Applying high-resolution melting (HRM) technology to olive oil and wine authenticity

Pereira, L. S.; Gomes, Sónia; Barrias, Sara; Fernandes, José R.; Martins-Lopes, Paula

doi:10.1016/j.foodres.2017.10.026

Cited by 50 publications

(27 citation statements)

References 90 publications

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“…In Europe, traceability systems are applied to promote and protect certain denominations, such as the Protected Designation of Origin (PDO), Protected Geographical Indication (PGI), and Traditional Specialties Guaranteed (TSG). These designations of origin are conferred to high quality agricultural products, like wine, which are strictly linked to their origin area and specific viticulture and oenological practices [7,8].…”

Section: Wine Authenticitymentioning

confidence: 99%

“…Nevertheless, due to recent technological advancements, it is possible to detect and discriminate the grapevine variety present in such sample types [4,8,75]. In an authenticity system, it is crucial to establish a framework concerning the most suitable sampling and storage conditions regarding the subsequent steps and the final defined purpose (Figure 3).…”

Section: Dna Fingerprinting For Varietal Identificationmentioning

confidence: 99%

“…Nowadays, technologies based on genomics and bioinformatics approaches are considered the most efficient tools for assessing the genetic authenticity of food products, and, therefore, their incorporation in traceability systems is highly advantageous (Figure 3). Among the DNA-based technologies, High Resolution Melting (HRM) has been shown to be an interesting technology for food authenticity purposes [8]. The recent advances on the instrumentation utilized, as well as on specialized and more efficient fluorescent DNA-binding dyes, have allowed this technique to become a high-throughput screening assay for grapevine varietal identification and wine analysis [8,80,83].…”

Section: Dna Fingerprinting For Varietal Identificationmentioning

confidence: 99%

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From the Field to the Bottle—An Integrated Strategy for Wine Authenticity

et al. 2018

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The wine sector is one of the most economically important agro-food businesses. The wine market value is largely associated to terroir, in some cases resulting in highly expensive wines that attract fraudulent practices. The existent wine traceability system has some limitations that can be overcome with the development of new technological approaches that can tackle this problem with several means. This review aims to call attention to the problem and to present several strategies that can assure a more reliable and authentic wine system, identifying existent technologies developed for the sector, which can be incorporated into the current traceability system.

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Section: Wine Authenticitymentioning

confidence: 99%

Section: Dna Fingerprinting For Varietal Identificationmentioning

confidence: 99%

Section: Dna Fingerprinting For Varietal Identificationmentioning

confidence: 99%

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From the Field to the Bottle—An Integrated Strategy for Wine Authenticity

et al. 2018

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“…25,26 High-resolution melting (HRM) analysis is a cost-effective and reliable method for the detection of differences in the DNA sequence. This method has been used with oil, 27,28 wine, 29 herbal medicines, 30,31 and other animal and plant species in foods, 32,33 without requiring further sequencing. The combination of DNA barcoding and HRM analysis is used increasingly in food tracing due to its low cost, its high throughput, and the fact that results are obtainable in less than 3 h. 28,34 In this study, we used the psbA-trnH barcode combined with HRM analysis to identify common adulterants (peanut and soybean) in walnut milk beverage.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

et al. 2020

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Background Walnut (Juglans regia L.) is one of the most widely cultivated nuts. Walnut milk beverage is very popular in China due to its nutritional value. However, adulterated walnut milk ingredients have been detected in the Chinese market. Peanut and soybean are sold at much lower prices than walnut and are reported to be commonly used for adulteration in the industrial chain of walnut milk production. The purpose of this study is therefore to develop an accurate and efficient method for detecting the authenticity of the raw materials used in walnut milk beverage. Results DNA barcoding and high‐resolution melting (HRM) analyses were used to identify common adulterated raw ingredients such as peanut and soybean in commercial walnut milk beverage samples. The chloroplast psbA‐trnH gene was used for sequencing, and HRM analysis was performed. We also prepared experimental mixtures, in the laboratory, with different quantities of walnut, peanut, and soybean. High‐resolution melting analysis of the experimental mixtures clearly distinguished all of them. The results revealed that most of the walnut milk beverage samples fell in the same cluster of walnut species. Several samples fell in the peanut cluster, confirming that they were adulterated products. Conclusion The results revealed that HRM analysis based on the psbA‐trnH barcode sequence can be used to identify raw ingredients in walnut milk beverages. © 2020 Society of Chemical Industry

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“…The technique is so sensitive that it can detect single base differences (single nucleotide polymorphisms, SNPs) in sequences and is used for mutation screening and SNP genotyping (e.g., Garritano et al, 2009). HRM analysis has been widely used in the food research industry for identification of plant or animal materials due to its high throughput potential (Odell et al, 2005;Jeong et al, 2010;Malewski et al, 2010;Ramón-Laca et al, 2014;Simko, 2016;Pereira et al, 2017Pereira et al, , 2018. The use of multiple amplicons or barcodes (multi Bar-HRM) in a multiplexed reaction is also possible, and successfully discriminated ∼30 distinct plant families (Ballin et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Rapid Genetic Identification of the Blue Crab Callinectes sapidus and Other Callinectes spp. Using Restriction Enzyme Digestion and High Resolution Melt (HRM) Assays

et al. 2020

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The blue crab Callinectes sapidus is one of the most widely studied marine crustaceans due to its high economic value and ecological significance. Despite extensive research on the blue crab in North America, many questions remain about the distribution and abundance of the species in the subtropics and tropics. In many places, C. sapidus is sympatric with morphologically similar Callinectes spp., which has implications for seafood mislabeling. To enable rapid identification of the species, we designed and tested two PCR-based assays targeting the 12S rRNA mitochondrial gene. The first assay discriminates C. sapidus from other Callinectes spp. via post-PCR restriction digestion (PCR-RFLP) and the second assay discriminates among multiple Callinectes spp. through High Resolution Melting (HRM) analysis and supervised machine learning analyses. A total of 58 DNA samples from five Callinectes spp. (validated via 12S gene sequencing) were used for assay testing. The PCR RFLP assay was 100% accurate identifying C. sapidus from other Callinectes spp. HRM analysis of amplicons showed good discrimination among species, with distinct clusters formed between species with higher sequence homology. Linear discriminant analysis (LDA) classification of HRM curves was quite successful given the small dataset available, producing ∼90-91% mean accuracy in classification over all species with 100-fold cross validation. Much of the error came from misclassifications between C. similis and C. danae, which are ∼99% similar in sequence for the amplicon; collapsing them into a single class increased overall classification success to 94%. Error also arose from C. bocourti classifications, which had a reference set containing only three samples. Classification accuracy of C. sapidus alone via HRM was 97.5%. Overall, these assays show great promise as rapid and inexpensive methods to identify Callinectes spp. and have application for both ecological research and seafood identification or labeling.

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Applying high-resolution melting (HRM) technology to olive oil and wine authenticity

Cited by 50 publications

References 90 publications

From the Field to the Bottle—An Integrated Strategy for Wine Authenticity

From the Field to the Bottle—An Integrated Strategy for Wine Authenticity

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

Rapid Genetic Identification of the Blue Crab Callinectes sapidus and Other Callinectes spp. Using Restriction Enzyme Digestion and High Resolution Melt (HRM) Assays

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