Appraisal of biochemical and genetic diversity of mango cultivars using molecular markers

Abou-Ellail, Mohamed; El-Shabrawi, Hattem M.; Matter, Mohamed A.; Aly, Usama I; Ghareeb, Hassan; Eissa, E. A.

doi:10.5897/ajb2014.13685

Cited by 4 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, protein markers are useful tools to identify cultivar and classification of crop species to study genetic diversity, thereby improving the efficiency of plant breeding programs (Gianibelli et al, 2001;Kuťka-Hlozákovás et al, 2016;Pe´rez de, 1993;Sharma and Krishna, 2017). Among biochemical techniques, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) due to its simplicity, minimum cost in time and labor, and effectiveness is commonly used in the studies of plants genetic diversity (Abou-Ellail et al, 2014;Ahmed et al, 2010;Masoumi et al, 2012;Radwan et al, 2013;Sadia et al, 2009). Since this technique is non-destructive and needs only a small amount of tissue to obtain sufficient material for analysis and the rest of the seed could be grown into mature plants and seed can be obtained from the tested seeds.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Genetic Diversity in Barley (Hordeum vulgare) Genotypes using Protein Profiling

Eid

2018

J. Plant Breed. Genet.

View full text Add to dashboard Cite

The knowledge of the genetic diversity of barley (Hordeum vulgare) genotypes based on protein polymorphism is very important for breeding programs. The purpose of the current study was to determine the genetic diversity and relationships among ten barley genotypes by using Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for protein profiles. A total number of 30 bands with molecular weights ranging from 12 to 148 KD were detected. Out of these, five bands were observed monomorphic. Rest of the bands had shown polymorphism to the extent of 83.3% among the test genotypes. The genetic similarity of the ten genotypes tested varied from 0.26 to 1.00 with an average of 0.51. Cluster analysis divided the ten genotypes into two major clusters comprising four subclusters, which was consistent with the systematic classification of barley done in previous studies. The results of this study indicated that the genotypes of barley could effectively be differentiated based on polymorphism, detected between protein patterns. SDS-PAGE presented a higher differentiation power and better repeatability; thus, could be used as a rapid and reliable method for genetic diversity analysis and laid a solid foundation for future barley breeding.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Genetic Diversity in Barley (Hordeum vulgare) Genotypes using Protein Profiling

Eid

2018

J. Plant Breed. Genet.

View full text Add to dashboard Cite

show abstract

“…The number of amplified fragments varied from 3 (OPG 13) to 20 (OPX 04) with an average of 11.8 polymorphic fragments per primer (Table 1). Schnell and Knight (1993), Kumar et al (2001), Xie Jiang Hui et al (2005), Anuj et al (2007), Bajpai et al (2008), Fitmawati and Puwoko, (2010), Gajera et al (2011), Roy and Abhishek (2011) and Abou-Ellial et al (2014) reported amplification of 10. 90, 13.90, 11.70, 10.53, 12.22, 9.28, 8.90, 10.66 and 16.10 bands per primer, respectively in mango.…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of traditional mango germplasm of coastal Andhra Pradesh using RAPD markers

2021

EJPB

View full text Add to dashboard Cite

A study was conducted at Horticultural research station, Venkataramannagudem for evaluating the variability of mango germplasm to conserve the elite ones and to identify the superior genotypes using molecular markers for future crop improvement. Genetic variation and relationship among 34 traditional mango germplasm were analyzed using Random Amplified Polymorphic DNA (RAPD) markers. Fifteen out of the twenty primers screened were informative and 177 amplified DNA bands with sizes ranging from 100 -5000 bp were selected as RAPD markers. The number of amplified fragments varied from 3 (OPG 13) to 20 (OPX 04) with an average of 11.8 polymorphic fragments per marker. Specific RAPD markers for some mango germplasm were identified. Unweighted Pair Group Method using Arithmetic means (UPGMA) cluster analysis grouped all the germplasm into two clusters with a genetic similarity coefficient range of 0.67 to 0.88. The accessions within the germplasm i.e., Banganapalli -1, Banganapalli -2 and Banginapalli-3 showed genetic similarity which is in good agreement from a single ancestor with their putative pedigrees. The genotypes Panukula Mamidi and Rajamamidi were closely clustered since these two genotypes are native to the Vizianagaram district. Similarly, the genotypes Nuzividu Tiyya Mamidi and Nuzividu Rasalu occurred in the same cluster indicated that these genotypes also originated from the same geographical area, it can be concluded that they were separated into the cluster based on geographic origin. This study showed clearly that germplasm from coastal Andhra Pradesh unveiled maximum diversity and indicated the potential of RAPD markers for the identification of mango germplasm for breeding purposes.

show abstract

“…Information about the chlorophyll contents of the plant accessions can be used as an indicator of plant vigor and contribute significantly in improving the plant biomass and grain yield [38]. It has been reported that the amount of these pigments are associated with genetical and environmental factor [56] and related to the photosystem II and oxidative stress of the plant [57,58]. In the present study, the accessions exhibited a wide range of variation in relative chlorophyll pigment (Chl a, Chl b, and ratio of Chl a/chl b).…”

Section: Discussionmentioning

confidence: 99%

Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits

Ghimire

Kim

et al. 2019

Molecules

View full text Add to dashboard Cite

The genetic diversity and antioxidant potential of Panicum miliaceum L. accessions collected from different geo-ecological regions of South Korea were evaluated and compared. Antioxidant potential of seeds was estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging assays and total phenolic content was determined by the Folin–Ciocalteu method. Total phenolic content (TPC) in 80% methanolic extracts ranged from 16.24 ± 0.86 to 58.04 ± 1.00 mg gallic acid equivalent (GAE)/g of the sample extracts and total flavonoid content (TFC) varied from 7.19 ± 1.05 to 52.56 ± 1.50 mg quercetin equivalents (QE) mg/g of the sample extracts. DPPH radical scavenging capacity of the extracts from the 15 accessions of P. miliaceum varied from 206.44 ± 7.72 to 2490.24 ± 4.641 mg GAE/g of the sample extracts and ABTS radical scavenging capacity ranged from 624.85 ± 13.1 to 1087. 77 ± 9.58 mg GAE/g of the sample extracts. A wide range of genetic variation was observed as measured by Shannon’s information index (I), number of effective alleles (Ne), number of observed alleles (Na), expected heterozygosity (He), unbiased expected heterozygosity (uHe). The observed variation in the bioactive properties, morphological traits, and genetic diversity among the accessions may provide useful information for breeding programs seeking to improve bioactive properties of P. miliaceum.

show abstract

Appraisal of biochemical and genetic diversity of mango cultivars using molecular markers

Cited by 4 publications

References 39 publications

Evaluation of Genetic Diversity in Barley (Hordeum vulgare) Genotypes using Protein Profiling

Evaluation of Genetic Diversity in Barley (Hordeum vulgare) Genotypes using Protein Profiling

Molecular characterization of traditional mango germplasm of coastal Andhra Pradesh using RAPD markers

Diversity in Accessions of Panicum miliaceum L. Based on Agro-Morphological, Antioxidative, and Genetic Traits

Contact Info

Product

Resources

About