Applying synthetic biology strategies to bioelectrochemical systems

Dong, Fangyuan; Simoska, Olja; Gaffney, Erin M.

doi:10.1002/elsa.202100197

Cited by 11 publications

(10 citation statements)

References 86 publications

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“…Specifically, the engineered bacteria with OmcS allowed a 13-fold higher NH 3 production compared to the strain not engineered with the heterologous EET pathway, together with an increased faradaic efficiency of about 23%. We refer the reader to recent reviews on the use of synthetic biology for microbial electrochemical systems for further insights into this topic. , …”

Section: Principles Of Bioelectrochemistrymentioning

confidence: 99%

Enzymatic and Microbial Electrochemistry: Approaches and Methods

et al. 2022

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The coupling of enzymes and/or intact bacteria with electrodes has been vastly investigated due to the wide range of existing applications. These span from biomedical and biosensing to energy production purposes and bioelectrosynthesis, whether for theoretical research or pure applied industrial processes. Both enzymes and bacteria offer a potential biotechnological alternative to noble/rare metal-dependent catalytic processes. However, when developing these biohybrid electrochemical systems, it is of the utmost importance to investigate how the approaches utilized to couple biocatalysts and electrodes influence the resulting bioelectrocatalytic response. Accordingly, this tutorial review starts by recalling some basic principles and applications of bioelectrochemistry, presenting the electrode and/or biocatalyst modifications that facilitate the interaction between the biotic and abiotic components of bioelectrochemical systems. Focus is then directed toward the methods used to evaluate the effectiveness of enzyme/bacteria−electrode interaction and the insights that they provide. The basic concepts of electrochemical methods widely employed in enzymatic and microbial electrochemistry, such as amperometry and voltammetry, are initially presented to later focus on various complementary methods such as spectroelectrochemistry, fluorescence spectroscopy and microscopy, and surface analytical/characterization techniques such as quartz crystal microbalance and atomic force microscopy. The tutorial review is thus aimed at students and graduate students approaching the field of enzymatic and microbial electrochemistry, while also providing a critical and up-to-date reference for senior researchers working in the field.

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Section: Principles Of Bioelectrochemistrymentioning

confidence: 99%

Enzymatic and Microbial Electrochemistry: Approaches and Methods

et al. 2022

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“…Moreover, electroactive engineered bacteria can be prepared using synthetic biology, either to improve ETT or to add new non‐native enzyme pathways. A recent review described all of these genetic engineering strategies [26]. During ETT process, electrode serves either as an electron acceptor for exoelectrogenic bacteria (Bioanode) or as an electron donor for electrotophic bacteria (Biocathode) (Figures 2).…”

Section: Sensing With Eabmentioning

confidence: 99%

Recent Advances on Electrochemical Sensors Based on Electroactive Bacterial Systems for Toxicant Monitoring: A Minireview

Haddour

Azri

2022

Electroanalysis

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In recent years, there have been advancements in the development of bacterial electrochemical sensors for toxicity monitoring, especially through utilization of electroactive bacteria. Accordingly, this mini review summarizes the recent advances in the design of bacterialbased electrochemical sensors with a specific discussion of main methodologies used for preparation of bioelectrodes based on electroactive bacteria. Additionally, current trends in the design of efficient and high performing bacterial electrochemical sensors for toxicity monitoring are presented. An overview of the most relevant findings and challenges of this technology for practical are provided and might serve as a general outlook for planning further research.

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“…coli cells have been successfully modified to transfer electrons to anodes via the expression of Shewanella oneidensis methionine synthase biosynthesis. , Using E . coli has various advantages, including rapid cellular growth rates and relatively simple cell culturing methods, as well as the capability to metabolically oxidize a wide range of substrates, such as glucose, malic acid, lactose, fumarate, and sucrose, among many others. ,, Recognized for their high electron transfer abilities, phenazines are nitrogen-containing heterocyclic compounds produced as secondary metabolites by Pseudomonas species. − Using biosynthetic phenazine-modifying genes, Pseudomonas aeruginosa produces at least five distinct phenazines, including phenazine-1-carboxylic acid (PCA), pyocyanin (PYO), 5-methylphenazine-1-carboxylic acid (5-MCA), and 1-hydroxyphenazine (OHPHZ) in different micromolar amounts. − This phenazine metabolite production depends on external environmental parameters (e.g., O 2 availability, carbon sources, pH). , In the P . aeruginosa phenazine biosynthetic pathway, PCA is produced via a seven-gene phenazine biosynthetic cluster, designated as phzA-G .…”

Section: Introductionmentioning

confidence: 99%

“…42,43 Using E. coli has various advantages, including rapid cellular growth rates and relatively simple cell culturing methods, as well as the capability to metabolically oxidize a wide range of substrates, such as glucose, malic acid, lactose, fumarate, and sucrose, among many others. 19,41,44 Recognized for their high electron transfer abilities, phenazines are nitrogen-containing heterocyclic compounds produced as secondary metabolites by Pseudomonas species. 45−47 Using biosynthetic phenazine-modifying genes, Pseudomonas aeruginosa produces at least five distinct phenazines, including phenazine-1-carboxylic acid (PCA), pyocyanin (PYO), 5methylphenazine-1-carboxylic acid (5-MCA), and 1-hydroxyphenazine (OHPHZ) in different micromolar amounts.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Finally, the regular addition of exogenous mediators to an MFC system is not only environmentally challenging, but also technologically unfeasible. Another strategy is to employ endogenous electron mediators, such as flavins, phenazines, and quinones, all of which can be synthesized by various microbes. − Microorganisms capable of self-generating their redox mediators can directly transfer electrons to anode surfaces, enabling simplified MFC design and device operation at high-sustained activity levels.…”

Section: Introductionmentioning

confidence: 99%

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Enhancing the Performance of Microbial Fuel Cells via Metabolic Engineering of Escherichia coli for Phenazine Production

Simoska,

Cummings,

Gaffney

et al. 2023

ACS Sustainable Chem. Eng.

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One of the central roles in the design, development, and application advances in mediator-based microbial electrochemical systems, such as microbial fuel cells (MFCs), is the establishment of efficient and successful communication between conductive electrode surfaces and microorganisms via modes of extracellular electron transfer (EET). Most microbial-based systems require the use of artificial electroactive mediators in order to facilitate and/or enhance electron transfer. Our previous work established an exogenous phenazine-based library as a mediator system to enable EET from the model microorganism Escherichia coli as a promising biotechnological host. However, the addition of exogenous mediators to a microbial electrochemical system has certain limiting downsides, specifically with regard to mediator toxicity to cells and increased operational expenses. Herein, we demonstrate the metabolic and genetic engineering of E. coli to self-generate phenazine metabolites endogenously by introducing the phenazine biosynthetic pathway from P. aeruginosa into E. coli. This biosynthetic pathway contains a phenazine cluster of seven genes, namely, phzABCDEFG (phzA-G), responsible for the synthetic conversion of phenazine-1-carboxylic acid (PCA) from chorismic acid, and two additional phenazine accessory genes phzM and phzS to catalyze the transformation of PCA to pyocyanin (PYO). We present the characterization of the engineered E. coli cells that were collected via electrochemical measurements, RNA sequencing, and microscopy imaging. Finally, the engineered E. coli cells were used for the design of a microbial fuel cell with enhanced performances, demonstrating a maximum power density increase from 127 ± 5 mW m −2 with nonengineered E. coli cells to 806 ± 7 mW m −2 with genetically engineered, phenazine-producing E. coli. Our results indicate that the introduction of a heterologous electron shuttle into E. coli is not only an efficient, but also a promising strategy toward establishing efficacious electron mediation in living bioelectrochemical systems and enhancing the overall MFC performance related to the MFC current generation and power output.

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Applying synthetic biology strategies to bioelectrochemical systems

Cited by 11 publications

References 86 publications

Enzymatic and Microbial Electrochemistry: Approaches and Methods

Enzymatic and Microbial Electrochemistry: Approaches and Methods

Recent Advances on Electrochemical Sensors Based on Electroactive Bacterial Systems for Toxicant Monitoring: A Minireview

Enhancing the Performance of Microbial Fuel Cells via Metabolic Engineering of Escherichia coli for Phenazine Production

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