmentioning

confidence: 99%

Effective Treatment of Chronic Proliferative Cholangitis by Local Gentamicin Infusion in Rabbits

Yang

et al. 2018

BioMed Research International

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Background Hepatolithiasis is highly prevalent in East Asia characterized by the presence of gallstones in the biliary ducts of the liver. Surgical resection is the potentially curative treatment but bears a high risk of stone recurrence and biliary restenosis. This is closely related to the universal presence of chronic proliferative cholangitis (CPC) in the majority of patients. Recent evidence has indicated the association of bacterial infection with the development of CPC in hepatolithiasis. Thus, this study aims to investigate the feasibility and efficacy of local infusion of gentamicin (an antibiotic) for the treatment of CPC in a rabbit model. Methods The rabbit CPC model was established based on previously published protocols. Bile duct samples were collected from gentamicin-treated or control animals for pathological and molecular characterization. Results Histologically, the hyperplasia of biliary epithelium and submucosal glands were inhibited and the thickness of the bile duct wall was significantly decreased after gentamicin therapy. Consistently, the percentage of proliferating cells marked by ki67 was significantly reduced by the treatment. More importantly, this treatment inhibited interleukin 2 production, an essential inflammatory cytokine, and the enzyme activity of endogenous β-Glucuronidase, a key factor in the formation of bile pigment. Conclusions Local gentamicin infusion effectively inhibits the inflammation, cell proliferation, and lithogenesis in a rabbit model of CPC. This approach represents a potential treatment for CPC and thus prevents recurrent hepatolithiasis.

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Section: Discussionmentioning

confidence: 99%

Effective Treatment of Chronic Proliferative Cholangitis by Local Gentamicin Infusion in Rabbits

Yang

et al. 2018

BioMed Research International

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“…Histologically, the glands in the bile duct wall and around the bile duct in the liver of hepatolithiasis showed obvious proliferative pathological changes with in ammation and brosis (8). Hyperplastic glands and bile duct epithelium produce a large amount of mucin, which provides a suitable microenvironment for the formation of stones (15). Starting the process of stone formation and growth with calcium salt and lipid as the core is an important mechanism of chronic hyperplastic cholangitis and stone formation (16).…”

Section: Discussionmentioning

confidence: 99%

IL-19 induces MUC5AC overproduction in hepatolithiasis via stat3 pathway

WEI

2022

Preprint

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Background Intrahepatic biliary lithiasis is a benign biliary disease with complex pathological changes, high recurrence rate and difficult to cure. The pathological mechanism underlying hepatolithiasis development is closely related to chronic inflammation of intrahepatic bile duct and aberrant expression of mucin5ac (MUC5AC). The present study aimed to verify the up-regulation effect of IL-19 to MUC5AC in hepatolithiasis. Results He staining, PAS staining, immunohistochemistry and immunofluorescence were used to detect the expression of MUC5AC and IL-19 in bile duct tissue. The expressions of MUC5AC and STAT3 in bile duct tissue, epithelial cells and cell supernatant were detected by Western blot, PCR and ELISA. Chip experiment verified the binding of STAT3 to MUC5AC promoter sequence. The regulation effect of the stat3 signaling pathway was examined by stat3 siRNA transfection and using cryptotanshinone (CRY, a stat3 inhibitor ). MUC5AC secretion level were significantly increased in mucosa of hepatolithiasis tissue compared to controls. After pretreatment with rhIL-19, the expression level of MUC5AC in HEiECs was significantly up-regulated. Knockdown of IL-20R or pretreatment with CRY attenuated the up-regulation effect of IL-19 to MUC5AC. Conclusions The present study suggest that the IL-19 can induce MUC5AC overproduction in hepatolithiasis via stat3 pathway. Intervention of IL-19 and its downstream molecules may provide support for the treatment and prevention of hepatolithiasis.

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“…Cell lysis and western blotting were carried out as previously described [24 , 28] . Antibodies recognizing the following proteins were used: Akt (1:1000, Boster, Wuhan, China), p-Akt (Thr308) (1:1000, Cell Signaling Technology), FOXO1 (1:1000, Abcam), p-FOXO1 (S256) (1:1000, Abcam), AMPKα (1:1000, Cell Signaling Technology), c-Myc (1:1000, Boster), C-C motif chemokine ligand 2 (CCL2) (1:1000, Abcam), C-C motif chemokine ligand 10 (CXCL10) (1:1000, Abcam), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:2000, Abways Technology, China).…”

Section: Methodsmentioning

confidence: 99%

PI3K-CCL2-CCR2-MDSCs axis: A potential pathway for tumor Clostridia-promoted CD 8+ T lymphocyte infiltration in bile tract cancers

Ma,

Li,

et al. 2023

Neoplasia

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Applying chemical bile duct embolization to achieve chemical hepatectomy in hepatolithiasis: a further experimental study

Cited by 10 publications

References 22 publications

Effective Treatment of Chronic Proliferative Cholangitis by Local Gentamicin Infusion in Rabbits

Effective Treatment of Chronic Proliferative Cholangitis by Local Gentamicin Infusion in Rabbits

IL-19 induces MUC5AC overproduction in hepatolithiasis via stat3 pathway

PI3K-CCL2-CCR2-MDSCs axis: A potential pathway for tumor Clostridia-promoted CD 8+ T lymphocyte infiltration in bile tract cancers

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